Impact of acclimation methods on microbial communities and performance of anaerobic fluidized bed membrane bioreactors

Handle URI:
http://hdl.handle.net/10754/622417
Title:
Impact of acclimation methods on microbial communities and performance of anaerobic fluidized bed membrane bioreactors
Authors:
Labarge, Nicole; Ye, Yaoli; Kim, Kyoung Yeol; Yilmazel, Yasemin Dilsad; Saikaly, Pascal ( 0000-0001-7678-3986 ) ; Hong, Pei-Ying ( 0000-0002-4474-6600 ) ; Logan, Bruce E.
Abstract:
An anaerobic fluidized bed membrane bioreactor (AFMBR) is a new and effective method for energy-efficient treatment of low strength wastewater, but the factors that affect performance are not well known. Different inocula and acclimation methods of the granular activated carbon (GAC) used in the reactor were examined here to determine their impact on chemical oxygen demand (COD) removal and microbial community composition of domestic wastewater-fed AFMBRs. AFMBRs inoculated with anaerobic digester sludge (D) or domestic wastewater (W) and fed domestic wastewater, or inoculated with a microbiologically diverse anaerobic bog sediment and acclimated using methanol (M), all produced the same COD removal of 63 ± 12% using a diluted wastewater feed (100 ± 21 mg L−1 COD). However, an AFMBR with GAC inoculated with anaerobic digester sludge and acclimated using acetate (A) showed significantly increased wastewater COD removal to 84 ± 6%. In addition, feeding the AFMBR with the M-acclimated GAC with an acetate medium for one week subsequently increased COD removal to 70 ± 6%. Microbial communities enriched on the GAC included Geobacter, sulfur-reducing bacteria, Syntrophaceae, and Chlorobiaceae, with reactor A having the highest relative abundance of Geobacter. These results showed that acetate was the most useful substrate for acclimation of GAC communities, and GAC harbors unique communities relative to those in the AFMBR influent and recirculated solution.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
LaBarge N, Ye Y, Kim K-Y, Yilmazel YD, Saikaly PE, et al. (2016) Impact of acclimation methods on microbial communities and performance of anaerobic fluidized bed membrane bioreactors. Environ Sci: Water Res Technol 2: 1041–1048. Available: http://dx.doi.org/10.1039/c6ew00237d.
Publisher:
Royal Society of Chemistry (RSC)
Journal:
Environ. Sci.: Water Res. Technol.
KAUST Grant Number:
OSR-2015-SEED-2450-01
Issue Date:
17-Oct-2016
DOI:
10.1039/c6ew00237d
Type:
Article
ISSN:
2053-1400; 2053-1419
Sponsors:
The authors would like to thank Dr. Hiroyuki Kashima for help with community analysis. This work was supported by Strategic Environmental Research and Development Program (SERDP), and Award OSR-2015-SEED-2450-01 from the King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://pubs.rsc.org/en/Content/ArticleLanding/2016/EW/C6EW00237D
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLabarge, Nicoleen
dc.contributor.authorYe, Yaolien
dc.contributor.authorKim, Kyoung Yeolen
dc.contributor.authorYilmazel, Yasemin Dilsaden
dc.contributor.authorSaikaly, Pascalen
dc.contributor.authorHong, Pei-Yingen
dc.contributor.authorLogan, Bruce E.en
dc.date.accessioned2017-01-02T09:28:29Z-
dc.date.available2017-01-02T09:28:29Z-
dc.date.issued2016-10-17en
dc.identifier.citationLaBarge N, Ye Y, Kim K-Y, Yilmazel YD, Saikaly PE, et al. (2016) Impact of acclimation methods on microbial communities and performance of anaerobic fluidized bed membrane bioreactors. Environ Sci: Water Res Technol 2: 1041–1048. Available: http://dx.doi.org/10.1039/c6ew00237d.en
dc.identifier.issn2053-1400en
dc.identifier.issn2053-1419en
dc.identifier.doi10.1039/c6ew00237den
dc.identifier.urihttp://hdl.handle.net/10754/622417-
dc.description.abstractAn anaerobic fluidized bed membrane bioreactor (AFMBR) is a new and effective method for energy-efficient treatment of low strength wastewater, but the factors that affect performance are not well known. Different inocula and acclimation methods of the granular activated carbon (GAC) used in the reactor were examined here to determine their impact on chemical oxygen demand (COD) removal and microbial community composition of domestic wastewater-fed AFMBRs. AFMBRs inoculated with anaerobic digester sludge (D) or domestic wastewater (W) and fed domestic wastewater, or inoculated with a microbiologically diverse anaerobic bog sediment and acclimated using methanol (M), all produced the same COD removal of 63 ± 12% using a diluted wastewater feed (100 ± 21 mg L−1 COD). However, an AFMBR with GAC inoculated with anaerobic digester sludge and acclimated using acetate (A) showed significantly increased wastewater COD removal to 84 ± 6%. In addition, feeding the AFMBR with the M-acclimated GAC with an acetate medium for one week subsequently increased COD removal to 70 ± 6%. Microbial communities enriched on the GAC included Geobacter, sulfur-reducing bacteria, Syntrophaceae, and Chlorobiaceae, with reactor A having the highest relative abundance of Geobacter. These results showed that acetate was the most useful substrate for acclimation of GAC communities, and GAC harbors unique communities relative to those in the AFMBR influent and recirculated solution.en
dc.description.sponsorshipThe authors would like to thank Dr. Hiroyuki Kashima for help with community analysis. This work was supported by Strategic Environmental Research and Development Program (SERDP), and Award OSR-2015-SEED-2450-01 from the King Abdullah University of Science and Technology (KAUST).en
dc.publisherRoyal Society of Chemistry (RSC)en
dc.relation.urlhttp://pubs.rsc.org/en/Content/ArticleLanding/2016/EW/C6EW00237Den
dc.titleImpact of acclimation methods on microbial communities and performance of anaerobic fluidized bed membrane bioreactorsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalEnviron. Sci.: Water Res. Technol.en
dc.contributor.institutionDepartment of Civil and Environmental Engineering, The Pennsylvania State University, University Park, USAen
kaust.authorSaikaly, Pascalen
kaust.authorHong, Pei-Yingen
kaust.grant.numberOSR-2015-SEED-2450-01en
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