Evidence for a role of viruses in the thermal sensitivity of coral photosymbionts

Handle URI:
http://hdl.handle.net/10754/622401
Title:
Evidence for a role of viruses in the thermal sensitivity of coral photosymbionts
Authors:
Levin, Rachel Ashley; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Weynberg, Karen Dawn; Oppen, Madeleine Josephine Henriette van
Abstract:
Symbiodinium, the dinoflagellate photosymbiont of corals, is posited to become more susceptible to viral infections when heat-stressed. To investigate this hypothesis, we mined transcriptome data of a thermosensitive and a thermotolerant type C1 Symbiodinium population at ambient (27 °C) and elevated (32°C) temperatures. We uncovered hundreds of transcripts from nucleocytoplasmic large double-stranded DNA viruses (NCLDVs) and the genome of a novel positive-sense single-stranded RNA virus (+ssRNAV). In the transcriptome of the thermosensitive population only, +ssRNAV transcripts had remarkable expression levels in the top 0.03% of all transcripts at 27 °C, but at 32 °C, expression levels of +ssRNAV transcripts decreased, while expression levels of anti-viral transcripts increased. In both transcriptomes, expression of NCLDV transcripts increased at 32 °C, but thermal induction of NCLDV transcripts involved in DNA manipulation was restricted to the thermosensitive population. Our findings reveal that viruses infecting Symbiodinium are affected by heat stress and may contribute to Symbiodinium thermal sensitivity.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Levin RA, Voolstra CR, Weynberg KD, van Oppen MJH (2016) Evidence for a role of viruses in the thermal sensitivity of coral photosymbionts. The ISME Journal. Available: http://dx.doi.org/10.1038/ismej.2016.154.
Publisher:
Springer Nature
Journal:
The ISME Journal
Issue Date:
2-Dec-2016
DOI:
10.1038/ismej.2016.154
Type:
Article
ISSN:
1751-7362; 1751-7370
Sponsors:
The Australian Institute of Marine Science supplied the Symbiodinium strains (aims-aten-C1-WSY, aims-aten-C1-MI) used in this study. Yi Jin Liew provided assistance with the bit score analysis. Rhys T Graham provided technical support for MATLAB. The Centre for Marine Bio-Innovation at the University of New South Wales, King Abddullah University of Science and Technology (KAUST), The Joyce W Vickery Scientific Research Fund Grant awarded to Rachel A Levin from the Linnean Society of New South Wales, and Future Fellowship No FT100100088 awarded to Madeleine JH van Oppen from the Australian Research Council contributed financial support. Raw, processed and annotated sequencing data are available through NCBI GEO (accession: GSE77911). The novel +ssRNAV genome (TR74740|c13_g1_i1) and highly related, partial +ssRNAV genome (TR74740|c13_g1_i2) discovered in this study have been deposited in GenBank (accession nos: KX538960 and KX787934, poly(A) tails were trimmed from RNA-Seq reads prior to de novo assembly of transcripts).
Additional Links:
http://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej2016154a.html
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLevin, Rachel Ashleyen
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorWeynberg, Karen Dawnen
dc.contributor.authorOppen, Madeleine Josephine Henriette vanen
dc.date.accessioned2017-01-02T09:28:28Z-
dc.date.available2017-01-02T09:28:28Z-
dc.date.issued2016-12-02en
dc.identifier.citationLevin RA, Voolstra CR, Weynberg KD, van Oppen MJH (2016) Evidence for a role of viruses in the thermal sensitivity of coral photosymbionts. The ISME Journal. Available: http://dx.doi.org/10.1038/ismej.2016.154.en
dc.identifier.issn1751-7362en
dc.identifier.issn1751-7370en
dc.identifier.doi10.1038/ismej.2016.154en
dc.identifier.urihttp://hdl.handle.net/10754/622401-
dc.description.abstractSymbiodinium, the dinoflagellate photosymbiont of corals, is posited to become more susceptible to viral infections when heat-stressed. To investigate this hypothesis, we mined transcriptome data of a thermosensitive and a thermotolerant type C1 Symbiodinium population at ambient (27 °C) and elevated (32°C) temperatures. We uncovered hundreds of transcripts from nucleocytoplasmic large double-stranded DNA viruses (NCLDVs) and the genome of a novel positive-sense single-stranded RNA virus (+ssRNAV). In the transcriptome of the thermosensitive population only, +ssRNAV transcripts had remarkable expression levels in the top 0.03% of all transcripts at 27 °C, but at 32 °C, expression levels of +ssRNAV transcripts decreased, while expression levels of anti-viral transcripts increased. In both transcriptomes, expression of NCLDV transcripts increased at 32 °C, but thermal induction of NCLDV transcripts involved in DNA manipulation was restricted to the thermosensitive population. Our findings reveal that viruses infecting Symbiodinium are affected by heat stress and may contribute to Symbiodinium thermal sensitivity.en
dc.description.sponsorshipThe Australian Institute of Marine Science supplied the Symbiodinium strains (aims-aten-C1-WSY, aims-aten-C1-MI) used in this study. Yi Jin Liew provided assistance with the bit score analysis. Rhys T Graham provided technical support for MATLAB. The Centre for Marine Bio-Innovation at the University of New South Wales, King Abddullah University of Science and Technology (KAUST), The Joyce W Vickery Scientific Research Fund Grant awarded to Rachel A Levin from the Linnean Society of New South Wales, and Future Fellowship No FT100100088 awarded to Madeleine JH van Oppen from the Australian Research Council contributed financial support. Raw, processed and annotated sequencing data are available through NCBI GEO (accession: GSE77911). The novel +ssRNAV genome (TR74740|c13_g1_i1) and highly related, partial +ssRNAV genome (TR74740|c13_g1_i2) discovered in this study have been deposited in GenBank (accession nos: KX538960 and KX787934, poly(A) tails were trimmed from RNA-Seq reads prior to de novo assembly of transcripts).en
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej2016154a.htmlen
dc.titleEvidence for a role of viruses in the thermal sensitivity of coral photosymbiontsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalThe ISME Journalen
dc.contributor.institutionCentre for Marine Bio-Innovation, The University of New South Wales (UNSW), Sydney, NSW, Australiaen
dc.contributor.institutionSchool of Biological, Earth and Environmental Sciences, The University of New South Wales, Sydney, NSW, Australiaen
dc.contributor.institutionClimate Change Cluster, University of Technology Sydney, Sydney, NSW, Australiaen
dc.contributor.institutionAustralian Institute of Marine Science, Townsville, QLD, Australiaen
dc.contributor.institutionSchool of BioSciences, The University of Melbourne, Parkville, VIC, Australiaen
kaust.authorVoolstra, Christian R.en
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