Label-free detection of sex determining region Y (SRY) via capacitive biosensor

Handle URI:
http://hdl.handle.net/10754/622421
Title:
Label-free detection of sex determining region Y (SRY) via capacitive biosensor
Authors:
Sivashankar, Shilpa; Sapsanis, Christos ( 0000-0003-3875-5110 ) ; Agambayev, Sumeyra; Buttner, Ulrich; Salama, Khaled N. ( 0000-0001-7742-1282 )
Abstract:
In this work, we present for the first time, the use of a simple fractal capacitive biosensor for the quantification and detection of sex-determining region Y (SRY) genes. This section of genetic code, which is found on the Y chromosome, finds importance for study as it causes fetuses to develop characteristics of male sex-like gonads when a mutation occurs. It is also an important genetic code in men, and disorders involving the SRY gene can cause infertility and sexual malfunction that lead to a variety of gene mutational disorders. We have therefore designed silicon-based, label-free fractal capacitive biosensors to quantify various proteins and genes. We take advantage of a good dielectric material, Parylene C for enhancing the performance of the sensors. We have integrated these sensors with a simple microchannel for easy handling of fluids on the detection area. The read-out value of an Agilent LCR meter used to measure capacitance of the sensor at a frequency of 1 MHz determined gene specificity and gene quantification. These data revealed that the capacitance measurement of the capacitive biosensor for the SRY gene depended on both the target and the concentration of DNA. The experimental outcomes in the present study can be used to detect DNA and its variations in crucial fields that have a great impact on our daily lives, such as clinical and veterinary diagnostics, industrial and environmental testing and forensic sciences.
KAUST Department:
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Electrical Engineering Program; Advanced Nanofabrication and Thin Film Core Lab
Citation:
Sivashankar S, Sapsanis C, Agambayev S, Buttner U, Salama KN (2016) Label-free detection of sex determining region Y (SRY) via capacitive biosensor. 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). Available: http://dx.doi.org/10.1109/EMBC.2016.7591690.
Publisher:
Institute of Electrical and Electronics Engineers (IEEE)
Journal:
2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
Issue Date:
20-Oct-2016
DOI:
10.1109/EMBC.2016.7591690
Type:
Conference Paper
Additional Links:
http://ieeexplore.ieee.org/document/7591690/
Appears in Collections:
Conference Papers; Advanced Nanofabrication, Imaging and Characterization Core Lab; Electrical Engineering Program; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorSivashankar, Shilpaen
dc.contributor.authorSapsanis, Christosen
dc.contributor.authorAgambayev, Sumeyraen
dc.contributor.authorButtner, Ulrichen
dc.contributor.authorSalama, Khaled N.en
dc.date.accessioned2017-01-02T09:28:30Z-
dc.date.available2017-01-02T09:28:30Z-
dc.date.issued2016-10-20en
dc.identifier.citationSivashankar S, Sapsanis C, Agambayev S, Buttner U, Salama KN (2016) Label-free detection of sex determining region Y (SRY) via capacitive biosensor. 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). Available: http://dx.doi.org/10.1109/EMBC.2016.7591690.en
dc.identifier.doi10.1109/EMBC.2016.7591690en
dc.identifier.urihttp://hdl.handle.net/10754/622421-
dc.description.abstractIn this work, we present for the first time, the use of a simple fractal capacitive biosensor for the quantification and detection of sex-determining region Y (SRY) genes. This section of genetic code, which is found on the Y chromosome, finds importance for study as it causes fetuses to develop characteristics of male sex-like gonads when a mutation occurs. It is also an important genetic code in men, and disorders involving the SRY gene can cause infertility and sexual malfunction that lead to a variety of gene mutational disorders. We have therefore designed silicon-based, label-free fractal capacitive biosensors to quantify various proteins and genes. We take advantage of a good dielectric material, Parylene C for enhancing the performance of the sensors. We have integrated these sensors with a simple microchannel for easy handling of fluids on the detection area. The read-out value of an Agilent LCR meter used to measure capacitance of the sensor at a frequency of 1 MHz determined gene specificity and gene quantification. These data revealed that the capacitance measurement of the capacitive biosensor for the SRY gene depended on both the target and the concentration of DNA. The experimental outcomes in the present study can be used to detect DNA and its variations in crucial fields that have a great impact on our daily lives, such as clinical and veterinary diagnostics, industrial and environmental testing and forensic sciences.en
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en
dc.relation.urlhttp://ieeexplore.ieee.org/document/7591690/en
dc.titleLabel-free detection of sex determining region Y (SRY) via capacitive biosensoren
dc.typeConference Paperen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentElectrical Engineering Programen
dc.contributor.departmentAdvanced Nanofabrication and Thin Film Core Laben
dc.identifier.journal2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)en
dc.contributor.institutionJohn Hopkins University USAen
kaust.authorSivashankar, Shilpaen
kaust.authorSapsanis, Christosen
kaust.authorAgambayev, Sumeyraen
kaust.authorButtner, Ulrichen
kaust.authorSalama, Khaled N.en
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