Raman on suspended DNA: Novel super-hydrophobic approach for structural studies

Handle URI:
http://hdl.handle.net/10754/622075
Title:
Raman on suspended DNA: Novel super-hydrophobic approach for structural studies
Authors:
Marini, Monica ( 0000-0001-8182-5239 ) ; Allione, Marco ( 0000-0003-0757-9791 ) ; Torre, Bruno; Moretti, Manola; Limongi, Tania; Tirinato, Luca ( 0000-0001-9826-2129 ) ; Giugni, Andrea; Das, Gobind ( 0000-0003-0942-681X ) ; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
The A- and B-form are two of the most common structural conformations of double strand DNA present in nature and they can interchange on the basis of the helices hydration [1,2]. Herein we demonstrate that the use of non-destructive techniques such as Raman spectroscopy coupled with the use of a super-hydrophobic device, allows the clear identification of the DNA hydration state, of the backbone (phosphate + deoxyribose sugar) conformation and of the nucleotides. There is a wide prospect for an increase of knowledge in biomolecules using this combined approach resulting in a significant impact in the study of more complex supramolecular assemblies and of fine chemical variation along the genomic loci undergoing to epigenetic variations.
KAUST Department:
Physical Sciences and Engineering (PSE) Division
Citation:
Marini M, Allione M, Torre B, Moretti M, Limongi T, et al. (2016) Raman on suspended DNA: Novel super-hydrophobic approach for structural studies. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2016.12.016.
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
24-Dec-2016
DOI:
10.1016/j.mee.2016.12.016
Type:
Article
ISSN:
0167-9317
Sponsors:
The authors acknowledge financial support from the KAUST start-up funding and from Ministry of Health, Italy under the projects: Project no.: GR-2010-2320665 and Project no.: GR-2010-2311677.
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0167931716305275
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMarini, Monicaen
dc.contributor.authorAllione, Marcoen
dc.contributor.authorTorre, Brunoen
dc.contributor.authorMoretti, Manolaen
dc.contributor.authorLimongi, Taniaen
dc.contributor.authorTirinato, Lucaen
dc.contributor.authorGiugni, Andreaen
dc.contributor.authorDas, Gobinden
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2016-12-26T13:42:26Z-
dc.date.available2016-12-26T13:42:26Z-
dc.date.issued2016-12-24en
dc.identifier.citationMarini M, Allione M, Torre B, Moretti M, Limongi T, et al. (2016) Raman on suspended DNA: Novel super-hydrophobic approach for structural studies. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2016.12.016.en
dc.identifier.issn0167-9317en
dc.identifier.doi10.1016/j.mee.2016.12.016en
dc.identifier.urihttp://hdl.handle.net/10754/622075-
dc.description.abstractThe A- and B-form are two of the most common structural conformations of double strand DNA present in nature and they can interchange on the basis of the helices hydration [1,2]. Herein we demonstrate that the use of non-destructive techniques such as Raman spectroscopy coupled with the use of a super-hydrophobic device, allows the clear identification of the DNA hydration state, of the backbone (phosphate + deoxyribose sugar) conformation and of the nucleotides. There is a wide prospect for an increase of knowledge in biomolecules using this combined approach resulting in a significant impact in the study of more complex supramolecular assemblies and of fine chemical variation along the genomic loci undergoing to epigenetic variations.en
dc.description.sponsorshipThe authors acknowledge financial support from the KAUST start-up funding and from Ministry of Health, Italy under the projects: Project no.: GR-2010-2320665 and Project no.: GR-2010-2311677.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0167931716305275en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microelectronic Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microelectronic Engineering, 24 December 2016. DOI: 10.1016/j.mee.2016.12.016en
dc.subjectDNAen
dc.subjectA-formen
dc.subjectB-formen
dc.subjectSuper-hydrophobic devicesen
dc.subjectRaman spectroscopyen
dc.titleRaman on suspended DNA: Novel super-hydrophobic approach for structural studiesen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalMicroelectronic Engineeringen
dc.eprint.versionPost-printen
kaust.authorMarini, Monicaen
kaust.authorAllione, Marcoen
kaust.authorTorre, Brunoen
kaust.authorMoretti, Manolaen
kaust.authorLimongi, Taniaen
kaust.authorTirinato, Lucaen
kaust.authorGiugni, Andreaen
kaust.authorDas, Gobinden
kaust.authorDi Fabrizio, Enzo M.en
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