Metal Enhanced Fluorescence on Super-Hydrophobic Clusters of Gold Nanoparticles

Handle URI:
http://hdl.handle.net/10754/622043
Title:
Metal Enhanced Fluorescence on Super-Hydrophobic Clusters of Gold Nanoparticles
Authors:
Battista, Edmondo; Coluccio, Maria Laura; Alabastri, Alessandro; Barberio, Marianna; Causa, Filippo; Netti, Paolo Antonio; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 ) ; Gentile, Francesco
Abstract:
We used optical lithography, electroless deposition and deep reactive ion etching techniques to realize arrays of super-hydrophobic gold nanoparticles arranged in a hierarchical structure. At the micro-scale, silicon-micro pillars in the chip permit to manipulate and concentrate biological solutions, at the nano-scale, gold nanoparticles enable metal enhanced fluorescence (MEF) effects, whereby fluorescence signal of fluorophores in close proximity to a rough metal surface is amplified by orders of magnitude. Here, we demonstrated the device in the analysis of fluorescein derived gold-binding peptides (GBP-FITC). While super-hydrophobic schemes and MEF effects have been heretofore used in isolation, their integration in a platform may advance the current state of fluorescence-based sensing technology in medical diagnostics and biotechnology. This scheme may be employed in protein microarrays where the increased sensitivity of the device may enable the early detection of cancer biomarkers or other proteins of biomedical interest.
KAUST Department:
Physical Sciences and Engineering (PSE) Division
Citation:
Battista E, Coluccio ML, Alabastri A, Barberio M, Causa F, et al. (2016) Metal Enhanced Fluorescence on Super-Hydrophobic Clusters of Gold Nanoparticles. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2016.12.013.
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
15-Dec-2016
DOI:
10.1016/j.mee.2016.12.013
Type:
Article
ISSN:
0167-9317
Sponsors:
This work has been partially funded from the Italian Minister of Health (Project n. GR-2010-2320665) and by UE, MIUR and Ministero dello Sviluppo Economico (project ITEM (Infrastruttura per Tecnologie BioMEMS di Sensing Avanzato per Monitoraggio e Diagnostica Ambientale e Alimentare) PONa3_00077).
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0167931716305184
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBattista, Edmondoen
dc.contributor.authorColuccio, Maria Lauraen
dc.contributor.authorAlabastri, Alessandroen
dc.contributor.authorBarberio, Mariannaen
dc.contributor.authorCausa, Filippoen
dc.contributor.authorNetti, Paolo Antonioen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.contributor.authorGentile, Francescoen
dc.date.accessioned2016-12-20T05:52:11Z-
dc.date.available2016-12-20T05:52:11Z-
dc.date.issued2016-12-15en
dc.identifier.citationBattista E, Coluccio ML, Alabastri A, Barberio M, Causa F, et al. (2016) Metal Enhanced Fluorescence on Super-Hydrophobic Clusters of Gold Nanoparticles. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2016.12.013.en
dc.identifier.issn0167-9317en
dc.identifier.doi10.1016/j.mee.2016.12.013en
dc.identifier.urihttp://hdl.handle.net/10754/622043-
dc.description.abstractWe used optical lithography, electroless deposition and deep reactive ion etching techniques to realize arrays of super-hydrophobic gold nanoparticles arranged in a hierarchical structure. At the micro-scale, silicon-micro pillars in the chip permit to manipulate and concentrate biological solutions, at the nano-scale, gold nanoparticles enable metal enhanced fluorescence (MEF) effects, whereby fluorescence signal of fluorophores in close proximity to a rough metal surface is amplified by orders of magnitude. Here, we demonstrated the device in the analysis of fluorescein derived gold-binding peptides (GBP-FITC). While super-hydrophobic schemes and MEF effects have been heretofore used in isolation, their integration in a platform may advance the current state of fluorescence-based sensing technology in medical diagnostics and biotechnology. This scheme may be employed in protein microarrays where the increased sensitivity of the device may enable the early detection of cancer biomarkers or other proteins of biomedical interest.en
dc.description.sponsorshipThis work has been partially funded from the Italian Minister of Health (Project n. GR-2010-2320665) and by UE, MIUR and Ministero dello Sviluppo Economico (project ITEM (Infrastruttura per Tecnologie BioMEMS di Sensing Avanzato per Monitoraggio e Diagnostica Ambientale e Alimentare) PONa3_00077).en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0167931716305184en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microelectronic Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microelectronic Engineering, 15 December 2016. DOI: 10.1016/j.mee.2016.12.013en
dc.subjectMetal Enhanced Fluorescence (MEF)en
dc.subjectSuper-Hydrophobic Surfacesen
dc.subjectGold Nanoparticlesen
dc.subjectElectroless Depositionen
dc.subjectGold-binding Peptidesen
dc.subjectFluorescence Lifetime Microscopy (FLIM)en
dc.titleMetal Enhanced Fluorescence on Super-Hydrophobic Clusters of Gold Nanoparticlesen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalMicroelectronic Engineeringen
dc.eprint.versionPost-printen
dc.contributor.institutionInterdisciplinary Research Center on Biomaterials and DICMAPI, University Federico II, P.le Tecchio 80, Naples, Italyen
dc.contributor.institutionCenter for Advanced Biomaterials for HealthCare @CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, Naples, Italyen
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, University of Magna Graecia, 88100 Catanzaro, Italyen
dc.contributor.institutionPhysics and Astronomy Department, Rice University, Houston, Tx, USAen
dc.contributor.institutionIpat-lab, INRS-EMT, 1650 Boul Lionel Boulet, Varennes, (Qb), Canadaen
dc.contributor.institutionDepartment of Electrical Engineering and Information Technology, University Federico II, Naples, Italyen
kaust.authorDi Fabrizio, Enzo M.en
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