Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors

Handle URI:
http://hdl.handle.net/10754/621623
Title:
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors
Authors:
Dineshram, Ramadoss; Chandramouli, Kondethimmanahalli ( 0000-0002-0104-1993 ) ; Ko, Ginger Wai Kuen; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Qian, Pei-Yuan; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Thiyagarajan, Vengatesen
Abstract:
The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs. © 2016 John Wiley & Sons Ltd.
KAUST Department:
Applied Mathematics and Computational Science Program; KAUST Environmental Epigenetics Research Program (KEEP)
Citation:
Dineshram R, Chandramouli K, Ko GWK, Zhang H, Qian P-Y, et al. (2016) Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology 22: 2054–2068. Available: http://dx.doi.org/10.1111/gcb.13249.
Publisher:
Wiley-Blackwell
Journal:
Global Change Biology
Issue Date:
19-Mar-2016
DOI:
10.1111/gcb.13249
Type:
Article
ISSN:
1354-1013
Sponsors:
The authors would like to thank Ziniu Yu and Yang Zhang of South China Sea Institute of Oceanology for providing the oysters used in our experiments and for helping us with RT-PCR analysis. We thank Ivan Chiu (University of Hong Kong), and Yun Lam (City University of Hong Kong) for technical support and for providing access to sample preparation and data analysis. The collaboration between VT and PYQ is supported by the State Key Laboratory in Marine Pollution of City University of Hong Kong. We thank Gabriela Bindea of Cordeliers Research Center for adding Crassostrea gigas annotation resources in ClueGO for pathway analysis. We thank P. Sriyutha Murthy (BARC, Kalpakkam, India) for proofreading. We are also grateful to the three anonymous reviewers and the editor for their substantial contributions to this article. This study was funded by three GRF grants from the HKSAR-RGC (Grant Numbers: 705112P, 17304914, and 705511P).
Is Supplemented By:
Dineshram, R., Chandramouli, K., Ko, W. K. G., Zhang, H., Qian, P. Y., Ravasi, T., & Thiyagarajan, V. (2016). Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068 [Data set]. PANGAEA - Data Publisher for Earth & Environmental Science. https://doi.org/10.1594/pangaea.867318; DOI:10.1594/PANGAEA.867318; HANDLE:http://hdl.handle.net/10754/624161
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/gcb.13249/full
Appears in Collections:
Articles; Applied Mathematics and Computational Science Program

Full metadata record

DC FieldValue Language
dc.contributor.authorDineshram, Ramadossen
dc.contributor.authorChandramouli, Kondethimmanahallien
dc.contributor.authorKo, Ginger Wai Kuenen
dc.contributor.authorZhang, Huomingen
dc.contributor.authorQian, Pei-Yuanen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorThiyagarajan, Vengatesenen
dc.date.accessioned2016-11-03T13:21:12Z-
dc.date.available2016-11-03T13:21:12Z-
dc.date.issued2016-03-19en
dc.identifier.citationDineshram R, Chandramouli K, Ko GWK, Zhang H, Qian P-Y, et al. (2016) Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology 22: 2054–2068. Available: http://dx.doi.org/10.1111/gcb.13249.en
dc.identifier.issn1354-1013en
dc.identifier.doi10.1111/gcb.13249en
dc.identifier.urihttp://hdl.handle.net/10754/621623-
dc.description.abstractThe metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs. © 2016 John Wiley & Sons Ltd.en
dc.description.sponsorshipThe authors would like to thank Ziniu Yu and Yang Zhang of South China Sea Institute of Oceanology for providing the oysters used in our experiments and for helping us with RT-PCR analysis. We thank Ivan Chiu (University of Hong Kong), and Yun Lam (City University of Hong Kong) for technical support and for providing access to sample preparation and data analysis. The collaboration between VT and PYQ is supported by the State Key Laboratory in Marine Pollution of City University of Hong Kong. We thank Gabriela Bindea of Cordeliers Research Center for adding Crassostrea gigas annotation resources in ClueGO for pathway analysis. We thank P. Sriyutha Murthy (BARC, Kalpakkam, India) for proofreading. We are also grateful to the three anonymous reviewers and the editor for their substantial contributions to this article. This study was funded by three GRF grants from the HKSAR-RGC (Grant Numbers: 705112P, 17304914, and 705511P).en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/gcb.13249/fullen
dc.subjectClimate changeen
dc.subjectCrassostrea gigasen
dc.subjectEnvironmental proteomicsen
dc.subjectiTRAQen
dc.subjectMultiple stressorsen
dc.subjectOcean acidificationen
dc.titleQuantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressorsen
dc.typeArticleen
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.contributor.departmentKAUST Environmental Epigenetics Research Program (KEEP)en
dc.identifier.journalGlobal Change Biologyen
dc.contributor.institutionThe Swire Institute of Marine Science and School of Biological Sciences; The University of Hong Kong; Pok Fu Lam Road, Hong Kong Island; Hong Kong SAR Chinaen
dc.contributor.institutionDivision of Life Science; Hong Kong University of Science and Technology; Clear Water Bay, Kowloon; Hong Kong SAR Chinaen
kaust.authorChandramouli, Kondethimmanahallien
kaust.authorZhang, Huomingen
kaust.authorRavasi, Timothyen
dc.relation.isSupplementedByDineshram, R., Chandramouli, K., Ko, W. K. G., Zhang, H., Qian, P. Y., Ravasi, T., & Thiyagarajan, V. (2016). Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068 [Data set]. PANGAEA - Data Publisher for Earth & Environmental Science. https://doi.org/10.1594/pangaea.867318en
dc.relation.isSupplementedByDOI:10.1594/PANGAEA.867318en
dc.relation.isSupplementedByHANDLE:http://hdl.handle.net/10754/624161en
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