Set anode potentials affect the electron fluxes and microbial community structure in propionate-fed microbial electrolysis cells

Handle URI:
http://hdl.handle.net/10754/622000
Title:
Set anode potentials affect the electron fluxes and microbial community structure in propionate-fed microbial electrolysis cells
Authors:
Rao, Hari Ananda ( 0000-0002-8916-1809 ) ; Katuri, Krishna; Logan, Bruce E.; Saikaly, Pascal ( 0000-0001-7678-3986 )
Abstract:
Anode potential has been shown to be a critical factor in the rate of acetate removal in microbial electrolysis cells (MECs), but studies with fermentable substrates and set potentials are lacking. Here, we examined the impact of three different set anode potentials (SAPs; −0.25, 0, and 0.25 V vs. standard hydrogen electrode) on the electrochemical performance, electron flux to various sinks, and anodic microbial community structure in two-chambered MECs fed with propionate. Electrical current (49–71%) and CH4 (22.9–41%) were the largest electron sinks regardless of the potentials tested. Among the three SAPs tested, 0 V showed the highest electron flux to electrical current (71 ± 5%) and the lowest flux to CH4 (22.9 ± 1.2%). In contrast, the SAP of −0.25 V had the lowest electron flux to current (49 ± 6%) and the highest flux to CH4 (41.1 ± 2%). The most dominant genera detected on the anode of all three SAPs based on 16S rRNA gene sequencing were Geobacter, Smithella and Syntrophobacter, but their relative abundance varied among the tested SAPs. Microbial community analysis implies that complete degradation of propionate in all the tested SAPs was facilitated by syntrophic interactions between fermenters and Geobacter at the anode and ferementers and hydrogenotrophic methanogens in suspension.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Hari AR, Katuri KP, Logan BE, Saikaly PE (2016) Set anode potentials affect the electron fluxes and microbial community structure in propionate-fed microbial electrolysis cells. Scientific Reports 6: 38690. Available: http://dx.doi.org/10.1038/srep38690.
Publisher:
Springer Nature
Journal:
Scientific Reports
KAUST Grant Number:
GRP-CF-2011-15-S; FCC/1/1971–05–01
Issue Date:
9-Dec-2016
DOI:
10.1038/srep38690
Type:
Article
ISSN:
2045-2322
Sponsors:
This work was sponsored by a Ph.D. fellowship, a Global Research Partnership-Collaborative Fellows Award (GRP-CF-2011-15-S) and Center Competitive Funding (FCC/1/1971–05–01) to P.E.S. from King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://www.nature.com/articles/srep38690
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorRao, Hari Anandaen
dc.contributor.authorKaturi, Krishnaen
dc.contributor.authorLogan, Bruce E.en
dc.contributor.authorSaikaly, Pascalen
dc.date.accessioned2016-12-12T08:35:48Z-
dc.date.available2016-12-12T08:35:48Z-
dc.date.issued2016-12-09en
dc.identifier.citationHari AR, Katuri KP, Logan BE, Saikaly PE (2016) Set anode potentials affect the electron fluxes and microbial community structure in propionate-fed microbial electrolysis cells. Scientific Reports 6: 38690. Available: http://dx.doi.org/10.1038/srep38690.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep38690en
dc.identifier.urihttp://hdl.handle.net/10754/622000-
dc.description.abstractAnode potential has been shown to be a critical factor in the rate of acetate removal in microbial electrolysis cells (MECs), but studies with fermentable substrates and set potentials are lacking. Here, we examined the impact of three different set anode potentials (SAPs; −0.25, 0, and 0.25 V vs. standard hydrogen electrode) on the electrochemical performance, electron flux to various sinks, and anodic microbial community structure in two-chambered MECs fed with propionate. Electrical current (49–71%) and CH4 (22.9–41%) were the largest electron sinks regardless of the potentials tested. Among the three SAPs tested, 0 V showed the highest electron flux to electrical current (71 ± 5%) and the lowest flux to CH4 (22.9 ± 1.2%). In contrast, the SAP of −0.25 V had the lowest electron flux to current (49 ± 6%) and the highest flux to CH4 (41.1 ± 2%). The most dominant genera detected on the anode of all three SAPs based on 16S rRNA gene sequencing were Geobacter, Smithella and Syntrophobacter, but their relative abundance varied among the tested SAPs. Microbial community analysis implies that complete degradation of propionate in all the tested SAPs was facilitated by syntrophic interactions between fermenters and Geobacter at the anode and ferementers and hydrogenotrophic methanogens in suspension.en
dc.description.sponsorshipThis work was sponsored by a Ph.D. fellowship, a Global Research Partnership-Collaborative Fellows Award (GRP-CF-2011-15-S) and Center Competitive Funding (FCC/1/1971–05–01) to P.E.S. from King Abdullah University of Science and Technology (KAUST).en
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.nature.com/articles/srep38690en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleSet anode potentials affect the electron fluxes and microbial community structure in propionate-fed microbial electrolysis cellsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionThe Pennsylvania State University, Department of Civil and Environmental Engineering, University Park, PA 16802, USAen
kaust.authorRao, Hari Anandaen
kaust.authorKaturi, Krishnaen
kaust.authorSaikaly, Pascalen
kaust.grant.numberGRP-CF-2011-15-Sen
kaust.grant.numberFCC/1/1971–05–01en
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