Acquisition and Analysis of Data from High Concentration Solutions

Handle URI:
http://hdl.handle.net/10754/622170
Title:
Acquisition and Analysis of Data from High Concentration Solutions
Authors:
Besong, Tabot M.D. ( 0000-0002-3572-1428 ) ; Rowe, Arthur J.
Abstract:
The problems associated with ultracentrifugal analysis of macromolecular solutions at high (>10 mg/ml) are reviewed. Especially for the case of solutes which are non-monodisperse, meaningful results are not readily achievable using sedimentation velocity approaches. It is shown however by both simulation and analysis of practical data that using a modified form of an algorithm (INVEQ) published in other contexts, sedimentation equilibrium (SE) profiles can be analysed successfully, enabling topics such as oligomer presence or formation to be defined.To achieve this, it is necessary to employ an approach in which the solution density, which in an SE profile is radius-dependent, is taken into consideration. Simulation suggests that any reasonable level of solute concentration can be analysed.
KAUST Department:
Functional Nanomaterials Lab (FuNL); Physical Sciences and Engineering (PSE) Division
Citation:
Besong TMD, Rowe AJ (2016) Acquisition and Analysis of Data from High Concentration Solutions. Analytical Ultracentrifugation: 499–520. Available: http://dx.doi.org/10.1007/978-4-431-55985-6_25.
Publisher:
Springer Nature
Journal:
Analytical Ultracentrifugation
Issue Date:
13-May-2016
DOI:
10.1007/978-4-431-55985-6_25
Type:
Book Chapter
Appears in Collections:
Physical Sciences and Engineering (PSE) Division; Book Chapters

Full metadata record

DC FieldValue Language
dc.contributor.authorBesong, Tabot M.D.en
dc.contributor.authorRowe, Arthur J.en
dc.date.accessioned2017-01-02T08:42:35Z-
dc.date.available2017-01-02T08:42:35Z-
dc.date.issued2016-05-13en
dc.identifier.citationBesong TMD, Rowe AJ (2016) Acquisition and Analysis of Data from High Concentration Solutions. Analytical Ultracentrifugation: 499–520. Available: http://dx.doi.org/10.1007/978-4-431-55985-6_25.en
dc.identifier.doi10.1007/978-4-431-55985-6_25en
dc.identifier.urihttp://hdl.handle.net/10754/622170-
dc.description.abstractThe problems associated with ultracentrifugal analysis of macromolecular solutions at high (>10 mg/ml) are reviewed. Especially for the case of solutes which are non-monodisperse, meaningful results are not readily achievable using sedimentation velocity approaches. It is shown however by both simulation and analysis of practical data that using a modified form of an algorithm (INVEQ) published in other contexts, sedimentation equilibrium (SE) profiles can be analysed successfully, enabling topics such as oligomer presence or formation to be defined.To achieve this, it is necessary to employ an approach in which the solution density, which in an SE profile is radius-dependent, is taken into consideration. Simulation suggests that any reasonable level of solute concentration can be analysed.en
dc.publisherSpringer Natureen
dc.subjectAnalytical ultracentrifugeen
dc.subjectConcentrated solutionsen
dc.subjectOligomersen
dc.subjectSedimentation velocityen
dc.titleAcquisition and Analysis of Data from High Concentration Solutionsen
dc.typeBook Chapteren
dc.contributor.departmentFunctional Nanomaterials Lab (FuNL)en
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalAnalytical Ultracentrifugationen
dc.contributor.institutionNCMH School of Biosciences, University of Nottingham, Sutton Bonington, United Kingdomen
kaust.authorBesong, Tabot M.D.en
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