Compatibility analysis of 3D printer resin for biological applications

Handle URI:
http://hdl.handle.net/10754/621269
Title:
Compatibility analysis of 3D printer resin for biological applications
Authors:
Sivashankar, Shilpa; Agambayev, Sumeyra; Alamoudi, Kholod; Buttner, Ulrich; Khashab, Niveen M. ( 0000-0003-2728-0666 ) ; Salama, Khaled N. ( 0000-0001-7742-1282 )
Abstract:
The salient features of microfluidics such as reduced cost, handling small sample and reagent volumes and less time required to fabricate the devices has inspired the present work. The incompatibility of three-dimensional printer resins in their native form and the method to improve their compatibility to many biological processes via surface modification are reported. The compatibility of the material to build microfluidic devices was evaluated in three different ways: (i) determining if the ultraviolet (UV) cured resin inhibits the polymerase chain reaction (PCR), i.e. testing devices for PCR compatibility; (ii) observing agglutination complex formed on the surface of the UV cured resin when anti-C-reactive protein (CRP) antibodies and CRP proteins were allowed to agglutinate; and (iii) by culturing human embryonic kidney cell line cells and testing for its attachment and viability. It is shown that only a few among four in its native form could be used for fabrication of microchannels and that had the least effect on biological molecules that could be used for PCR and protein interactions and cells, whereas the others were used after treating the surface. Importance in building lab-on-chip/micrototal analysis systems and organ-on-chip devices is found.
KAUST Department:
King Abdullah University of Science and Technology (KAUST), Saudi Arabia
Citation:
Sivashankar S, Agambayev S, Alamoudi K, Buttner U, Khashab N, et al. (2016) Compatibility analysis of 3D printer resin for biological applications . Micro & Nano Letters 11: 654–659. Available: http://dx.doi.org/10.1049/mnl.2016.0530.
Publisher:
Institution of Engineering and Technology (IET)
Journal:
Micro & Nano Letters
Issue Date:
30-Aug-2016
DOI:
10.1049/mnl.2016.0530
Type:
Article
ISSN:
1750-0443
Additional Links:
http://ieeexplore.ieee.org/document/7660890/
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorSivashankar, Shilpaen
dc.contributor.authorAgambayev, Sumeyraen
dc.contributor.authorAlamoudi, Kholoden
dc.contributor.authorButtner, Ulrichen
dc.contributor.authorKhashab, Niveen M.en
dc.contributor.authorSalama, Khaled N.en
dc.date.accessioned2016-11-03T07:43:53Z-
dc.date.available2016-11-03T07:43:53Z-
dc.date.issued2016-08-30en
dc.identifier.citationSivashankar S, Agambayev S, Alamoudi K, Buttner U, Khashab N, et al. (2016) Compatibility analysis of 3D printer resin for biological applications . Micro & Nano Letters 11: 654–659. Available: http://dx.doi.org/10.1049/mnl.2016.0530.en
dc.identifier.issn1750-0443en
dc.identifier.doi10.1049/mnl.2016.0530en
dc.identifier.urihttp://hdl.handle.net/10754/621269-
dc.description.abstractThe salient features of microfluidics such as reduced cost, handling small sample and reagent volumes and less time required to fabricate the devices has inspired the present work. The incompatibility of three-dimensional printer resins in their native form and the method to improve their compatibility to many biological processes via surface modification are reported. The compatibility of the material to build microfluidic devices was evaluated in three different ways: (i) determining if the ultraviolet (UV) cured resin inhibits the polymerase chain reaction (PCR), i.e. testing devices for PCR compatibility; (ii) observing agglutination complex formed on the surface of the UV cured resin when anti-C-reactive protein (CRP) antibodies and CRP proteins were allowed to agglutinate; and (iii) by culturing human embryonic kidney cell line cells and testing for its attachment and viability. It is shown that only a few among four in its native form could be used for fabrication of microchannels and that had the least effect on biological molecules that could be used for PCR and protein interactions and cells, whereas the others were used after treating the surface. Importance in building lab-on-chip/micrototal analysis systems and organ-on-chip devices is found.en
dc.publisherInstitution of Engineering and Technology (IET)en
dc.relation.urlhttp://ieeexplore.ieee.org/document/7660890/en
dc.rightsThis is an open access article published by the IET under the Creative Commons Attribution-NonCommercial-NoDerivs Licenseen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectbiological techniquesen
dc.subjectresinsen
dc.subjectmicrofluidicsen
dc.subjectbioMEMSen
dc.subjectmicrofabricationen
dc.subjectsurface treatmenten
dc.subjectcuringen
dc.subjectenzymesen
dc.subjectmolecular biophysicsen
dc.subjectbiochemistryen
dc.subjectkidneyen
dc.subjectcellular biophysicsen
dc.subjectorgan-on-chip devicesen
dc.subjectcompatibility analysisen
dc.subjectthree-dimensional printer resinsen
dc.subjectmicrofluidic devicesen
dc.subjectultraviolet cured resinen
dc.subjectpolymerase chain reactionen
dc.subjectPCR compatibilityen
dc.subjectagglutination complexen
dc.subjectUV cured resinen
dc.subjectantiC-reactive protein antibodiesen
dc.subjectCRP proteinsen
dc.subjecthuman embryonic kidney cell lineen
dc.subjectmicrochannel fabricationen
dc.subjectprotein interactionsen
dc.subjectlab-on-chip-micrototal analysisen
dc.titleCompatibility analysis of 3D printer resin for biological applicationsen
dc.typeArticleen
dc.contributor.departmentKing Abdullah University of Science and Technology (KAUST), Saudi Arabiaen
dc.identifier.journalMicro & Nano Lettersen
dc.eprint.versionAccepted manuscripten
kaust.authorSivashankar, Shilpaen
kaust.authorAgambayev, Sumeyraen
kaust.authorAlamoudi, Kholoden
kaust.authorButtner, Ulrichen
kaust.authorKhashab, Niveen M.en
kaust.authorSalama, Khaled N.en
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