Correlation between membrane fluidity cellular development and stem cell differentiation

Handle URI:
http://hdl.handle.net/10754/621954
Title:
Correlation between membrane fluidity cellular development and stem cell differentiation
Authors:
Noutsi, Pakiza ( 0000-0001-5692-303X )
Abstract:
Cell membranes are made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During cellular development, such as neuronal differentiation, cell membranes undergo dramatic structural changes induced by proteins such as ARC and Cofilin among others in the case of synaptic modification. In this study we used the generalized polarization (GP) property of fluorescent probe Laurdan using two-photon microscopy to determine membrane fluidity as a function of time and for various cell lines. A low GP value corresponds to a higher fluidity and a higher GP value is associated with a more rigid membrane. Four different cell lines were monitored such as hN2, NIH3T3, HEK293 and L6 cells. As expected, NIH3T3 cells have more rigid membrane at earlier stages of their development. On the other hand neurons tend to have the highest membrane fluidity early in their development emphasizing its correlation with plasticity and the need for this malleability during differentiation. This study sheds light on the involvement of membrane fluidity during neuronal differentiation and development of other cell lines.
Advisors:
Chaieb, Sahraoui ( 0000-0002-8053-3610 )
Committee Member:
Magistretti, Pierre J. ( 0000-0002-6678-320X ) ; Khashab, Niveen M. ( 0000-0003-2728-0666 ) ; Gratton, Enrico
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Program:
Bioscience
Issue Date:
Dec-2016
Type:
Dissertation
Appears in Collections:
Dissertations

Full metadata record

DC FieldValue Language
dc.contributor.advisorChaieb, Sahraouien
dc.contributor.authorNoutsi, Pakizaen
dc.date.accessioned2016-12-06T13:30:47Z-
dc.date.available2016-12-06T13:30:47Z-
dc.date.issued2016-12-
dc.identifier.urihttp://hdl.handle.net/10754/621954-
dc.description.abstractCell membranes are made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During cellular development, such as neuronal differentiation, cell membranes undergo dramatic structural changes induced by proteins such as ARC and Cofilin among others in the case of synaptic modification. In this study we used the generalized polarization (GP) property of fluorescent probe Laurdan using two-photon microscopy to determine membrane fluidity as a function of time and for various cell lines. A low GP value corresponds to a higher fluidity and a higher GP value is associated with a more rigid membrane. Four different cell lines were monitored such as hN2, NIH3T3, HEK293 and L6 cells. As expected, NIH3T3 cells have more rigid membrane at earlier stages of their development. On the other hand neurons tend to have the highest membrane fluidity early in their development emphasizing its correlation with plasticity and the need for this malleability during differentiation. This study sheds light on the involvement of membrane fluidity during neuronal differentiation and development of other cell lines.en
dc.language.isoenen
dc.subjectNeuronen
dc.subjectdevelopmenten
dc.subjectstem cellen
dc.subjectDifferentiationen
dc.titleCorrelation between membrane fluidity cellular development and stem cell differentiationen
dc.typeDissertationen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberMagistretti, Pierre J.en
dc.contributor.committeememberKhashab, Niveen M.en
dc.contributor.committeememberGratton, Enricoen
thesis.degree.disciplineBioscienceen
thesis.degree.nameDoctor of Philosophyen
dc.person.id118516en
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