Scanless functional imaging of hippocampal networks using patterned two-photon illumination through GRIN lenses

Handle URI:
http://hdl.handle.net/10754/622598
Title:
Scanless functional imaging of hippocampal networks using patterned two-photon illumination through GRIN lenses
Authors:
Moretti, Claudio; Antonini, Andrea; Bovetti, Serena; Liberale, Carlo ( 0000-0002-5653-199X ) ; Fellin, Tommaso
Abstract:
Patterned illumination through the phase modulation of light is increasingly recognized as a powerful tool to investigate biological tissues in combination with two-photon excitation and light-sensitive molecules. However, to date two-photon patterned illumination has only been coupled to traditional microscope objectives, thus limiting the applicability of these methods to superficial biological structures. Here, we show that phase modulation can be used to efficiently project complex two-photon light patterns, including arrays of points and large shapes, in the focal plane of graded index (GRIN) lenses. Moreover, using this approach in combination with the genetically encoded calcium indicator GCaMP6, we validate our system performing scanless functional imaging in rodent hippocampal networks in vivo ~1.2 mm below the brain surface. Our results open the way to the application of patterned illumination approaches to deep regions of highly scattering biological tissues, such as the mammalian brain.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Moretti C, Antonini A, Bovetti S, Liberale C, Fellin T (2016) Scanless functional imaging of hippocampal networks using patterned two-photon illumination through GRIN lenses. Biomedical Optics Express 7: 3958. Available: http://dx.doi.org/10.1364/BOE.7.003958.
Publisher:
The Optical Society
Journal:
Biomedical Optics Express
Issue Date:
12-Sep-2016
DOI:
10.1364/BOE.7.003958
Type:
Article
ISSN:
2156-7085; 2156-7085
Sponsors:
Istituto Italiano di Tecnologia "Interdisciplinary - Interdepartmental project" to CL and TF and grants from European Research Council (ERC, NEURO-PATTERNS), FP7-Health (DESIRE), MIUR FIRB (RBAP11X42L), and NIH Brain Initiative (1U01NS090576-01) to TF.
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMoretti, Claudioen
dc.contributor.authorAntonini, Andreaen
dc.contributor.authorBovetti, Serenaen
dc.contributor.authorLiberale, Carloen
dc.contributor.authorFellin, Tommasoen
dc.date.accessioned2017-01-02T09:55:32Z-
dc.date.available2017-01-02T09:55:32Z-
dc.date.issued2016-09-12en
dc.identifier.citationMoretti C, Antonini A, Bovetti S, Liberale C, Fellin T (2016) Scanless functional imaging of hippocampal networks using patterned two-photon illumination through GRIN lenses. Biomedical Optics Express 7: 3958. Available: http://dx.doi.org/10.1364/BOE.7.003958.en
dc.identifier.issn2156-7085en
dc.identifier.issn2156-7085en
dc.identifier.doi10.1364/BOE.7.003958en
dc.identifier.urihttp://hdl.handle.net/10754/622598-
dc.description.abstractPatterned illumination through the phase modulation of light is increasingly recognized as a powerful tool to investigate biological tissues in combination with two-photon excitation and light-sensitive molecules. However, to date two-photon patterned illumination has only been coupled to traditional microscope objectives, thus limiting the applicability of these methods to superficial biological structures. Here, we show that phase modulation can be used to efficiently project complex two-photon light patterns, including arrays of points and large shapes, in the focal plane of graded index (GRIN) lenses. Moreover, using this approach in combination with the genetically encoded calcium indicator GCaMP6, we validate our system performing scanless functional imaging in rodent hippocampal networks in vivo ~1.2 mm below the brain surface. Our results open the way to the application of patterned illumination approaches to deep regions of highly scattering biological tissues, such as the mammalian brain.en
dc.description.sponsorshipIstituto Italiano di Tecnologia "Interdisciplinary - Interdepartmental project" to CL and TF and grants from European Research Council (ERC, NEURO-PATTERNS), FP7-Health (DESIRE), MIUR FIRB (RBAP11X42L), and NIH Brain Initiative (1U01NS090576-01) to TF.en
dc.publisherThe Optical Societyen
dc.subjectEndoscopic imagingen
dc.subjectFluorescence microscopyen
dc.subjectNonlinear microscopyen
dc.subjectPhase modulationen
dc.titleScanless functional imaging of hippocampal networks using patterned two-photon illumination through GRIN lensesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalBiomedical Optics Expressen
dc.contributor.institutionOptical Approaches to Brain Function Laboratory, Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30, Genova, 16163, Italyen
dc.contributor.institutionNanostructures Department, Istituto Italiano di Tecnologia, Via Morego 30, Genova, 16163, Italyen
kaust.authorLiberale, Carloen
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