Comparative Assessment of Mediterranean Gorgonian-Associated Microbial Communities Reveals Conserved Core and Locally Variant Bacteria

Handle URI:
http://hdl.handle.net/10754/622184
Title:
Comparative Assessment of Mediterranean Gorgonian-Associated Microbial Communities Reveals Conserved Core and Locally Variant Bacteria
Authors:
van de Water, Jeroen A J M; Melkonian, Rémy; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Junca, Howard; Beraud, Eric; Allemand, Denis; Ferrier-Pagès, Christine
Abstract:
Gorgonians are key habitat-forming species of Mediterranean benthic communities, but their populations have suffered from mass mortality events linked to high summer seawater temperatures and microbial disease. However, our knowledge on the diversity, dynamics and function of gorgonian-associated microbial communities is limited. Here, we analysed the spatial variability of the microbiomes of five sympatric gorgonian species (Eunicella singularis, Eunicella cavolini, Eunicella verrucosa, Leptogorgia sarmentosa and Paramuricea clavata), collected from the Mediterranean Sea over a scale of ∼1100 km, using next-generation amplicon sequencing of the 16S rRNA gene. The microbiomes of all gorgonian species were generally dominated by members of the genus Endozoicomonas, which were at very low abundance in the surrounding seawater. Although the composition of the core microbiome (operational taxonomic units consistently present in a species) was found to be unique for each host species, significant overlap was observed. These spatially consistent associations between gorgonians and their core bacteria suggest intricate symbiotic relationships and regulation of the microbiome composition by the host. At the same time, local variations in microbiome composition were observed. Functional predictive profiling indicated that these differences could be attributed to seawater pollution. Taken together, our data indicate that gorgonian-associated microbiomes are composed of spatially conserved bacteria (core microbiome members) and locally variant members, and that local pollution may influence these local associations, potentially impacting gorgonian health.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Van de Water JAJM, Melkonian R, Voolstra CR, Junca H, Beraud E, et al. (2016) Comparative Assessment of Mediterranean Gorgonian-Associated Microbial Communities Reveals Conserved Core and Locally Variant Bacteria. Microbial Ecology. Available: http://dx.doi.org/10.1007/s00248-016-0858-x.
Publisher:
Springer Nature
Journal:
Microbial Ecology
Issue Date:
10-Oct-2016
DOI:
10.1007/s00248-016-0858-x
Type:
Article
ISSN:
0095-3628; 1432-184X
Sponsors:
King Abdullah University of Science and Technology; Fondation Paul Hamel
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorvan de Water, Jeroen A J Men
dc.contributor.authorMelkonian, Rémyen
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorJunca, Howarden
dc.contributor.authorBeraud, Ericen
dc.contributor.authorAllemand, Denisen
dc.contributor.authorFerrier-Pagès, Christineen
dc.date.accessioned2017-01-02T08:42:36Z-
dc.date.available2017-01-02T08:42:36Z-
dc.date.issued2016-10-10en
dc.identifier.citationVan de Water JAJM, Melkonian R, Voolstra CR, Junca H, Beraud E, et al. (2016) Comparative Assessment of Mediterranean Gorgonian-Associated Microbial Communities Reveals Conserved Core and Locally Variant Bacteria. Microbial Ecology. Available: http://dx.doi.org/10.1007/s00248-016-0858-x.en
dc.identifier.issn0095-3628en
dc.identifier.issn1432-184Xen
dc.identifier.doi10.1007/s00248-016-0858-xen
dc.identifier.urihttp://hdl.handle.net/10754/622184-
dc.description.abstractGorgonians are key habitat-forming species of Mediterranean benthic communities, but their populations have suffered from mass mortality events linked to high summer seawater temperatures and microbial disease. However, our knowledge on the diversity, dynamics and function of gorgonian-associated microbial communities is limited. Here, we analysed the spatial variability of the microbiomes of five sympatric gorgonian species (Eunicella singularis, Eunicella cavolini, Eunicella verrucosa, Leptogorgia sarmentosa and Paramuricea clavata), collected from the Mediterranean Sea over a scale of ∼1100 km, using next-generation amplicon sequencing of the 16S rRNA gene. The microbiomes of all gorgonian species were generally dominated by members of the genus Endozoicomonas, which were at very low abundance in the surrounding seawater. Although the composition of the core microbiome (operational taxonomic units consistently present in a species) was found to be unique for each host species, significant overlap was observed. These spatially consistent associations between gorgonians and their core bacteria suggest intricate symbiotic relationships and regulation of the microbiome composition by the host. At the same time, local variations in microbiome composition were observed. Functional predictive profiling indicated that these differences could be attributed to seawater pollution. Taken together, our data indicate that gorgonian-associated microbiomes are composed of spatially conserved bacteria (core microbiome members) and locally variant members, and that local pollution may influence these local associations, potentially impacting gorgonian health.en
dc.description.sponsorshipKing Abdullah University of Science and Technologyen
dc.description.sponsorshipFondation Paul Hamelen
dc.publisherSpringer Natureen
dc.subject16S rRNA geneen
dc.subjectBacterial communityen
dc.subjectCoralen
dc.subjectEndozoicomonasen
dc.subjectEvolutionen
dc.subjectGorgonianen
dc.subjectHolobionten
dc.titleComparative Assessment of Mediterranean Gorgonian-Associated Microbial Communities Reveals Conserved Core and Locally Variant Bacteriaen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalMicrobial Ecologyen
dc.contributor.institutionCentre Scientifique de Monaco, 8 Quai Antoine 1, Monaco, MC 98000, Monacoen
dc.contributor.institutionMicrobiomas Foundation - Division of Ecogenomics & Holobionts, Chia, Colombiaen
kaust.authorVoolstra, Christian R.en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.