Transparent crosslinked ultrashort peptide hydrogel dressing with high shape-fidelity accelerates healing of full-thickness excision wounds

Handle URI:
http://hdl.handle.net/10754/622016
Title:
Transparent crosslinked ultrashort peptide hydrogel dressing with high shape-fidelity accelerates healing of full-thickness excision wounds
Authors:
Seow, Wei Yang; Salgado, Giorgiana; Lane, E. Birgitte; Hauser, Charlotte ( 0000-0001-8251-7246 )
Abstract:
Wound healing is a major burden of healthcare systems worldwide and hydrogel dressings offer a moist environment conducive to healing. We describe cysteine-containing ultrashort peptides that self-assemble spontaneously into hydrogels. After disulfide crosslinking, the optically-transparent hydrogels became significantly stiffer and exhibited high shape fidelity. The peptide sequence (LIVAGKC or LK6C) was then chosen for evaluation on mice with full-thickness excision wounds. Crosslinked LK6C hydrogels are handled easily with forceps during surgical procedures and offer an improvement over our earlier study of a non-crosslinked peptide hydrogel for burn wounds. LK6C showed low allergenic potential and failed to provoke any sensitivity when administered to guinea pigs in the Magnusson-Kligman maximization test. When applied topically as a dressing, the medium-infused LK6C hydrogel accelerated re-epithelialization compared to controls. The peptide hydrogel is thus safe for topical application and promotes a superior rate and quality of wound healing.
KAUST Department:
Laboratory for Nanomedicine, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
Citation:
Seow WY, Salgado G, Lane EB, Hauser CAE (2016) Transparent crosslinked ultrashort peptide hydrogel dressing with high shape-fidelity accelerates healing of full-thickness excision wounds. Scientific Reports 6: 32670. Available: http://dx.doi.org/10.1038/srep32670.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
7-Sep-2016
DOI:
10.1038/srep32670
Type:
Article
ISSN:
2045-2322
Sponsors:
We thank Declan Lunny (Institute of Medical Biology, Singapore) for valuable discussions and the blind scoring of tissues. This work was supported by the Institute of Bioengineering and Nanotechnology (Biomedical Research Council, Agency for Science, Technology and Research, Singapore).
Additional Links:
http://www.nature.com/articles/srep32670
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorSeow, Wei Yangen
dc.contributor.authorSalgado, Giorgianaen
dc.contributor.authorLane, E. Birgitteen
dc.contributor.authorHauser, Charlotteen
dc.date.accessioned2016-12-13T13:46:39Z-
dc.date.available2016-12-13T13:46:39Z-
dc.date.issued2016-09-07en
dc.identifier.citationSeow WY, Salgado G, Lane EB, Hauser CAE (2016) Transparent crosslinked ultrashort peptide hydrogel dressing with high shape-fidelity accelerates healing of full-thickness excision wounds. Scientific Reports 6: 32670. Available: http://dx.doi.org/10.1038/srep32670.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep32670en
dc.identifier.urihttp://hdl.handle.net/10754/622016-
dc.description.abstractWound healing is a major burden of healthcare systems worldwide and hydrogel dressings offer a moist environment conducive to healing. We describe cysteine-containing ultrashort peptides that self-assemble spontaneously into hydrogels. After disulfide crosslinking, the optically-transparent hydrogels became significantly stiffer and exhibited high shape fidelity. The peptide sequence (LIVAGKC or LK6C) was then chosen for evaluation on mice with full-thickness excision wounds. Crosslinked LK6C hydrogels are handled easily with forceps during surgical procedures and offer an improvement over our earlier study of a non-crosslinked peptide hydrogel for burn wounds. LK6C showed low allergenic potential and failed to provoke any sensitivity when administered to guinea pigs in the Magnusson-Kligman maximization test. When applied topically as a dressing, the medium-infused LK6C hydrogel accelerated re-epithelialization compared to controls. The peptide hydrogel is thus safe for topical application and promotes a superior rate and quality of wound healing.en
dc.description.sponsorshipWe thank Declan Lunny (Institute of Medical Biology, Singapore) for valuable discussions and the blind scoring of tissues. This work was supported by the Institute of Bioengineering and Nanotechnology (Biomedical Research Council, Agency for Science, Technology and Research, Singapore).en
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.nature.com/articles/srep32670en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleTransparent crosslinked ultrashort peptide hydrogel dressing with high shape-fidelity accelerates healing of full-thickness excision woundsen
dc.typeArticleen
dc.contributor.departmentLaboratory for Nanomedicine, King Abdullah University of Science and Technology, Thuwal, Saudi Arabiaen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionInstitute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore, Singaporeen
dc.contributor.institutionInstitute of Medical Biology, 8A Biomedical Grove, Singapore, Singaporeen
kaust.authorHauser, Charlotteen
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