Is metal contamination responsible for increasing aneuploidy levels in the Manila clam Ruditapes philippinarum?

Handle URI:
http://hdl.handle.net/10754/622303
Title:
Is metal contamination responsible for increasing aneuploidy levels in the Manila clam Ruditapes philippinarum?
Authors:
Piló, D.; Carvalho, Susana ( 0000-0003-1300-1953 ) ; Pereira, P.; Gaspar, M.B.; Leitão, A.
Abstract:
The present study assessed the metal genotoxicity potential at chromosome-level in the bivalve Ruditapes philippinarum collected along different areas of the Tagus estuary. Higher levels of aneuploidy on gill cells were detected at the most sediment contaminated area both in May (31.7%) and October (36.0%) when compared to a less contaminated area over the same periods (20.3% and 29.0% respectively). Interestingly, metal bioaccumulation in gills was higher in the specimens collected at the least contaminated area with the exception of Pb. Indeed, the multivariate analysis revealed a stronger relation between aneuploidy and sediment contamination than between aneuploidy and the bioaccumulation of the metals. The temporal and spatial inconsistency found for the bioaccumulation of metals in R. philippinarum and the positive correlation between sediment contamination and aneuploidy at the most contaminated area suggest that these chromosome-level effects might be due to chronic metal contamination occurring in the Tagus estuary, rather than a direct result of the temporal variation of bioavailable contaminants. The vertical transmission phenomenon of bivalve aneuploidy levels may then be perpetuating those levels on clams from the most contaminated area. The present results shed light about the effect of metal toxicity at the chromosome-level in species inhabiting chronic contaminated areas and highlight the use of aneuploidy as an effective tool to identify persistent contamination in worldwide transitional waters.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Piló D, Carvalho S, Pereira P, Gaspar MB, Leitão A (2017) Is metal contamination responsible for increasing aneuploidy levels in the Manila clam Ruditapes philippinarum? Science of The Total Environment 577: 340–348. Available: http://dx.doi.org/10.1016/j.scitotenv.2016.10.193.
Publisher:
Elsevier BV
Journal:
Science of The Total Environment
Issue Date:
3-Nov-2016
DOI:
10.1016/j.scitotenv.2016.10.193
Type:
Article
ISSN:
0048-9697
Sponsors:
This study was funded by the “Fundação para a Ciência e Tecnologia” (FCT) project “ECOAPPROACH” (PTDC/AAC-AMB/121037/2010). The authors wish to thank to Fábio Pereira for his valuable help in the sampling surveys and to Dr. Radhouan Ben-Hamadou for his help in the sampling design. We are also grateful to the “Estrutura de Missão para a Extensão da Plataforma Continental” (EMEPC) for the boat used during this study, and to Adolfo Lobo and Luís Bernardes for sampling assistance. A special thank are due to Margarete Matias for her assistance in the microscope slide observation. We are also indebted to Dr. Joana Raimundo, Olinda Araújo and Ana Carriço for their help in the laboratory work. D.P. benefit from a grant from the above mentioned project and P.P. benefit from a Post-doctoral grant (SFRH/BPD/69563/2010) awarded by the FCT. The authors would also like to thank Dr. Joanne Ellis for proofreading the manuscript as well as the editor and reviewers for their constructive comments that contributed to substantially improve an earlier version of the manuscript.
