Assessment of the performance of SMFCs in the bioremediation of PAHs in contaminated marine sediments under different redox conditions and analysis of the associated microbial communities

Handle URI:
http://hdl.handle.net/10754/622275
Title:
Assessment of the performance of SMFCs in the bioremediation of PAHs in contaminated marine sediments under different redox conditions and analysis of the associated microbial communities
Authors:
Hamdan, Hamdan Z.; Salam, Darine A.; Rao, Hari Ananda ( 0000-0002-8916-1809 ) ; Semerjian, Lucy; Saikaly, Pascal ( 0000-0001-7678-3986 )
Abstract:
The biodegradation of naphthalene, 2-methylnaphthalene and phenanthrene was evaluated in marine sediment microbial fuel cells (SMFCs) under different biodegradation conditions, including sulfate reduction as a major biodegradation pathway, employment of anode as terminal electron acceptor (TEA) under inhibited sulfate reducing bacteria activity, and combined sulfate and anode usage as electron acceptors. A significant removal of naphthalene and 2-methylnaphthalene was observed at early stages of incubation in all treatments and was attributed to their high volatility. In the case of phenanthrene, a significant removal (93.83 ± 1.68%) was measured in the closed circuit SMFCs with the anode acting as the main TEA and under combined anode and sulfate reduction conditions (88.51 ± 1.3%). A much lower removal (40.37 ± 3.24%) was achieved in the open circuit SMFCs operating with sulfate reduction as a major biodegradation pathway. Analysis of the anodic bacterial community using 16S rRNA gene pyrosequencing revealed the enrichment of genera with potential exoelectrogenic capability, namely Geoalkalibacter and Desulfuromonas, on the anode of the closed circuit SMFCs under inhibited SRB activity, while they were not detected on the anode of open circuit SMFCs. These results demonstrate the role of the anode in enhancing PAHs biodegradation in contaminated marine sediments and suggest a higher system efficiency in the absence of competition between microbial redox processes (under SRB inhibition), namely due to the anode enrichment with exoelectrogenic bacteria, which is a more energetically favorable mechanism for PAHs oxidation than sulfate.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Hamdan HZ, Salam DA, Hari AR, Semerjian L, Saikaly P (2017) Assessment of the performance of SMFCs in the bioremediation of PAHs in contaminated marine sediments under different redox conditions and analysis of the associated microbial communities. Science of The Total Environment 575: 1453–1461. Available: http://dx.doi.org/10.1016/j.scitotenv.2016.09.232.
Publisher:
Elsevier BV
Journal:
Science of The Total Environment
Issue Date:
9-Oct-2016
DOI:
10.1016/j.scitotenv.2016.09.232
Type:
Article
ISSN:
0048-9697
Sponsors:
American University of Beirut[21739]
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorHamdan, Hamdan Z.en
dc.contributor.authorSalam, Darine A.en
dc.contributor.authorRao, Hari Anandaen
dc.contributor.authorSemerjian, Lucyen
dc.contributor.authorSaikaly, Pascalen
dc.date.accessioned2017-01-02T09:08:23Z-
dc.date.available2017-01-02T09:08:23Z-
dc.date.issued2016-10-09en
dc.identifier.citationHamdan HZ, Salam DA, Hari AR, Semerjian L, Saikaly P (2017) Assessment of the performance of SMFCs in the bioremediation of PAHs in contaminated marine sediments under different redox conditions and analysis of the associated microbial communities. Science of The Total Environment 575: 1453–1461. Available: http://dx.doi.org/10.1016/j.scitotenv.2016.09.232.en
dc.identifier.issn0048-9697en
dc.identifier.doi10.1016/j.scitotenv.2016.09.232en
dc.identifier.urihttp://hdl.handle.net/10754/622275-
dc.description.abstractThe biodegradation of naphthalene, 2-methylnaphthalene and phenanthrene was evaluated in marine sediment microbial fuel cells (SMFCs) under different biodegradation conditions, including sulfate reduction as a major biodegradation pathway, employment of anode as terminal electron acceptor (TEA) under inhibited sulfate reducing bacteria activity, and combined sulfate and anode usage as electron acceptors. A significant removal of naphthalene and 2-methylnaphthalene was observed at early stages of incubation in all treatments and was attributed to their high volatility. In the case of phenanthrene, a significant removal (93.83 ± 1.68%) was measured in the closed circuit SMFCs with the anode acting as the main TEA and under combined anode and sulfate reduction conditions (88.51 ± 1.3%). A much lower removal (40.37 ± 3.24%) was achieved in the open circuit SMFCs operating with sulfate reduction as a major biodegradation pathway. Analysis of the anodic bacterial community using 16S rRNA gene pyrosequencing revealed the enrichment of genera with potential exoelectrogenic capability, namely Geoalkalibacter and Desulfuromonas, on the anode of the closed circuit SMFCs under inhibited SRB activity, while they were not detected on the anode of open circuit SMFCs. These results demonstrate the role of the anode in enhancing PAHs biodegradation in contaminated marine sediments and suggest a higher system efficiency in the absence of competition between microbial redox processes (under SRB inhibition), namely due to the anode enrichment with exoelectrogenic bacteria, which is a more energetically favorable mechanism for PAHs oxidation than sulfate.en
dc.description.sponsorshipAmerican University of Beirut[21739]en
dc.publisherElsevier BVen
dc.subjectBacterial communityen
dc.subjectBiodegradationen
dc.subjectPolycyclic aromatic hydrocarbons (PAHs)en
dc.subjectSediment microbial fuel cell (SMFC)en
dc.subjectTerminal electron acceptor (TEA)en
dc.titleAssessment of the performance of SMFCs in the bioremediation of PAHs in contaminated marine sediments under different redox conditions and analysis of the associated microbial communitiesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalScience of The Total Environmenten
dc.contributor.institutionDepartment of Civil and Environmental Engineering, Faculty of Engineering and Architecture, American University of Beirut, Beirut, Lebanonen
dc.contributor.institutionDepartment of Environmental Health Sciences, College of Health Sciences, University of Sharjah, Sharjah, United Arab Emiratesen
kaust.authorRao, Hari Anandaen
kaust.authorSaikaly, Pascalen
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