Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

Handle URI:
http://hdl.handle.net/10754/621445
Title:
Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers
Authors:
Perozziello, Gerardo; Candeloro, Patrizio; De Grazia, Antonio ( 0000-0002-8935-7801 ) ; Esposito, Francesco; Allione, Marco ( 0000-0003-0757-9791 ) ; Coluccio, Maria Laura; Tallerico, Rossana; Valpapuram, Immanuel; Tirinato, Luca ( 0000-0001-9826-2129 ) ; Das, Gobind ( 0000-0003-0942-681X ) ; Giugni, Andrea; Torre, Bruno; Veltri, Pierangelo; Kruhne, Ulrich; Della Valle, Giuseppe; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562). © 2015 Optical Society of America.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Physical Sciences and Engineering (PSE) Division
Citation:
Perozziello G, Candeloro P, De Grazia A, Esposito F, Allione M, et al. (2015) Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers. Optics Express 24: A180. Available: http://dx.doi.org/10.1364/oe.24.00a180.
Publisher:
The Optical Society
Journal:
Optics Express
Issue Date:
11-Dec-2015
DOI:
10.1364/oe.24.00a180
Type:
Article
ISSN:
1094-4087
Sponsors:
This work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338), the project for Young researchers financed from the Ministry of Health "High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices" (CUP J65C13001350001, project no. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University "Magna Graecia" of Catanzaro.
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPerozziello, Gerardoen
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorDe Grazia, Antonioen
dc.contributor.authorEsposito, Francescoen
dc.contributor.authorAllione, Marcoen
dc.contributor.authorColuccio, Maria Lauraen
dc.contributor.authorTallerico, Rossanaen
dc.contributor.authorValpapuram, Immanuelen
dc.contributor.authorTirinato, Lucaen
dc.contributor.authorDas, Gobinden
dc.contributor.authorGiugni, Andreaen
dc.contributor.authorTorre, Brunoen
dc.contributor.authorVeltri, Pierangeloen
dc.contributor.authorKruhne, Ulrichen
dc.contributor.authorDella Valle, Giuseppeen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2016-11-03T08:29:29Z-
dc.date.available2016-11-03T08:29:29Z-
dc.date.issued2015-12-11en
dc.identifier.citationPerozziello G, Candeloro P, De Grazia A, Esposito F, Allione M, et al. (2015) Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers. Optics Express 24: A180. Available: http://dx.doi.org/10.1364/oe.24.00a180.en
dc.identifier.issn1094-4087en
dc.identifier.doi10.1364/oe.24.00a180en
dc.identifier.urihttp://hdl.handle.net/10754/621445-
dc.description.abstractIn this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562). © 2015 Optical Society of America.en
dc.description.sponsorshipThis work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338), the project for Young researchers financed from the Ministry of Health "High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices" (CUP J65C13001350001, project no. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University "Magna Graecia" of Catanzaro.en
dc.publisherThe Optical Societyen
dc.titleMicrofluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimersen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalOptics Expressen
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, BioNEM lab, University Magna Graecia of Catanzaro, Catanzaro, Italyen
dc.contributor.institutionDepartment of Surgical and Medical Science, University Magna Graecia of Catanzaro, Catanzaro, Italyen
dc.contributor.institutionDepartment of Chemical and Biochemical Engineering, Technical University of Denmark (DTU), Lyngby, Denmarken
dc.contributor.institutionDepartment of Physics, Istituto di Fotonica E Nanotecnologie - CNR, Politecnico di Milano, Milano, Italyen
kaust.authorDe Grazia, Antonioen
kaust.authorAllione, Marcoen
kaust.authorColuccio, Maria Lauraen
kaust.authorValpapuram, Immanuelen
kaust.authorTirinato, Lucaen
kaust.authorDas, Gobinden
kaust.authorGiugni, Andreaen
kaust.authorTorre, Brunoen
kaust.authorDi Fabrizio, Enzo M.en
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