Comparative and quantitative proteomics reveal the adaptive strategies of oyster larvae to ocean acidification

Handle URI:
http://hdl.handle.net/10754/621385
Title:
Comparative and quantitative proteomics reveal the adaptive strategies of oyster larvae to ocean acidification
Authors:
Dineshram, R.; Q., Quan; Sharma, Rakesh; Chandramouli, Kondethimmanahalli ( 0000-0002-0104-1993 ) ; Yalamanchili, Hari Krishna; Chu, Ivan; Thiyagarajan, Vengatesen
Abstract:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Decreasing pH due to anthropogenic CO2 inputs, called ocean acidification (OA), can make coastal environments unfavorable for oysters. This is a serious socioeconomical issue for China which supplies >70% of the world's edible oysters. Here, we present an iTRAQ-based protein profiling approach for the detection and quantification of proteome changes under OA in the early life stage of a commercially important oyster, Crassostrea hongkongensis. Availability of complete genome sequence for the pacific oyster (Crassostrea gigas) enabled us to confidently quantify over 1500 proteins in larval oysters. Over 7% of the proteome was altered in response to OA at pHNBS 7.6. Analysis of differentially expressed proteins and their associated functional pathways showed an upregulation of proteins involved in calcification, metabolic processes, and oxidative stress, each of which may be important in physiological adaptation of this species to OA. The downregulation of cytoskeletal and signal transduction proteins, on the other hand, might have impaired cellular dynamics and organelle development under OA. However, there were no significant detrimental effects in developmental processes such as metamorphic success. Implications of the differentially expressed proteins and metabolic pathways in the development of OA resistance in oyster larvae are discussed. The MS proteomics data have been deposited to the ProteomeXchange with identifiers PXD002138 (http://proteomecentral.proteomexchange.org/dataset/PXD002138).
KAUST Department:
Biological; Environmental Sciences & Engineering; King Abdullah University of Science and Technology; Thuwal Kingdom of Saudi Arabia
Citation:
Dineshram R, Q. Q, Sharma R, Chandramouli K, Yalamanchili HK, et al. (2015) Comparative and quantitative proteomics reveal the adaptive strategies of oyster larvae to ocean acidification. PROTEOMICS 15: 4120–4134. Available: http://dx.doi.org/10.1002/pmic.201500198.
Publisher:
Wiley-Blackwell
Journal:
PROTEOMICS
Issue Date:
28-Oct-2015
DOI:
10.1002/pmic.201500198
Type:
Article
ISSN:
1615-9853
Sponsors:
We are grateful to two anonymous reviewers and the editor who offered several constructive comments and criticisms on this article. The authors would like to thank Ziniu Yu and Shu Xiao of South China Sea Institute for Oceanology for providing the oysters and larval culture facilities used in our experiments. We thank Yun Lam (City University of Hong Kong) for providing various constructive comments during the proteomics data analysis and interpretation. This study was funded by three GRF grants from the HKSAR-RGC (grant numbers: 705511P, 705112P, and 17304914).
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Full metadata record

DC FieldValue Language
dc.contributor.authorDineshram, R.en
dc.contributor.authorQ., Quanen
dc.contributor.authorSharma, Rakeshen
dc.contributor.authorChandramouli, Kondethimmanahallien
dc.contributor.authorYalamanchili, Hari Krishnaen
dc.contributor.authorChu, Ivanen
dc.contributor.authorThiyagarajan, Vengatesenen
dc.date.accessioned2016-11-03T08:28:03Z-
dc.date.available2016-11-03T08:28:03Z-
dc.date.issued2015-10-28en
dc.identifier.citationDineshram R, Q. Q, Sharma R, Chandramouli K, Yalamanchili HK, et al. (2015) Comparative and quantitative proteomics reveal the adaptive strategies of oyster larvae to ocean acidification. PROTEOMICS 15: 4120–4134. Available: http://dx.doi.org/10.1002/pmic.201500198.en
dc.identifier.issn1615-9853en
dc.identifier.doi10.1002/pmic.201500198en
dc.identifier.urihttp://hdl.handle.net/10754/621385-
dc.description.abstract© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Decreasing pH due to anthropogenic CO2 inputs, called ocean acidification (OA), can make coastal environments unfavorable for oysters. This is a serious socioeconomical issue for China which supplies >70% of the world's edible oysters. Here, we present an iTRAQ-based protein profiling approach for the detection and quantification of proteome changes under OA in the early life stage of a commercially important oyster, Crassostrea hongkongensis. Availability of complete genome sequence for the pacific oyster (Crassostrea gigas) enabled us to confidently quantify over 1500 proteins in larval oysters. Over 7% of the proteome was altered in response to OA at pHNBS 7.6. Analysis of differentially expressed proteins and their associated functional pathways showed an upregulation of proteins involved in calcification, metabolic processes, and oxidative stress, each of which may be important in physiological adaptation of this species to OA. The downregulation of cytoskeletal and signal transduction proteins, on the other hand, might have impaired cellular dynamics and organelle development under OA. However, there were no significant detrimental effects in developmental processes such as metamorphic success. Implications of the differentially expressed proteins and metabolic pathways in the development of OA resistance in oyster larvae are discussed. The MS proteomics data have been deposited to the ProteomeXchange with identifiers PXD002138 (http://proteomecentral.proteomexchange.org/dataset/PXD002138).en
dc.description.sponsorshipWe are grateful to two anonymous reviewers and the editor who offered several constructive comments and criticisms on this article. The authors would like to thank Ziniu Yu and Shu Xiao of South China Sea Institute for Oceanology for providing the oysters and larval culture facilities used in our experiments. We thank Yun Lam (City University of Hong Kong) for providing various constructive comments during the proteomics data analysis and interpretation. This study was funded by three GRF grants from the HKSAR-RGC (grant numbers: 705511P, 705112P, and 17304914).en
dc.publisherWiley-Blackwellen
dc.subjectClimate changeen
dc.subjectCrassostrea hongkongensisen
dc.subjectITRAQen
dc.subjectMetamorphosisen
dc.subjectOcean acidificationen
dc.subjectOyster larvaeen
dc.titleComparative and quantitative proteomics reveal the adaptive strategies of oyster larvae to ocean acidificationen
dc.typeArticleen
dc.contributor.departmentBiological; Environmental Sciences & Engineering; King Abdullah University of Science and Technology; Thuwal Kingdom of Saudi Arabiaen
dc.identifier.journalPROTEOMICSen
dc.contributor.institutionThe Swire Institute of Marine Sciences and School of Biological Sciences; The University of Hong Kong; Hong Kong Special Administrative Region P. R. Chinaen
dc.contributor.institutionDepartment of Chemistry; The University of Hong Kong; Hong Kong Special Administrative Region P. R. Chinaen
dc.contributor.institutionDepartment of Biochemistry; L.K.S Faculty of Medicine; The University of Hong Kong; Hong Kong Special Administrative Region P. R. Chinaen
dc.contributor.institutionDepartment of Biology and Chemistry; City University of Hong Kong; Kowloon Tong Hong Kongen
dc.contributor.institutionDepartment of Molecular and Human Genetics; Baylor College of Medicine; Houston TX USAen
kaust.authorChandramouli, Kondethimmanahallien
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