Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater: a case study

Handle URI:
http://hdl.handle.net/10754/622073
Title:
Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater: a case study
Authors:
Harb, Moustapha ( 0000-0002-1566-987X ) ; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
Although membrane bioreactor (MBR) systems provide better removal of pathogens compared to conventional activated sludge processes, they do not achieve total log removal. The present study examines two MBR systems treating municipal wastewater, one a full-scale MBR plant and the other a lab-scale anaerobic MBR. Both of these systems were operated using microfiltration (MF) polymeric membranes. High-throughput sequencing and digital PCR quantification were utilized to monitor the log removal values (LRVs) of associated pathogenic species and their abundance in the MBR effluents. Results showed that specific removal rates vary widely regardless of the system employed. Each of the two MBR effluents’ microbial communities contained genera associated with opportunistic pathogens (e.g., Pseudomonas, Acinetobacter) with a wide range of log reduction values (< 2 to >5.5). Digital PCR further confirmed that these bacterial groups included pathogenic species, in several instances at LRVs different than those for their respective genera. These results were used to evaluate the potential risks associated both with the reuse of the MBR effluents for irrigation purposes and with land application of the activated sludge from the full-scale MBR system.
KAUST Department:
Water Desalination and Reuse Research Center (WDRC)
Citation:
Harb M, Hong P-Y (2016) Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater: a case study. Environmental Science and Pollution Research. Available: http://dx.doi.org/10.1007/s11356-016-8211-y.
Publisher:
Springer Nature
Journal:
Environmental Science and Pollution Research
KAUST Grant Number:
FCC/1/1971-06-01
Issue Date:
24-Dec-2016
DOI:
10.1007/s11356-016-8211-y
Type:
Article
ISSN:
0944-1344; 1614-7499
Sponsors:
The research reported in this publication was supported by the KAUST Center Competitive Funding Program grant (FCC/1/1971-06-01) awarded to P-Y Hong. The authors would like to thank Mr. George Princeton Dunsford for access to the KAUST wastewater treatment plant and Dr. Muhammad Raihan Jumat for providing sampling assistance.
Additional Links:
http://link.springer.com/article/10.1007%2Fs11356-016-8211-y
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorHarb, Moustaphaen
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2016-12-26T13:42:26Z-
dc.date.available2016-12-26T13:42:26Z-
dc.date.issued2016-12-24en
dc.identifier.citationHarb M, Hong P-Y (2016) Molecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater: a case study. Environmental Science and Pollution Research. Available: http://dx.doi.org/10.1007/s11356-016-8211-y.en
dc.identifier.issn0944-1344en
dc.identifier.issn1614-7499en
dc.identifier.doi10.1007/s11356-016-8211-yen
dc.identifier.urihttp://hdl.handle.net/10754/622073-
dc.description.abstractAlthough membrane bioreactor (MBR) systems provide better removal of pathogens compared to conventional activated sludge processes, they do not achieve total log removal. The present study examines two MBR systems treating municipal wastewater, one a full-scale MBR plant and the other a lab-scale anaerobic MBR. Both of these systems were operated using microfiltration (MF) polymeric membranes. High-throughput sequencing and digital PCR quantification were utilized to monitor the log removal values (LRVs) of associated pathogenic species and their abundance in the MBR effluents. Results showed that specific removal rates vary widely regardless of the system employed. Each of the two MBR effluents’ microbial communities contained genera associated with opportunistic pathogens (e.g., Pseudomonas, Acinetobacter) with a wide range of log reduction values (< 2 to >5.5). Digital PCR further confirmed that these bacterial groups included pathogenic species, in several instances at LRVs different than those for their respective genera. These results were used to evaluate the potential risks associated both with the reuse of the MBR effluents for irrigation purposes and with land application of the activated sludge from the full-scale MBR system.en
dc.description.sponsorshipThe research reported in this publication was supported by the KAUST Center Competitive Funding Program grant (FCC/1/1971-06-01) awarded to P-Y Hong. The authors would like to thank Mr. George Princeton Dunsford for access to the KAUST wastewater treatment plant and Dr. Muhammad Raihan Jumat for providing sampling assistance.en
dc.publisherSpringer Natureen
dc.relation.urlhttp://link.springer.com/article/10.1007%2Fs11356-016-8211-yen
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPathogensen
dc.subjectRemoval ratesen
dc.subjectAerobicen
dc.subjectAnaerobicen
dc.subjectReuseen
dc.subjectBioreactoren
dc.subjectWastewateren
dc.titleMolecular-based detection of potentially pathogenic bacteria in membrane bioreactor (MBR) systems treating municipal wastewater: a case studyen
dc.typeArticleen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalEnvironmental Science and Pollution Researchen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorHarb, Moustaphaen
kaust.authorHong, Pei-Yingen
kaust.grant.numberFCC/1/1971-06-01en
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