Targeted genome regulation via synthetic programmable transcriptional regulators

Handle URI:
http://hdl.handle.net/10754/621471
Title:
Targeted genome regulation via synthetic programmable transcriptional regulators
Authors:
Piatek, Agnieszka Anna ( 0000-0001-9587-6360 ) ; Mahfouz, Magdy M. ( 0000-0002-0616-6365 )
Abstract:
Regulation of gene transcription controls cellular functions and coordinates responses to developmental, physiological and environmental cues. Precise and efficient molecular tools are needed to characterize the functions of single and multiple genes in linear and interacting pathways in a native context. Modular DNA-binding domains from zinc fingers (ZFs) and transcriptional activator-like proteins (TALE) are amenable to bioengineering to bind DNA target sequences of interest. As a result, ZF and TALE proteins were used to develop synthetic programmable transcription factors. However, these systems are limited by the requirement to re-engineer proteins for each new target sequence. The clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR associated 9 (Cas9) genome editing tool was recently repurposed for targeted transcriptional regulation by inactivation of the nuclease activity of Cas9. Due to the facile engineering, simplicity, precision and amenability to library construction, the CRISPR/Cas9 system is poised to revolutionize the functional genomics field across diverse eukaryotic species. In this review, we discuss the development of synthetic customizable transcriptional regulators and provide insights into their current and potential applications, with special emphasis on plant systems, in characterization of gene functions, elucidation of molecular mechanisms and their biotechnological applications. © 2016 Informa UK Limited, trading as Taylor & Francis Group
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Desert Agriculture Initiative
Citation:
Piatek A, Mahfouz MM (2016) Targeted genome regulation via synthetic programmable transcriptional regulators. Critical Reviews in Biotechnology: 1–12. Available: http://dx.doi.org/10.3109/07388551.2016.1165180.
Publisher:
Informa Healthcare
Journal:
Critical Reviews in Biotechnology
Issue Date:
19-Apr-2016
DOI:
10.3109/07388551.2016.1165180
Type:
Article
ISSN:
0738-8551; 1549-7801
Sponsors:
King Abdullah University of Science and Technology[CRG4]
Appears in Collections:
Articles; Bioscience Program; Plant Science Program; Desert Agriculture Initiative; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPiatek, Agnieszka Annaen
dc.contributor.authorMahfouz, Magdy M.en
dc.date.accessioned2016-11-03T08:30:09Z-
dc.date.available2016-11-03T08:30:09Z-
dc.date.issued2016-04-19en
dc.identifier.citationPiatek A, Mahfouz MM (2016) Targeted genome regulation via synthetic programmable transcriptional regulators. Critical Reviews in Biotechnology: 1–12. Available: http://dx.doi.org/10.3109/07388551.2016.1165180.en
dc.identifier.issn0738-8551en
dc.identifier.issn1549-7801en
dc.identifier.doi10.3109/07388551.2016.1165180en
dc.identifier.urihttp://hdl.handle.net/10754/621471-
dc.description.abstractRegulation of gene transcription controls cellular functions and coordinates responses to developmental, physiological and environmental cues. Precise and efficient molecular tools are needed to characterize the functions of single and multiple genes in linear and interacting pathways in a native context. Modular DNA-binding domains from zinc fingers (ZFs) and transcriptional activator-like proteins (TALE) are amenable to bioengineering to bind DNA target sequences of interest. As a result, ZF and TALE proteins were used to develop synthetic programmable transcription factors. However, these systems are limited by the requirement to re-engineer proteins for each new target sequence. The clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR associated 9 (Cas9) genome editing tool was recently repurposed for targeted transcriptional regulation by inactivation of the nuclease activity of Cas9. Due to the facile engineering, simplicity, precision and amenability to library construction, the CRISPR/Cas9 system is poised to revolutionize the functional genomics field across diverse eukaryotic species. In this review, we discuss the development of synthetic customizable transcriptional regulators and provide insights into their current and potential applications, with special emphasis on plant systems, in characterization of gene functions, elucidation of molecular mechanisms and their biotechnological applications. © 2016 Informa UK Limited, trading as Taylor & Francis Groupen
dc.description.sponsorshipKing Abdullah University of Science and Technology[CRG4]en
dc.publisherInforma Healthcareen
dc.subjectCRISPR/Cas9 systemen
dc.subjectfunctional genomicsen
dc.subjectsynthetic site-specific nucleasesen
dc.subjectsynthetic transcriptional regulatorsen
dc.subjecttargeted genome regulationen
dc.subjecttranscriptional activator-like effectorsen
dc.subjectzinc fingersen
dc.titleTargeted genome regulation via synthetic programmable transcriptional regulatorsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentPlant Science Programen
dc.contributor.departmentDesert Agriculture Initiativeen
dc.identifier.journalCritical Reviews in Biotechnologyen
kaust.authorPiatek, Agnieszka Annaen
kaust.authorMahfouz, Magdy M.en
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