Functionalized magnetic nanowires for chemical and magneto-mechanical induction of cancer cell death

Handle URI:
http://hdl.handle.net/10754/621257
Title:
Functionalized magnetic nanowires for chemical and magneto-mechanical induction of cancer cell death
Authors:
Martinez Banderas, Aldo Isaac ( 0000-0003-1707-5597 ) ; Aires, Antonio; Teran, Francisco J.; Perez, Jose E. ( 0000-0002-2206-0034 ) ; Cadenas, Jael F.; Alsharif, Nouf Adnan ( 0000-0003-4057-5936 ) ; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Cortajarena, Aitziber L.; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
Exploiting and combining different properties of nanomaterials is considered a potential route for next generation cancer therapies. Magnetic nanowires (NWs) have shown good biocompatibility and a high level of cellular internalization. We induced cancer cell death by combining the chemotherapeutic effect of doxorubicin (DOX)-functionalized iron NWs with the mechanical disturbance under a low frequency alternating magnetic field. (3-aminopropyl)triethoxysilane (APTES) and bovine serum albumin (BSA) were separately used for coating NWs allowing further functionalization with DOX. Internalization was assessed for both formulations by confocal reflection microscopy and inductively coupled plasma-mass spectrometry. From confocal analysis, BSA formulations demonstrated higher internalization and less agglomeration. The functionalized NWs generated a comparable cytotoxic effect in breast cancer cells in a DOX concentration-dependent manner, (~60% at the highest concentration tested) that was significantly different from the effect produced by free DOX and non-functionalized NWs formulations. A synergistic cytotoxic effect is obtained when a magnetic field (1 mT, 10 Hz) is applied to cells treated with DOX-functionalized BSA or APTES-coated NWs, (~70% at the highest concentration). In summary, a bimodal method for cancer cell destruction was developed by the conjugation of the magneto-mechanical properties of iron NWs with the effect of DOX producing better results than the individual effects.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Citation:
Martínez-Banderas AI, Aires A, Teran FJ, Perez JE, Cadenas JF, et al. (2016) Functionalized magnetic nanowires for chemical and magneto-mechanical induction of cancer cell death. Scientific Reports 6: 35786. Available: http://dx.doi.org/10.1038/srep35786.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
24-Oct-2016
DOI:
10.1038/srep35786
Type:
Article
ISSN:
2045-2322
Sponsors:
We thank Sylvia Gutiérrez Erlandsson (Confocal microscopy service CNB-CSIC) and Manuel Roldan Rodriguez (Imaging and Characterization Core Lab at KAUST). This work was partially supported by European Commission (MULTIFUN, no. 262943), Comunidad de Madrid NANOFRONTMAG-CM project (S2013/MIT-2850), and Spanish Ministry of Economy and Competitiveness (BIO2012-34835 and MAT2013-47395-C4-3-R). F.J.T. acknowledges the financial support from Ramon y Cajal subprogram (RYC-2011-09617). Research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://www.nature.com/articles/srep35786
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMartinez Banderas, Aldo Isaacen
dc.contributor.authorAires, Antonioen
dc.contributor.authorTeran, Francisco J.en
dc.contributor.authorPerez, Jose E.en
dc.contributor.authorCadenas, Jael F.en
dc.contributor.authorAlsharif, Nouf Adnanen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorCortajarena, Aitziber L.en
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2016-10-31T07:37:25Z-
dc.date.available2016-10-31T07:37:25Z-
dc.date.issued2016-10-24en
dc.identifier.citationMartínez-Banderas AI, Aires A, Teran FJ, Perez JE, Cadenas JF, et al. (2016) Functionalized magnetic nanowires for chemical and magneto-mechanical induction of cancer cell death. Scientific Reports 6: 35786. Available: http://dx.doi.org/10.1038/srep35786.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep35786en
dc.identifier.urihttp://hdl.handle.net/10754/621257-
dc.description.abstractExploiting and combining different properties of nanomaterials is considered a potential route for next generation cancer therapies. Magnetic nanowires (NWs) have shown good biocompatibility and a high level of cellular internalization. We induced cancer cell death by combining the chemotherapeutic effect of doxorubicin (DOX)-functionalized iron NWs with the mechanical disturbance under a low frequency alternating magnetic field. (3-aminopropyl)triethoxysilane (APTES) and bovine serum albumin (BSA) were separately used for coating NWs allowing further functionalization with DOX. Internalization was assessed for both formulations by confocal reflection microscopy and inductively coupled plasma-mass spectrometry. From confocal analysis, BSA formulations demonstrated higher internalization and less agglomeration. The functionalized NWs generated a comparable cytotoxic effect in breast cancer cells in a DOX concentration-dependent manner, (~60% at the highest concentration tested) that was significantly different from the effect produced by free DOX and non-functionalized NWs formulations. A synergistic cytotoxic effect is obtained when a magnetic field (1 mT, 10 Hz) is applied to cells treated with DOX-functionalized BSA or APTES-coated NWs, (~70% at the highest concentration). In summary, a bimodal method for cancer cell destruction was developed by the conjugation of the magneto-mechanical properties of iron NWs with the effect of DOX producing better results than the individual effects.en
dc.description.sponsorshipWe thank Sylvia Gutiérrez Erlandsson (Confocal microscopy service CNB-CSIC) and Manuel Roldan Rodriguez (Imaging and Characterization Core Lab at KAUST). This work was partially supported by European Commission (MULTIFUN, no. 262943), Comunidad de Madrid NANOFRONTMAG-CM project (S2013/MIT-2850), and Spanish Ministry of Economy and Competitiveness (BIO2012-34835 and MAT2013-47395-C4-3-R). F.J.T. acknowledges the financial support from Ramon y Cajal subprogram (RYC-2011-09617). Research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST).en
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.nature.com/articles/srep35786en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleFunctionalized magnetic nanowires for chemical and magneto-mechanical induction of cancer cell deathen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionIMDEA Nanociencia and Nanobiotechnology Unit associated to Centro Nacional de Biotecnología (CNB-CSIC), Campus Universitario de Cantoblanco, Madrid, 28049, Spainen
dc.contributor.institutionCIC BiomaGUNE, Parque Tecnológico de San Sebastián, Paseo Miramón 182, Donostia-San Sebastián 20009, Spainen
dc.contributor.institutionIkerbasque, Basque Foundation for Science, Mª Díaz de Haro 3, E-48013 Bilbao, Spainen
kaust.authorMartinez Banderas, Aldo Isaacen
kaust.authorPerez, Jose E.en
kaust.authorAlsharif, Nouf Adnanen
kaust.authorRavasi, Timothyen
kaust.authorKosel, Jürgenen
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