Physico-chemical properties and biological effects of diesel and biomass particles

Handle URI:
http://hdl.handle.net/10754/621439
Title:
Physico-chemical properties and biological effects of diesel and biomass particles
Authors:
Longhin, Eleonora; Gualtieri, Maurizio; Capasso, Laura; Bengalli, Rossella; Mollerup, Steen; Holme, Jørn A.; Øvrevik, Johan; Casadei, Simone; Di Benedetto, Cristiano; Parenti, Paolo; Camatini, Marina
Abstract:
© 2016 Elsevier Ltd. Diesel combustion and solid biomass burning are the major sources of ultrafine particles (UFP) in urbanized areas. Cardiovascular and pulmonary diseases, including lung cancer, are possible outcomes of combustion particles exposure, but differences in particles properties seem to influence their biological effects.Here the physico-chemical properties and biological effects of diesel and biomass particles, produced under controlled laboratory conditions, have been characterized. Diesel UFP were sampled from a Euro 4 light duty vehicle without DPF fuelled by commercial diesel and run over a chassis dyno. Biomass UFP were collected from a modern automatic 25 kW boiler propelled by prime quality spruce pellet. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) images of both diesel and biomass samples showed aggregates of soot particles, but in biomass samples ash particles were also present. Chemical characterization showed that metals and PAHs total content was higher in diesel samples compared to biomass ones.Human bronchial epithelial (HBEC3) cells were exposed to particles for up to 2 weeks. Changes in the expression of genes involved in xenobiotic metabolism were observed after exposure to both UFP already after 24 h. However, only diesel particles modulated the expression of genes involved in inflammation, oxidative stress and epithelial-to-mesenchymal transition (EMT), increased the release of inflammatory mediators and caused phenotypical alterations, mostly after two weeks of exposure.These results show that diesel UFP affected cellular processes involved in lung and cardiovascular diseases and cancer. Biomass particles exerted low biological activity compared to diesel UFP. This evidence emphasizes that the study of different emission sources contribution to ambient PM toxicity may have a fundamental role in the development of more effective strategies for air quality improvement.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Longhin E, Gualtieri M, Capasso L, Bengalli R, Mollerup S, et al. (2016) Physico-chemical properties and biological effects of diesel and biomass particles. Environmental Pollution 215: 366–375. Available: http://dx.doi.org/10.1016/j.envpol.2016.05.015.
Publisher:
Elsevier BV
Journal:
Environmental Pollution
Issue Date:
15-May-2016
DOI:
10.1016/j.envpol.2016.05.015
Type:
Article
ISSN:
0269-7491
Sponsors:
Norges Forskningsråd[228143]; Fondazione Cariplo[2013-1038]
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLonghin, Eleonoraen
dc.contributor.authorGualtieri, Maurizioen
dc.contributor.authorCapasso, Lauraen
dc.contributor.authorBengalli, Rossellaen
dc.contributor.authorMollerup, Steenen
dc.contributor.authorHolme, Jørn A.en
dc.contributor.authorØvrevik, Johanen
dc.contributor.authorCasadei, Simoneen
dc.contributor.authorDi Benedetto, Cristianoen
dc.contributor.authorParenti, Paoloen
dc.contributor.authorCamatini, Marinaen
dc.date.accessioned2016-11-03T08:29:21Z-
dc.date.available2016-11-03T08:29:21Z-
dc.date.issued2016-05-15en
dc.identifier.citationLonghin E, Gualtieri M, Capasso L, Bengalli R, Mollerup S, et al. (2016) Physico-chemical properties and biological effects of diesel and biomass particles. Environmental Pollution 215: 366–375. Available: http://dx.doi.org/10.1016/j.envpol.2016.05.015.en
dc.identifier.issn0269-7491en
dc.identifier.doi10.1016/j.envpol.2016.05.015en
dc.identifier.urihttp://hdl.handle.net/10754/621439-
dc.description.abstract© 2016 Elsevier Ltd. Diesel combustion and solid biomass burning are the major sources of ultrafine particles (UFP) in urbanized areas. Cardiovascular and pulmonary diseases, including lung cancer, are possible outcomes of combustion particles exposure, but differences in particles properties seem to influence their biological effects.Here the physico-chemical properties and biological effects of diesel and biomass particles, produced under controlled laboratory conditions, have been characterized. Diesel UFP were sampled from a Euro 4 light duty vehicle without DPF fuelled by commercial diesel and run over a chassis dyno. Biomass UFP were collected from a modern automatic 25 kW boiler propelled by prime quality spruce pellet. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) images of both diesel and biomass samples showed aggregates of soot particles, but in biomass samples ash particles were also present. Chemical characterization showed that metals and PAHs total content was higher in diesel samples compared to biomass ones.Human bronchial epithelial (HBEC3) cells were exposed to particles for up to 2 weeks. Changes in the expression of genes involved in xenobiotic metabolism were observed after exposure to both UFP already after 24 h. However, only diesel particles modulated the expression of genes involved in inflammation, oxidative stress and epithelial-to-mesenchymal transition (EMT), increased the release of inflammatory mediators and caused phenotypical alterations, mostly after two weeks of exposure.These results show that diesel UFP affected cellular processes involved in lung and cardiovascular diseases and cancer. Biomass particles exerted low biological activity compared to diesel UFP. This evidence emphasizes that the study of different emission sources contribution to ambient PM toxicity may have a fundamental role in the development of more effective strategies for air quality improvement.en
dc.description.sponsorshipNorges Forskningsråd[228143]en
dc.description.sponsorshipFondazione Cariplo[2013-1038]en
dc.publisherElsevier BVen
dc.subjectBiomassen
dc.subjectChemical characterizationen
dc.subjectDieselen
dc.subjectIn vitro toxicologyen
dc.subjectUltrafine particlesen
dc.titlePhysico-chemical properties and biological effects of diesel and biomass particlesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalEnvironmental Pollutionen
dc.contributor.institutionPolaris Research Centre, Dept. of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza, 1, 20126, Milan, Italyen
dc.contributor.institutionItalian National Agency for New Technologies, Energy and Sustainable Economic Development - ENEA-SSPT-MET-INAT, Strada per Crescentino 41, 13040, Saluggia, Vercelli, Italyen
dc.contributor.institutionDept. of Biological and Chemical Working Environment, National Institute of Occupational Health, N-0033, Oslo, Norwayen
dc.contributor.institutionDomain for Infection Control and Environmental Health, Norwegian Institute of Public Health, N-0403 Oslo, Norwayen
dc.contributor.institutionInnovhub-SSI Fuels Division, Via Galileo Galilei, 1, 20097, San Donato Milanese, Milan, Italyen
kaust.authorDi Benedetto, Cristianoen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.