Sex Change in Clownfish: Molecular Insights from Transcriptome Analysis

Handle URI:
http://hdl.handle.net/10754/621062
Title:
Sex Change in Clownfish: Molecular Insights from Transcriptome Analysis
Authors:
Casas, Laura; Saborido-Rey, Fran; Ryu, Tae Woo; Michell, Craig; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Irigoien, Xabier ( 0000-0002-5411-6741 )
Abstract:
Sequential hermaphroditism is a unique reproductive strategy among teleosts that is displayed mainly in fish species living in the coral reef environment. The reproductive biology of hermaphrodites has long been intriguing; however, very little is known about the molecular pathways underlying their sex change. Here, we provide the first de novo transcriptome analyses of a hermaphrodite teleost´s undergoing sex change in its natural environment. Our study has examined relative gene expression across multiple groups—rather than just two contrasting conditions— and has allowed us to explore the differential expression patterns throughout the whole process. Our analysis has highlighted the rapid and complex genomic response of the brain associated with sex change, which is subsequently transmitted to the gonads, identifying a large number of candidate genes, some well-known and some novel, involved in the process. The present study provides strong evidence of the importance of the sex steroidogenic machinery during sex change in clownfish, with the aromatase gene playing a central role, both in the brain and the gonad. This work constitutes the first genome-wide study in a social sex-changing species and provides insights into the genetic mechanism governing social sex change and gonadal restructuring in protandrous hermaphrodites.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC); Applied Mathematics and Computational Science Program; KAUST Environmental Epigenetics Research Program (KEEP)
Citation:
Casas L, Saborido-Rey F, Ryu T, Michell C, Ravasi T, et al. (2016) Sex Change in Clownfish: Molecular Insights from Transcriptome Analysis. Scientific Reports 6: 35461. Available: http://dx.doi.org/10.1038/srep35461.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
17-Oct-2016
DOI:
10.1038/srep35461
Type:
Article
ISSN:
2045-2322
Sponsors:
The authors would like to thank the Coastal and Marine Resources Core Lab, particularly Dave H. Pallet, for their invaluable support during fieldwork. We would like to express our gratitude to Tane Sinclair-Taylor and Camrin Braun for finding and tagging the clownfish families used in the present study and Paula R. Tamargo and Sonia Rábade for their assistance on histological processing. We also acknowledge helpful comments and advice from Prof. Laszlo Orban. We wish to thank Virginia A. Unkefer from the KAUST Academic Writing Services who assisted in the proof-reading of the manuscript. Figure 6 is courtesy of Ivan Gromicho (Academic Writing Services, KAUST, Thuwal, Saudi Arabia).
Additional Links:
http://www.nature.com/articles/srep35461
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Applied Mathematics and Computational Science Program; Biological and Environmental Sciences and Engineering (BESE) Division; Reef Genomics, part of the Global Ocean Genome Project

Full metadata record

DC FieldValue Language
dc.contributor.authorCasas, Lauraen
dc.contributor.authorSaborido-Rey, Franen
dc.contributor.authorRyu, Tae Wooen
dc.contributor.authorMichell, Craigen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorIrigoien, Xabieren
dc.date.accessioned2016-10-19T08:05:17Z-
dc.date.available2016-10-19T08:05:17Z-
dc.date.issued2016-10-17en
dc.identifier.citationCasas L, Saborido-Rey F, Ryu T, Michell C, Ravasi T, et al. (2016) Sex Change in Clownfish: Molecular Insights from Transcriptome Analysis. Scientific Reports 6: 35461. Available: http://dx.doi.org/10.1038/srep35461.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep35461en
dc.identifier.urihttp://hdl.handle.net/10754/621062-
dc.description.abstractSequential hermaphroditism is a unique reproductive strategy among teleosts that is displayed mainly in fish species living in the coral reef environment. The reproductive biology of hermaphrodites has long been intriguing; however, very little is known about the molecular pathways underlying their sex change. Here, we provide the first de novo transcriptome analyses of a hermaphrodite teleost´s undergoing sex change in its natural environment. Our study has examined relative gene expression across multiple groups—rather than just two contrasting conditions— and has allowed us to explore the differential expression patterns throughout the whole process. Our analysis has highlighted the rapid and complex genomic response of the brain associated with sex change, which is subsequently transmitted to the gonads, identifying a large number of candidate genes, some well-known and some novel, involved in the process. The present study provides strong evidence of the importance of the sex steroidogenic machinery during sex change in clownfish, with the aromatase gene playing a central role, both in the brain and the gonad. This work constitutes the first genome-wide study in a social sex-changing species and provides insights into the genetic mechanism governing social sex change and gonadal restructuring in protandrous hermaphrodites.en
dc.description.sponsorshipThe authors would like to thank the Coastal and Marine Resources Core Lab, particularly Dave H. Pallet, for their invaluable support during fieldwork. We would like to express our gratitude to Tane Sinclair-Taylor and Camrin Braun for finding and tagging the clownfish families used in the present study and Paula R. Tamargo and Sonia Rábade for their assistance on histological processing. We also acknowledge helpful comments and advice from Prof. Laszlo Orban. We wish to thank Virginia A. Unkefer from the KAUST Academic Writing Services who assisted in the proof-reading of the manuscript. Figure 6 is courtesy of Ivan Gromicho (Academic Writing Services, KAUST, Thuwal, Saudi Arabia).en
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.nature.com/articles/srep35461en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleSex Change in Clownfish: Molecular Insights from Transcriptome Analysisen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.contributor.departmentKAUST Environmental Epigenetics Research Program (KEEP)en
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionInstitute of Marine Research (IIM-CSIC), 36208 Vigo, Spainen
kaust.authorCasas, Lauraen
kaust.authorRyu, Tae Wooen
kaust.authorMichell, Craigen
kaust.authorRavasi, Timothyen
kaust.authorIrigoien, Xabieren
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