Nitrogen assimilation system in maize is regulated by developmental and tissue-specific mechanisms

Handle URI:
http://hdl.handle.net/10754/621720
Title:
Nitrogen assimilation system in maize is regulated by developmental and tissue-specific mechanisms
Authors:
Plett, Darren; Holtham, Luke; Baumann, Ute; Kalashyan, Elena; Francis, Karen; Enju, Akiko; Toubia, John; Roessner, Ute; Bacic, Antony; Rafalski, Antoni; Dhugga, Kanwarpal S.; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Garnett, Trevor; Kaiser, Brent N.
Abstract:
Key message: We found metabolites, enzyme activities and enzyme transcript abundances vary significantly across the maize lifecycle, but weak correlation exists between the three groups. We identified putative genes regulating nitrate assimilation. Abstract: Progress in improving nitrogen (N) use efficiency (NUE) of crop plants has been hampered by the complexity of the N uptake and utilisation systems. To understand this complexity we measured the activities of seven enzymes and ten metabolites related to N metabolism in the leaf and root tissues of Gaspe Flint maize plants grown in 0.5 or 2.5 mM NO3 − throughout the lifecycle. The amino acids had remarkably similar profiles across the lifecycle except for transient responses, which only appeared in the leaves for aspartate or in the roots for asparagine, serine and glycine. The activities of the enzymes for N assimilation were also coordinated to a certain degree, most noticeably with a peak in root activity late in the lifecycle, but with wide variation in the activity levels over the course of development. We analysed the transcriptional data for gene sets encoding the measured enzymes and found that, unlike the enzyme activities, transcript levels of the corresponding genes did not exhibit the same coordination across the lifecycle and were only weakly correlated with the levels of various amino acids or individual enzyme activities. We identified gene sets which were correlated with the enzyme activity profiles, including seven genes located within previously known quantitative trait loci for enzyme activities and hypothesise that these genes are important for the regulation of enzyme activities. This work provides insights into the complexity of the N assimilation system throughout development and identifies candidate regulatory genes, which warrant further investigation in efforts to improve NUE in crop plants. © 2016, Springer Science+Business Media Dordrecht.
KAUST Department:
Center for Desert Agriculture
Citation:
Plett D, Holtham L, Baumann U, Kalashyan E, Francis K, et al. (2016) Nitrogen assimilation system in maize is regulated by developmental and tissue-specific mechanisms. Plant Molecular Biology 92: 293–312. Available: http://dx.doi.org/10.1007/s11103-016-0512-5.
Publisher:
Springer Nature
Journal:
Plant Molecular Biology
Issue Date:
10-Aug-2016
DOI:
10.1007/s11103-016-0512-5
Type:
Article
ISSN:
0167-4412; 1573-5028
Sponsors:
Australian Research Council[LP130101055]
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorPlett, Darrenen
dc.contributor.authorHoltham, Lukeen
dc.contributor.authorBaumann, Uteen
dc.contributor.authorKalashyan, Elenaen
dc.contributor.authorFrancis, Karenen
dc.contributor.authorEnju, Akikoen
dc.contributor.authorToubia, Johnen
dc.contributor.authorRoessner, Uteen
dc.contributor.authorBacic, Antonyen
dc.contributor.authorRafalski, Antonien
dc.contributor.authorDhugga, Kanwarpal S.en
dc.contributor.authorTester, Mark A.en
dc.contributor.authorGarnett, Trevoren
dc.contributor.authorKaiser, Brent N.en
dc.date.accessioned2016-11-03T13:23:30Z-
dc.date.available2016-11-03T13:23:30Z-
dc.date.issued2016-08-10en
dc.identifier.citationPlett D, Holtham L, Baumann U, Kalashyan E, Francis K, et al. (2016) Nitrogen assimilation system in maize is regulated by developmental and tissue-specific mechanisms. Plant Molecular Biology 92: 293–312. Available: http://dx.doi.org/10.1007/s11103-016-0512-5.en
dc.identifier.issn0167-4412en
dc.identifier.issn1573-5028en
dc.identifier.doi10.1007/s11103-016-0512-5en
dc.identifier.urihttp://hdl.handle.net/10754/621720-
dc.description.abstractKey message: We found metabolites, enzyme activities and enzyme transcript abundances vary significantly across the maize lifecycle, but weak correlation exists between the three groups. We identified putative genes regulating nitrate assimilation. Abstract: Progress in improving nitrogen (N) use efficiency (NUE) of crop plants has been hampered by the complexity of the N uptake and utilisation systems. To understand this complexity we measured the activities of seven enzymes and ten metabolites related to N metabolism in the leaf and root tissues of Gaspe Flint maize plants grown in 0.5 or 2.5 mM NO3 − throughout the lifecycle. The amino acids had remarkably similar profiles across the lifecycle except for transient responses, which only appeared in the leaves for aspartate or in the roots for asparagine, serine and glycine. The activities of the enzymes for N assimilation were also coordinated to a certain degree, most noticeably with a peak in root activity late in the lifecycle, but with wide variation in the activity levels over the course of development. We analysed the transcriptional data for gene sets encoding the measured enzymes and found that, unlike the enzyme activities, transcript levels of the corresponding genes did not exhibit the same coordination across the lifecycle and were only weakly correlated with the levels of various amino acids or individual enzyme activities. We identified gene sets which were correlated with the enzyme activity profiles, including seven genes located within previously known quantitative trait loci for enzyme activities and hypothesise that these genes are important for the regulation of enzyme activities. This work provides insights into the complexity of the N assimilation system throughout development and identifies candidate regulatory genes, which warrant further investigation in efforts to improve NUE in crop plants. © 2016, Springer Science+Business Media Dordrecht.en
dc.description.sponsorshipAustralian Research Council[LP130101055]en
dc.publisherSpringer Natureen
dc.subjectAmino acidsen
dc.titleNitrogen assimilation system in maize is regulated by developmental and tissue-specific mechanismsen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalPlant Molecular Biologyen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics, Waite Research Institute, University of Adelaide, Adelaide, SA, Australiaen
dc.contributor.institutionSchool of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide, Adelaide, SA, Australiaen
dc.contributor.institutionACRF South Australian Cancer Genomics Facility, Centre for Cancer Biology, SA Pathology, Adelaide, SA, Australiaen
dc.contributor.institutionSchool of Molecular and Biomedical Science, The University of Adelaide, Adelaide, SA, Australiaen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics, School of BioSciences, The University of Melbourne, Parkville, VIC, Australiaen
dc.contributor.institutionMetabolomics Australia, School of BioSciences, The University of Melbourne, Parkville, VIC, Australiaen
dc.contributor.institutionARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Parkville, VIC, Australiaen
dc.contributor.institutionDuPont Pioneer, Wilmington, DE, United Statesen
dc.contributor.institutionDuPont Pioneer, Johnston, IA, United Statesen
dc.contributor.institutionInternational Maize and Wheat Improvement Center (CIMMYT), Carretera México Veracruz, Km. 45, El Batán, Texcoco, Estado De México, United Statesen
dc.contributor.institutionThe Plant Accelerator, Australian Plant Phenomics Facility, The University of Adelaide, PMB 1, Glen Osmond, Australiaen
dc.contributor.institutionCentre For Carbon Water and Food, The Faculty of Agriculture and Environment, The University of Sydney, Camden, NSW, Australiaen
kaust.authorTester, Mark A.en
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