Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential

Handle URI:
http://hdl.handle.net/10754/621094
Title:
Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential
Authors:
Al Amoudi, Soha ( 0000-0003-1028-7634 ) ; Essack, Magbubah ( 0000-0003-2709-5356 ) ; Simões, Marta F.; Bougouffa, Salim ( 0000-0001-9218-6452 ) ; Soloviev, Irina; Archer, John A.C. ( 0000-0002-3302-3933 ) ; Lafi, Feras Fawzi; Bajic, Vladimir B. ( 0000-0001-5435-4750 )
Abstract:
Microorganisms that inhabit unchartered unique soil such as in the highly saline and hot Red Sea lagoons on the Saudi Arabian coastline, represent untapped sources of potentially new bioactive compounds. In this study, a culture-dependent approach was applied to three types of sediments: mangrove mud (MN), microbial mat (MM), and barren soil (BS), collected from Rabigh harbor lagoon (RHL) and Al-Kharrar lagoon (AKL). The isolated bacteria were evaluated for their potential to produce bioactive compounds. The phylogenetic characterization of 251 bacterial isolates based on the 16S rRNA gene sequencing, supported their assignment to five different phyla: Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, and Planctomycetes. Fifteen putative novel species were identified based on a 16S rRNA gene sequence similarity to other strain sequences in the NCBI database, being ≤98%. We demonstrate that 49 of the 251 isolates exhibit the potential to produce antimicrobial compounds. Additionally, at least one type of biosynthetic gene sequence, responsible for the synthesis of secondary metabolites, was recovered from 25 of the 49 isolates. Moreover, 10 of the isolates had a growth inhibition effect towards Staphylococcus aureus, Salmonella typhimurium and Pseudomonas syringae. We report the previously unknown antimicrobial activity of B. borstelensis, P. dendritiformis and M. salipaludis against all three indicator pathogens. Our study demonstrates the evidence of diverse cultured microbes associated with the Red Sea harbor/lagoon environments and their potential to produce antimicrobial compounds.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Citation:
Al-Amoudi S, Essack M, Simões M, Bougouffa S, Soloviev I, et al. (2016) Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential. Marine Drugs 14: 165. Available: http://dx.doi.org/10.3390/md14090165.
Publisher:
MDPI AG
Journal:
Marine Drugs
KAUST Grant Number:
URF/1/1976-02; FCS/1/2448-01; CCF URF 1976
Issue Date:
10-Sep-2016
DOI:
10.3390/md14090165
Type:
Article
ISSN:
1660-3397
Sponsors:
The authors wish to acknowledge the experimental support from the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory. The computational analysis for this study was performed on Dragon and Snapdragon compute clusters of the Computational Bioscience Research Center at KAUST. The authors would also like to thank Christoph Gehring and his research group for their help and use of their instruments and equipment. We also thank Andre Antunes for his support and advice. This publication is based upon work supported by the KAUST Office of Sponsored Research (OSR) under Awards No URF/1/1976-02 and FCS/1/2448-01, and KAUST base research funds to V.B.B. Funding for open access charge: King Abdullah University of Science and Technology (CCF URF 1976).
Additional Links:
http://www.mdpi.com/1660-3397/14/9/165
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorAl Amoudi, Sohaen
dc.contributor.authorEssack, Magbubahen
dc.contributor.authorSimões, Marta F.en
dc.contributor.authorBougouffa, Salimen
dc.contributor.authorSoloviev, Irinaen
dc.contributor.authorArcher, John A.C.en
dc.contributor.authorLafi, Feras Fawzien
dc.contributor.authorBajic, Vladimir B.en
dc.date.accessioned2016-10-20T11:52:12Z-
dc.date.available2016-10-20T11:52:12Z-
dc.date.issued2016-09-10en
dc.identifier.citationAl-Amoudi S, Essack M, Simões M, Bougouffa S, Soloviev I, et al. (2016) Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential. Marine Drugs 14: 165. Available: http://dx.doi.org/10.3390/md14090165.en
dc.identifier.issn1660-3397en
dc.identifier.doi10.3390/md14090165en
dc.identifier.urihttp://hdl.handle.net/10754/621094-
dc.description.abstractMicroorganisms that inhabit unchartered unique soil such as in the highly saline and hot Red Sea lagoons on the Saudi Arabian coastline, represent untapped sources of potentially new bioactive compounds. In this study, a culture-dependent approach was applied to three types of sediments: mangrove mud (MN), microbial mat (MM), and barren soil (BS), collected from Rabigh harbor lagoon (RHL) and Al-Kharrar lagoon (AKL). The isolated bacteria were evaluated for their potential to produce bioactive compounds. The phylogenetic characterization of 251 bacterial isolates based on the 16S rRNA gene sequencing, supported their assignment to five different phyla: Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, and Planctomycetes. Fifteen putative novel species were identified based on a 16S rRNA gene sequence similarity to other strain sequences in the NCBI database, being ≤98%. We demonstrate that 49 of the 251 isolates exhibit the potential to produce antimicrobial compounds. Additionally, at least one type of biosynthetic gene sequence, responsible for the synthesis of secondary metabolites, was recovered from 25 of the 49 isolates. Moreover, 10 of the isolates had a growth inhibition effect towards Staphylococcus aureus, Salmonella typhimurium and Pseudomonas syringae. We report the previously unknown antimicrobial activity of B. borstelensis, P. dendritiformis and M. salipaludis against all three indicator pathogens. Our study demonstrates the evidence of diverse cultured microbes associated with the Red Sea harbor/lagoon environments and their potential to produce antimicrobial compounds.en
dc.description.sponsorshipThe authors wish to acknowledge the experimental support from the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory. The computational analysis for this study was performed on Dragon and Snapdragon compute clusters of the Computational Bioscience Research Center at KAUST. The authors would also like to thank Christoph Gehring and his research group for their help and use of their instruments and equipment. We also thank Andre Antunes for his support and advice. This publication is based upon work supported by the KAUST Office of Sponsored Research (OSR) under Awards No URF/1/1976-02 and FCS/1/2448-01, and KAUST base research funds to V.B.B. Funding for open access charge: King Abdullah University of Science and Technology (CCF URF 1976).en
dc.publisherMDPI AGen
dc.relation.urlhttp://www.mdpi.com/1660-3397/14/9/165en
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/en
dc.subjectRed Seaen
dc.subjectmangrove muden
dc.subjectmicrobial maten
dc.subjectbarren soilen
dc.subjectculturable bacteriaen
dc.subjectphylogenetic diversityen
dc.subject16S rRNAen
dc.subjectfirmicutesen
dc.subjectbioactivityen
dc.subjectantimicrobial compoundsen
dc.subjectbiosynthetic genesen
dc.subjectbioinformaticsen
dc.titleBioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potentialen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalMarine Drugsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionBiology Department, Edge Hill University, St. Helens Road, Lancashire, Ormskirk L39 4QP, UKen
kaust.authorAl Amoudi, Sohaen
kaust.authorEssack, Magbubahen
kaust.authorSimões, Marta F.en
kaust.authorBougouffa, Salimen
kaust.authorSoloviev, Irinaen
kaust.authorArcher, John A.C.en
kaust.authorLafi, Feras Fawzien
kaust.authorBajic, Vladimir B.en
kaust.grant.numberURF/1/1976-02en
kaust.grant.numberFCS/1/2448-01en
kaust.grant.numberCCF URF 1976en
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