The efficacy of sewage influent-isolated bacteriophages on Pseudomonas aeruginosa in a mixed-species biofilm

Handle URI:
http://hdl.handle.net/10754/621991
Title:
The efficacy of sewage influent-isolated bacteriophages on Pseudomonas aeruginosa in a mixed-species biofilm
Authors:
Yap, Scott ( 0000-0002-8026-9194 )
Abstract:
The growth of environmentally persistent biofilms in cooling towers causes several associated problems, including microbiologically-induced corrosion (MIC) and biofouling. Current chemical control methods are not only ineffective against biofilms and costly to procure, they also have downstream environmental impacts when released untreated, or incur additional treatment costs. Bacteriophages are alternative biofilm control agents that have the potential to be more effective, cheaper to produce and yet have a more benign effect on the environment. In this study, biofilms grown under conditions simulating seawater fed cooling towers were characterized and the differences in growth and community make-up across time and different substrates were assessed. An MIC associated bacterium common in cooling tower water, P. aeruginosa, was chosen. Seven bacteriophage strains found to be effective against the chosen bacterium were isolated from wastewater influent. The relative effectiveness of these strains was measured against P. aeruginosa across different salinities. Separate biofilms fed with P. aeruginosa enriched seawater were characterized and the effectiveness of the isolated strains, singly and in cocktails, against the enriched biofilms was measured.
Advisors:
Hong, Pei-Ying ( 0000-0002-4474-6600 )
Committee Member:
Saikaly, Pascal ( 0000-0001-7678-3986 ) ; Pain, Arnab ( 0000-0002-1755-2819 )
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Program:
Environmental Science and Engineering
Issue Date:
Dec-2016
Type:
Thesis
Appears in Collections:
Theses

Full metadata record

DC FieldValue Language
dc.contributor.advisorHong, Pei-Yingen
dc.contributor.authorYap, Scotten
dc.date.accessioned2016-12-11T06:08:13Z-
dc.date.available2016-12-11T06:08:13Z-
dc.date.issued2016-12-
dc.identifier.urihttp://hdl.handle.net/10754/621991-
dc.description.abstractThe growth of environmentally persistent biofilms in cooling towers causes several associated problems, including microbiologically-induced corrosion (MIC) and biofouling. Current chemical control methods are not only ineffective against biofilms and costly to procure, they also have downstream environmental impacts when released untreated, or incur additional treatment costs. Bacteriophages are alternative biofilm control agents that have the potential to be more effective, cheaper to produce and yet have a more benign effect on the environment. In this study, biofilms grown under conditions simulating seawater fed cooling towers were characterized and the differences in growth and community make-up across time and different substrates were assessed. An MIC associated bacterium common in cooling tower water, P. aeruginosa, was chosen. Seven bacteriophage strains found to be effective against the chosen bacterium were isolated from wastewater influent. The relative effectiveness of these strains was measured against P. aeruginosa across different salinities. Separate biofilms fed with P. aeruginosa enriched seawater were characterized and the effectiveness of the isolated strains, singly and in cocktails, against the enriched biofilms was measured.en
dc.language.isoenen
dc.subjectBiocorrosionen
dc.subjectPhagesen
dc.subjectBiofilmen
dc.subjectSeawateren
dc.subjectPseudomonas aeruginosaen
dc.titleThe efficacy of sewage influent-isolated bacteriophages on Pseudomonas aeruginosa in a mixed-species biofilmen
dc.typeThesisen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberSaikaly, Pascalen
dc.contributor.committeememberPain, Arnaben
thesis.degree.disciplineEnvironmental Science and Engineeringen
thesis.degree.nameMaster of Scienceen
dc.person.id142814en
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