A study of the incubation of microbead agglutination assays in a microfluidic system

Handle URI:
http://hdl.handle.net/10754/622509
Title:
A study of the incubation of microbead agglutination assays in a microfluidic system
Authors:
Castro, David; Conchouso Gonzalez, David ( 0000-0002-9788-0977 ) ; Carreno, Armando Arpys Arevalo ( 0000-0001-9446-3310 ) ; Foulds, Ian G.
Abstract:
This work reports on a quantitative study of the incubation of a microbead-based agglutination assay inside a microfluidic system. In this system, a droplet (1.25µL) consisting of a mixture of functionalized microbeads and analyte is flowed through a 0.51mm internal diameter silicone tube. Hydrodynamic forces alone produce a very efficient mixing of the beads within the droplet. We tested the agglutination at different speeds and show a robust response at the higher range of speeds (150 – 200µL/min), while also reaching a completion in the agglutination process. At these velocities, a length of 180cm is shown to be sufficient to confidently measure the agglutination assay, which takes between 2.5 – 3 minutes. This high throughput quantification method has the potential of accelerating the measurements of various types of biomarkers, which can greatly benefit the fields of biology and medicine.
KAUST Department:
King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia
Citation:
Castro D, Conchouso D, Arevalo A, Foulds IG (2016) A study of the incubation of microbead agglutination assays in a microfluidic system. 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). Available: http://dx.doi.org/10.1109/NEMS.2016.7758266.
Publisher:
Institute of Electrical and Electronics Engineers (IEEE)
Journal:
2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)
Issue Date:
19-Dec-2016
DOI:
10.1109/NEMS.2016.7758266
Type:
Conference Paper
Additional Links:
http://ieeexplore.ieee.org/document/7758266/
Appears in Collections:
Conference Papers

Full metadata record

DC FieldValue Language
dc.contributor.authorCastro, Daviden
dc.contributor.authorConchouso Gonzalez, Daviden
dc.contributor.authorCarreno, Armando Arpys Arevaloen
dc.contributor.authorFoulds, Ian G.en
dc.date.accessioned2017-01-02T09:55:27Z-
dc.date.available2017-01-02T09:55:27Z-
dc.date.issued2016-12-19en
dc.identifier.citationCastro D, Conchouso D, Arevalo A, Foulds IG (2016) A study of the incubation of microbead agglutination assays in a microfluidic system. 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). Available: http://dx.doi.org/10.1109/NEMS.2016.7758266.en
dc.identifier.doi10.1109/NEMS.2016.7758266en
dc.identifier.urihttp://hdl.handle.net/10754/622509-
dc.description.abstractThis work reports on a quantitative study of the incubation of a microbead-based agglutination assay inside a microfluidic system. In this system, a droplet (1.25µL) consisting of a mixture of functionalized microbeads and analyte is flowed through a 0.51mm internal diameter silicone tube. Hydrodynamic forces alone produce a very efficient mixing of the beads within the droplet. We tested the agglutination at different speeds and show a robust response at the higher range of speeds (150 – 200µL/min), while also reaching a completion in the agglutination process. At these velocities, a length of 180cm is shown to be sufficient to confidently measure the agglutination assay, which takes between 2.5 – 3 minutes. This high throughput quantification method has the potential of accelerating the measurements of various types of biomarkers, which can greatly benefit the fields of biology and medicine.en
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en
dc.relation.urlhttp://ieeexplore.ieee.org/document/7758266/en
dc.titleA study of the incubation of microbead agglutination assays in a microfluidic systemen
dc.typeConference Paperen
dc.contributor.departmentKing Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabiaen
dc.identifier.journal2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)en
dc.contributor.institutionUniversity of British Columbia (UBC). Kelenowa, Canadaen
kaust.authorCastro, Daviden
kaust.authorConchouso Gonzalez, Daviden
kaust.authorCarreno, Armando Arpys Arevaloen
kaust.authorFoulds, Ian G.en
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