Laboratory injection molder for the fabrication of polymeric porous poly-epsilon-caprolactone scaffolds for preliminary mesenchymal stem cells tissue engineering applications

Handle URI:
http://hdl.handle.net/10754/622042
Title:
Laboratory injection molder for the fabrication of polymeric porous poly-epsilon-caprolactone scaffolds for preliminary mesenchymal stem cells tissue engineering applications
Authors:
Limongi, Tania; Lizzul, Lucia; Giugni, Andrea; Tirinato, Luca ( 0000-0001-9826-2129 ) ; Pagliari, Francesca ( 0000-0002-5547-222X ) ; Tan, Hua; Das, Gobind ( 0000-0003-0942-681X ) ; Moretti, Manola; Marini, Monica ( 0000-0001-8182-5239 ) ; Brusatin, Giovanna; Falqui, Andrea ( 0000-0002-1476-7742 ) ; Torre, Bruno; Di Benedetto, Cristiano; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
This study presents a simple and rapid fabrication technique involving injection molding and particle leaching (IM/PL) to fabricate the porous scaffold for tissue engineering applications. Sodium chloride (NaCl) and Sucrose are separately mixed with the poly-epsilon-caprolactone (PCL) granules using a screwed thermo regulated extruder, than the biocompatible scaffolds are fabricated through injection molding. The micro/nano structure of the samples and their different grade of porosity were characterized by scanning electron microscopy and mercury intrusion porosimetry. Bone marrow-derived mesenchymal stem cells are chose to cell culture and Hoechst 33342 staining was used to verify the biocompatibility of the polymeric porous surfaces. We concluded that, by using the same fast solvent free injection/leaching process, the use of Sucrose as porogen, instead of NaCl, allowed the obtainment of biocompatible scaffolds with a higher grade of porosity with suitable cell adhesion capacity for tissue engineering purpose.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division; Analytical Core Lab
Citation:
Limongi T, Lizzul L, Giugni A, Tirinato L, Pagliari F, et al. (2016) Laboratory injection molder for the fabrication of polymeric porous poly-epsilon-caprolactone scaffolds for preliminary mesenchymal stem cells tissue engineering applications. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2016.12.014.
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
16-Dec-2016
DOI:
10.1016/j.mee.2016.12.014
Type:
Article
ISSN:
0167-9317
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0167931716305202
Appears in Collections:
Articles; Analytical Core Lab; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLimongi, Taniaen
dc.contributor.authorLizzul, Luciaen
dc.contributor.authorGiugni, Andreaen
dc.contributor.authorTirinato, Lucaen
dc.contributor.authorPagliari, Francescaen
dc.contributor.authorTan, Huaen
dc.contributor.authorDas, Gobinden
dc.contributor.authorMoretti, Manolaen
dc.contributor.authorMarini, Monicaen
dc.contributor.authorBrusatin, Giovannaen
dc.contributor.authorFalqui, Andreaen
dc.contributor.authorTorre, Brunoen
dc.contributor.authorDi Benedetto, Cristianoen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2016-12-20T05:52:11Z-
dc.date.available2016-12-20T05:52:11Z-
dc.date.issued2016-12-16en
dc.identifier.citationLimongi T, Lizzul L, Giugni A, Tirinato L, Pagliari F, et al. (2016) Laboratory injection molder for the fabrication of polymeric porous poly-epsilon-caprolactone scaffolds for preliminary mesenchymal stem cells tissue engineering applications. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2016.12.014.en
dc.identifier.issn0167-9317en
dc.identifier.doi10.1016/j.mee.2016.12.014en
dc.identifier.urihttp://hdl.handle.net/10754/622042-
dc.description.abstractThis study presents a simple and rapid fabrication technique involving injection molding and particle leaching (IM/PL) to fabricate the porous scaffold for tissue engineering applications. Sodium chloride (NaCl) and Sucrose are separately mixed with the poly-epsilon-caprolactone (PCL) granules using a screwed thermo regulated extruder, than the biocompatible scaffolds are fabricated through injection molding. The micro/nano structure of the samples and their different grade of porosity were characterized by scanning electron microscopy and mercury intrusion porosimetry. Bone marrow-derived mesenchymal stem cells are chose to cell culture and Hoechst 33342 staining was used to verify the biocompatibility of the polymeric porous surfaces. We concluded that, by using the same fast solvent free injection/leaching process, the use of Sucrose as porogen, instead of NaCl, allowed the obtainment of biocompatible scaffolds with a higher grade of porosity with suitable cell adhesion capacity for tissue engineering purpose.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0167931716305202en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microelectronic Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microelectronic Engineering, 16 December 2016. DOI: 10.1016/j.mee.2016.12.014en
dc.subjectBiopolimersen
dc.subjectParticulate Leachingen
dc.subjectInjection Moldingen
dc.subjectTissue Engineeringen
dc.subjectStem Cellsen
dc.titleLaboratory injection molder for the fabrication of polymeric porous poly-epsilon-caprolactone scaffolds for preliminary mesenchymal stem cells tissue engineering applicationsen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentAnalytical Core Laben
dc.identifier.journalMicroelectronic Engineeringen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of industrial engineering, University of Padova, via Marzolo 9, 35131 Padova, Italyen
kaust.authorLimongi, Taniaen
kaust.authorLizzul, Luciaen
kaust.authorGiugni, Andreaen
kaust.authorTirinato, Lucaen
kaust.authorPagliari, Francescaen
kaust.authorTan, Huaen
kaust.authorDas, Gobinden
kaust.authorMarini, Monicaen
kaust.authorBrusatin, Giovannaen
kaust.authorFalqui, Andreaen
kaust.authorTorre, Brunoen
kaust.authorDi Benedetto, Cristianoen
kaust.authorDi Fabrizio, Enzo M.en
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