An impaired metabolic response to hydrostatic pressure explains Alcanivorax borkumensis recorded distribution in the deep marine water column

Handle URI:
http://hdl.handle.net/10754/620937
Title:
An impaired metabolic response to hydrostatic pressure explains Alcanivorax borkumensis recorded distribution in the deep marine water column
Authors:
Scoma, Alberto; Barbato, Marta; Borin, Sara; Daffonchio, Daniele ( 0000-0003-0947-925X ) ; Boon, Nico
Abstract:
Alcanivorax borkumensis is an ubiquitous model organism for hydrocarbonoclastic bacteria, which dominates polluted surface waters. Its negligible presence in oil-contaminated deep waters (as observed during the Deepwater Horizon accident) raises the hypothesis that it may lack adaptive mechanisms to hydrostatic pressure (HP). The type strain SK2 was tested under 0.1, 5 and 10 MPa (corresponding to surface water, 500 and 1000 m depth, respectively). While 5 MPa essentially inactivated SK2, further increase to 10 MPa triggered some resistance mechanism, as indicated by higher total and intact cell numbers. Under 10 MPa, SK2 upregulated the synthetic pathway of the osmolyte ectoine, whose concentration increased from 0.45 to 4.71 fmoles cell-1. Central biosynthetic pathways such as cell replication, glyoxylate and Krebs cycles, amino acids metabolism and fatty acids biosynthesis, but not β-oxidation, were upregulated or unaffected at 10 MPa, although total cell number was remarkably lower with respect to 0.1 MPa. Concomitantly, expression of more than 50% of SK2 genes was downregulated, including genes related to ATP generation, respiration and protein translation. Thus, A. borkumensis lacks proper adaptation to HP but activates resistance mechanisms. These consist in poorly efficient biosynthetic rather than energy-yielding degradation-related pathways, and suggest that HP does represent a major driver for its distribution at deep-sea.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Biological and Environmental Sciences and Engineering Division
Citation:
Scoma A, Barbato M, Borin S, Daffonchio D, Boon N (2016) An impaired metabolic response to hydrostatic pressure explains Alcanivorax borkumensis recorded distribution in the deep marine water column. Scientific Reports 6: 31316. Available: http://dx.doi.org/10.1038/srep31316.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
12-Aug-2016
DOI:
10.1038/srep31316
Type:
Article
ISSN:
2045-2322
Sponsors:
This work was funded by FP-7 project Kill Spill (No. 312139, “Integrated Biotechnological Solutions for Combating Marine Oil Spills”) and by the Geconcentreerde Onderzoeksactie (GOA) of Ghent University (BOF15/GOA/006). The authors thank the support of King Abdullah University of Science and Technology (baseline research funds to D.D.).
Additional Links:
http://www.nature.com/articles/srep31316
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorScoma, Albertoen
dc.contributor.authorBarbato, Martaen
dc.contributor.authorBorin, Saraen
dc.contributor.authorDaffonchio, Danieleen
dc.contributor.authorBoon, Nicoen
dc.date.accessioned2016-10-12T09:15:13Z-
dc.date.available2016-10-12T09:15:13Z-
dc.date.issued2016-08-12en
dc.identifier.citationScoma A, Barbato M, Borin S, Daffonchio D, Boon N (2016) An impaired metabolic response to hydrostatic pressure explains Alcanivorax borkumensis recorded distribution in the deep marine water column. Scientific Reports 6: 31316. Available: http://dx.doi.org/10.1038/srep31316.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep31316en
dc.identifier.urihttp://hdl.handle.net/10754/620937-
dc.description.abstractAlcanivorax borkumensis is an ubiquitous model organism for hydrocarbonoclastic bacteria, which dominates polluted surface waters. Its negligible presence in oil-contaminated deep waters (as observed during the Deepwater Horizon accident) raises the hypothesis that it may lack adaptive mechanisms to hydrostatic pressure (HP). The type strain SK2 was tested under 0.1, 5 and 10 MPa (corresponding to surface water, 500 and 1000 m depth, respectively). While 5 MPa essentially inactivated SK2, further increase to 10 MPa triggered some resistance mechanism, as indicated by higher total and intact cell numbers. Under 10 MPa, SK2 upregulated the synthetic pathway of the osmolyte ectoine, whose concentration increased from 0.45 to 4.71 fmoles cell-1. Central biosynthetic pathways such as cell replication, glyoxylate and Krebs cycles, amino acids metabolism and fatty acids biosynthesis, but not β-oxidation, were upregulated or unaffected at 10 MPa, although total cell number was remarkably lower with respect to 0.1 MPa. Concomitantly, expression of more than 50% of SK2 genes was downregulated, including genes related to ATP generation, respiration and protein translation. Thus, A. borkumensis lacks proper adaptation to HP but activates resistance mechanisms. These consist in poorly efficient biosynthetic rather than energy-yielding degradation-related pathways, and suggest that HP does represent a major driver for its distribution at deep-sea.en
dc.description.sponsorshipThis work was funded by FP-7 project Kill Spill (No. 312139, “Integrated Biotechnological Solutions for Combating Marine Oil Spills”) and by the Geconcentreerde Onderzoeksactie (GOA) of Ghent University (BOF15/GOA/006). The authors thank the support of King Abdullah University of Science and Technology (baseline research funds to D.D.).en
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.nature.com/articles/srep31316en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleAn impaired metabolic response to hydrostatic pressure explains Alcanivorax borkumensis recorded distribution in the deep marine water columnen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentBiological and Environmental Sciences and Engineering Divisionen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionCenter of Microbial Ecology and Technology (CMET), University of Gent, Coupure Links 653, B 9000 Gent, Belgiumen
dc.contributor.institutionDepartment of Food, Environmental and Nutritional Sciences (DeFENS), University of Milano, Via Celoria 2, 20133, Milano, Italyen
kaust.authorDaffonchio, Danieleen
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