Cellular Dynamics Revealed by Digital Holographic Microscopy☆

Handle URI:
http://hdl.handle.net/10754/622179
Title:
Cellular Dynamics Revealed by Digital Holographic Microscopy☆
Authors:
Marquet, P.; Depeursinge, Christian; Jourdain, P.
Abstract:
Digital holographic microscopy (DHM) is a new optical method that provides, without the use of any contrast agent, real-time, three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. They result from the hologram numerical reconstruction process, which permits a sub wavelength calculation of the phase shift, produced on the transmitted wave front, by the optically probed cells, namely the quantitative phase signal (QPS). Specifically, in addition to measurements of cellular surface morphometry and intracellular refractive index (RI), various biophysical cellular parameters including dry mass, absolute volume, membrane fluctuations at the nanoscale and biomechanical properties, transmembrane water permeability as swell as current, can be derived from the QPS. This article presents how quantitative phase DHM (QP-DHM) can explored cell dynamics at the nanoscale with a special attention to both the study of neuronal dynamics and the optical resolution of local neuronal network.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Marquet P, Depeursinge C, Jourdain P (2017) Cellular Dynamics Revealed by Digital Holographic Microscopy☆. Reference Module in Neuroscience and Biobehavioral Psychology. Available: http://dx.doi.org/10.1016/B978-0-12-809324-5.02426-3.
Publisher:
Elsevier BV
Journal:
Reference Module in Neuroscience and Biobehavioral Psychology
Issue Date:
22-Nov-2016
DOI:
10.1016/B978-0-12-809324-5.02426-3
Type:
Book Chapter
Additional Links:
http://www.sciencedirect.com/science/article/pii/B9780128093245024263
Appears in Collections:
Book Chapters; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMarquet, P.en
dc.contributor.authorDepeursinge, Christianen
dc.contributor.authorJourdain, P.en
dc.date.accessioned2017-01-02T08:42:36Z-
dc.date.available2017-01-02T08:42:36Z-
dc.date.issued2016-11-22en
dc.identifier.citationMarquet P, Depeursinge C, Jourdain P (2017) Cellular Dynamics Revealed by Digital Holographic Microscopy☆. Reference Module in Neuroscience and Biobehavioral Psychology. Available: http://dx.doi.org/10.1016/B978-0-12-809324-5.02426-3.en
dc.identifier.doi10.1016/B978-0-12-809324-5.02426-3en
dc.identifier.urihttp://hdl.handle.net/10754/622179-
dc.description.abstractDigital holographic microscopy (DHM) is a new optical method that provides, without the use of any contrast agent, real-time, three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. They result from the hologram numerical reconstruction process, which permits a sub wavelength calculation of the phase shift, produced on the transmitted wave front, by the optically probed cells, namely the quantitative phase signal (QPS). Specifically, in addition to measurements of cellular surface morphometry and intracellular refractive index (RI), various biophysical cellular parameters including dry mass, absolute volume, membrane fluctuations at the nanoscale and biomechanical properties, transmembrane water permeability as swell as current, can be derived from the QPS. This article presents how quantitative phase DHM (QP-DHM) can explored cell dynamics at the nanoscale with a special attention to both the study of neuronal dynamics and the optical resolution of local neuronal network.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/B9780128093245024263en
dc.subjectCellular dynamicsen
dc.subjectCotransportersen
dc.subjectDigital holographic microscopyen
dc.subjectExcitotoxicityen
dc.subjectGlutamateen
dc.subjectInterferometryen
dc.subjectMicroscopyen
dc.subjectNeuron cell cultureen
dc.subjectOptical imagingen
dc.subjectTransmembrane water movementsen
dc.titleCellular Dynamics Revealed by Digital Holographic Microscopy☆en
dc.typeBook Chapteren
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalReference Module in Neuroscience and Biobehavioral Psychologyen
dc.contributor.institutionUniversitaire Vaudois, Prilly/Lausanne, Switzerlanden
dc.contributor.institutionInstitut Universitaire en Santé Mentale de Québec, Quebec, QC, Canadaen
dc.contributor.institutionUniversité Laval, Quebec, QC, Canadaen
dc.contributor.institutionBrain Mind Institute, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerlanden
kaust.authorDepeursinge, Christianen
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