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0048969716323828
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorPiló, D.en
dc.contributor.authorCarvalho, Susanaen
dc.contributor.authorPereira, P.en
dc.contributor.authorGaspar, M.B.en
dc.contributor.authorLeitão, A.en
dc.date.accessioned2017-01-02T09:08:24Z-
dc.date.available2017-01-02T09:08:24Z-
dc.date.issued2016-11-03en
dc.identifier.citationPiló D, Carvalho S, Pereira P, Gaspar MB, Leitão A (2017) Is metal contamination responsible for increasing aneuploidy levels in the Manila clam Ruditapes philippinarum? Science of The Total Environment 577: 340–348. Available: http://dx.doi.org/10.1016/j.scitotenv.2016.10.193.en
dc.identifier.issn0048-9697en
dc.identifier.doi10.1016/j.scitotenv.2016.10.193en
dc.identifier.urihttp://hdl.handle.net/10754/622303-
dc.description.abstractThe present study assessed the metal genotoxicity potential at chromosome-level in the bivalve Ruditapes philippinarum collected along different areas of the Tagus estuary. Higher levels of aneuploidy on gill cells were detected at the most sediment contaminated area both in May (31.7%) and October (36.0%) when compared to a less contaminated area over the same periods (20.3% and 29.0% respectively). Interestingly, metal bioaccumulation in gills was higher in the specimens collected at the least contaminated area with the exception of Pb. Indeed, the multivariate analysis revealed a stronger relation between aneuploidy and sediment contamination than between aneuploidy and the bioaccumulation of the metals. The temporal and spatial inconsistency found for the bioaccumulation of metals in R. philippinarum and the positive correlation between sediment contamination and aneuploidy at the most contaminated area suggest that these chromosome-level effects might be due to chronic metal contamination occurring in the Tagus estuary, rather than a direct result of the temporal variation of bioavailable contaminants. The vertical transmission phenomenon of bivalve aneuploidy levels may then be perpetuating those levels on clams from the most contaminated area. The present results shed light about the effect of metal toxicity at the chromosome-level in species inhabiting chronic contaminated areas and highlight the use of aneuploidy as an effective tool to identify persistent contamination in worldwide transitional waters.en
dc.description.sponsorshipThis study was funded by the “Fundação para a Ciência e Tecnologia” (FCT) project “ECOAPPROACH” (PTDC/AAC-AMB/121037/2010). The authors wish to thank to Fábio Pereira for his valuable help in the sampling surveys and to Dr. Radhouan Ben-Hamadou for his help in the sampling design. We are also grateful to the “Estrutura de Missão para a Extensão da Plataforma Continental” (EMEPC) for the boat used during this study, and to Adolfo Lobo and Luís Bernardes for sampling assistance. A special thank are due to Margarete Matias for her assistance in the microscope slide observation. We are also indebted to Dr. Joana Raimundo, Olinda Araújo and Ana Carriço for their help in the laboratory work. D.P. benefit from a grant from the above mentioned project and P.P. benefit from a Post-doctoral grant (SFRH/BPD/69563/2010) awarded by the FCT. The authors would also like to thank Dr. Joanne Ellis for proofreading the manuscript as well as the editor and reviewers for their constructive comments that contributed to substantially improve an earlier version of the manuscript.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0048969716323828en
dc.subjectEstuaryen
dc.subjectMetal contaminationen
dc.subjectRuditapes philippinarumen
dc.subjectChromosomeen
dc.subjectAneuploidyen
dc.subjectVertical transmissionen
dc.titleIs metal contamination responsible for increasing aneuploidy levels in the Manila clam Ruditapes philippinarum?en
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalScience of The Total Environmenten
dc.contributor.institutionPortuguese Institute for the Ocean and Atmosphere (IPMA), Avenida 5 de Outubro, 8700-305 Olhão, Portugalen
dc.contributor.institutionDepartment of Biology, Faculty of Marine and Environment Sciences, University of Cadiz, Campus de Excelencia International del Mar (CEIMAR), Avda. República Saharaui S/N Puerto Real, 11510 Puerto Real, Cadiz, Spainen
dc.contributor.institutionPortuguese Institute for the Ocean and Atmosphere (IPMA), Av. Brasília, 1449-006 Lisbon, Portugalen
dc.contributor.institutionCentre for Environmental and Marine Studies and Department of Biology (CESAM), University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugalen
dc.contributor.institutionCentre of Marine Sciences (CCMAR), University of Algarve (UAlg), Campus de Gambelas, 8005-139 Faro, Portugalen
dc.contributor.institutionEnvironmental Science Center (ESC), Qatar University, P.O. Box 2713, Doha, Qataren
kaust.authorCarvalho, Susanaen
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