On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization

Handle URI:
http://hdl.handle.net/10754/621879
Title:
On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization
Authors:
Gooneratne, Chinthaka Pasan; Kodzius, Rimantas ( 0000-0001-9417-8894 ) ; Li, Fuquan ( 0000-0002-1573-597X ) ; Foulds, Ian G.; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
The remarkable advantages micro-chip platforms offer over cumbersome, time-consuming equipment currently in use for bio-analysis are well documented. In this research, a micro-chip that includes a unique magnetic actuator (MA) for the manipulation of superparamagnetic beads (SPBs), and a magnetoresistive sensor for the detection of SPBs is presented. A design methodology, which takes into account the magnetic volume of SPBs, diffusion and heat transfer phenomena, is presented with the aid of numerical analysis to optimize the parameters of the MA. The MA was employed as a magnetic flux generator and experimental analysis with commercially available COMPEL™ and Dynabeads® demonstrated the ability of the MA to precisely transport a small number of SPBs over long distances and concentrate SPBs to a sensing site for detection. Moreover, the velocities of COMPEL™ and Dynabead® SPBs were correlated to their magnetic volumes and were in good agreement with numerical model predictions. We found that 2.8 μm Dynabeads® travel faster, and can be attracted to a magnetic source from a longer distance, than 6.2 μm COMPEL™ beads at magnetic flux magnitudes of less than 10 mT. The micro-chip system could easily be integrated with electronic circuitry and microfluidic functions, paving the way for an on-chip biomolecule quantification device
KAUST Department:
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Citation:
Gooneratne C, Kodzius R, Li F, Foulds I, Kosel J (2016) On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization. Sensors 16: 1369. Available: http://dx.doi.org/10.3390/s16091369.
Publisher:
MDPI AG
Journal:
Sensors
Issue Date:
26-Aug-2016
DOI:
10.3390/s16091369
Type:
Article
ISSN:
1424-8220
Sponsors:
Research reported in this publication was supported by King Abdullah University of Science and Technology (KAUST). The authors acknowledge Filipe Cardoso of INESC Microsistemas & Nanotecnologias (INESC MN) for his help with the TMR sensor fabrication. The authors would like to thank Ren Jian, Zhihong Wang, Basil Chew and Xiang Yu, for their help with microfabrication and material characterization. The authors would also like to thank Professor Nicole Pamme, Professor Quentin Pankhurst, Professor Martin Gijs, Professor Menno Prins, Mark Tarn and Chengxun Liu for fruitful discussions on the research
Additional Links:
http://www.mdpi.com/1424-8220/16/9/1369
Appears in Collections:
Articles; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorGooneratne, Chinthaka Pasanen
dc.contributor.authorKodzius, Rimantasen
dc.contributor.authorLi, Fuquanen
dc.contributor.authorFoulds, Ian G.en
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2016-11-24T13:47:31Z-
dc.date.available2016-11-24T13:47:31Z-
dc.date.issued2016-08-26en
dc.identifier.citationGooneratne C, Kodzius R, Li F, Foulds I, Kosel J (2016) On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization. Sensors 16: 1369. Available: http://dx.doi.org/10.3390/s16091369.en
dc.identifier.issn1424-8220en
dc.identifier.doi10.3390/s16091369en
dc.identifier.urihttp://hdl.handle.net/10754/621879-
dc.description.abstractThe remarkable advantages micro-chip platforms offer over cumbersome, time-consuming equipment currently in use for bio-analysis are well documented. In this research, a micro-chip that includes a unique magnetic actuator (MA) for the manipulation of superparamagnetic beads (SPBs), and a magnetoresistive sensor for the detection of SPBs is presented. A design methodology, which takes into account the magnetic volume of SPBs, diffusion and heat transfer phenomena, is presented with the aid of numerical analysis to optimize the parameters of the MA. The MA was employed as a magnetic flux generator and experimental analysis with commercially available COMPEL™ and Dynabeads® demonstrated the ability of the MA to precisely transport a small number of SPBs over long distances and concentrate SPBs to a sensing site for detection. Moreover, the velocities of COMPEL™ and Dynabead® SPBs were correlated to their magnetic volumes and were in good agreement with numerical model predictions. We found that 2.8 μm Dynabeads® travel faster, and can be attracted to a magnetic source from a longer distance, than 6.2 μm COMPEL™ beads at magnetic flux magnitudes of less than 10 mT. The micro-chip system could easily be integrated with electronic circuitry and microfluidic functions, paving the way for an on-chip biomolecule quantification deviceen
dc.description.sponsorshipResearch reported in this publication was supported by King Abdullah University of Science and Technology (KAUST). The authors acknowledge Filipe Cardoso of INESC Microsistemas & Nanotecnologias (INESC MN) for his help with the TMR sensor fabrication. The authors would like to thank Ren Jian, Zhihong Wang, Basil Chew and Xiang Yu, for their help with microfabrication and material characterization. The authors would also like to thank Professor Nicole Pamme, Professor Quentin Pankhurst, Professor Martin Gijs, Professor Menno Prins, Mark Tarn and Chengxun Liu for fruitful discussions on the researchen
dc.publisherMDPI AGen
dc.relation.urlhttp://www.mdpi.com/1424-8220/16/9/1369en
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectmagnetoresistive sensoren
dc.subjectsuperparamagnetic beadsen
dc.subjectmicro-chipen
dc.subjectmicro-actuatoren
dc.subjectnanoparticlesen
dc.subjectmagnetophoresisen
dc.subjectmagnetic detectionen
dc.subjectnumerical analysisen
dc.subjectlab-on-a-chipen
dc.subjectmicrofabricationen
dc.titleOn-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterizationen
dc.typeArticleen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalSensorsen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorGooneratne, Chinthaka Pasanen
kaust.authorKodzius, Rimantasen
kaust.authorLi, Fuquanen
kaust.authorFoulds, Ian G.en
kaust.authorKosel, Jürgenen
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