Development of a sensing system to detect C-telopeptide of type-I collagen

Handle URI:
http://hdl.handle.net/10754/622533
Title:
Development of a sensing system to detect C-telopeptide of type-I collagen
Authors:
Afsarimanesh, Nasrin; Zia, Asif I.; Mukhopadhyay, S. C.; Kruger, Marlena; Yu, P. L.; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.
KAUST Department:
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Electrical Engineering Program
Citation:
Afsarimanesh N, Zia AI, Mukhopadhyay SC, Kruger M, Yu PL, et al. (2015) Development of a sensing system to detect C-telopeptide of type-I collagen. 2015 9th International Conference on Sensing Technology (ICST). Available: http://dx.doi.org/10.1109/ICSensT.2015.7438466.
Publisher:
Institute of Electrical and Electronics Engineers (IEEE)
Journal:
2015 9th International Conference on Sensing Technology (ICST)
Conference/Event name:
9th International Conference on Sensing Technology, ICST 2015
Issue Date:
30-Mar-2016
DOI:
10.1109/ICSensT.2015.7438466
Type:
Conference Paper
Appears in Collections:
Conference Papers; Electrical Engineering Program; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorAfsarimanesh, Nasrinen
dc.contributor.authorZia, Asif I.en
dc.contributor.authorMukhopadhyay, S. C.en
dc.contributor.authorKruger, Marlenaen
dc.contributor.authorYu, P. L.en
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2017-01-02T09:55:28Z-
dc.date.available2017-01-02T09:55:28Z-
dc.date.issued2016-03-30en
dc.identifier.citationAfsarimanesh N, Zia AI, Mukhopadhyay SC, Kruger M, Yu PL, et al. (2015) Development of a sensing system to detect C-telopeptide of type-I collagen. 2015 9th International Conference on Sensing Technology (ICST). Available: http://dx.doi.org/10.1109/ICSensT.2015.7438466.en
dc.identifier.doi10.1109/ICSensT.2015.7438466en
dc.identifier.urihttp://hdl.handle.net/10754/622533-
dc.description.abstractThis research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.en
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en
dc.subjectElectrochemical Impedance Spectroscopyen
dc.subjectELISAen
dc.subjectinterdigital sensoren
dc.subjectosteoporosisen
dc.titleDevelopment of a sensing system to detect C-telopeptide of type-I collagenen
dc.typeConference Paperen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentElectrical Engineering Programen
dc.identifier.journal2015 9th International Conference on Sensing Technology (ICST)en
dc.conference.date2015-12-08 to 2015-12-11en
dc.conference.name9th International Conference on Sensing Technology, ICST 2015en
dc.conference.locationAuckland, NZLen
dc.contributor.institutionSchool of Engineering and Advanced Technology, Massey University, Palmerston North, New Zealanden
dc.contributor.institutionInstitute of Food Science and Technology, Massey University, Palmerston North, New Zealanden
kaust.authorKosel, Jürgenen
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