Targeted viral-mediated plant genome editing using crispr/cas9

Handle URI:
http://hdl.handle.net/10754/619789
Title:
Targeted viral-mediated plant genome editing using crispr/cas9
Authors:
Mahfouz, Magdy M. ( 0000-0002-0616-6365 ) ; Ali, Zahir ( 0000-0002-7814-8908 )
Assignee:
King Abdullah University Of Science And Technology
Abstract:
The present disclosure provides a viral-mediated genome-editing platform that facilitates multiplexing, obviates stable transformation, and is applicable across plant species. The RNA2 genome of the tobacco rattle virus (TRV) was engineered to carry and systemically deliver a guide RNA molecules into plants overexpressing Cas9 endonuclease. High genomic modification frequencies were observed in inoculated as well as systemic leaves including the plant growing points. This system facilitates multiplexing and can lead to germinal transmission of the genomic modifications in the progeny, thereby obviating the requirements of repeated transformations and tissue culture. The editing platform of the disclosure is useful in plant genome engineering and applicable across plant species amenable to viral infections for agricultural biotechnology applications.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Issue Date:
17-Dec-2015
Submitted date:
2014-06-19
Type:
Patent
Application Number:
WO 2015189693 A1
Patent Status:
Published Application
Additional Links:
http://www.google.com/patents/WO2015189693A1; http://worldwide.espacenet.com/publicationDetails/biblio?CC=WO&NR=2015189693A1&KC=A1&FT=D
Appears in Collections:
Patents

Full metadata record

DC FieldValue Language
dc.contributor.authorMahfouz, Magdy M.en
dc.contributor.authorAli, Zahiren
dc.date.accessioned2016-09-04T11:31:51Z-
dc.date.available2016-09-04T11:31:51Z-
dc.date.issued2015-12-17-
dc.date.submitted2014-06-19-
dc.identifier.urihttp://hdl.handle.net/10754/619789-
dc.description.abstractThe present disclosure provides a viral-mediated genome-editing platform that facilitates multiplexing, obviates stable transformation, and is applicable across plant species. The RNA2 genome of the tobacco rattle virus (TRV) was engineered to carry and systemically deliver a guide RNA molecules into plants overexpressing Cas9 endonuclease. High genomic modification frequencies were observed in inoculated as well as systemic leaves including the plant growing points. This system facilitates multiplexing and can lead to germinal transmission of the genomic modifications in the progeny, thereby obviating the requirements of repeated transformations and tissue culture. The editing platform of the disclosure is useful in plant genome engineering and applicable across plant species amenable to viral infections for agricultural biotechnology applications.en
dc.relation.urlhttp://www.google.com/patents/WO2015189693A1en
dc.relation.urlhttp://worldwide.espacenet.com/publicationDetails/biblio?CC=WO&NR=2015189693A1&KC=A1&FT=Den
dc.titleTargeted viral-mediated plant genome editing using crispr/cas9en
dc.typePatenten
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.description.statusPublished Applicationen
dc.contributor.assigneeKing Abdullah University Of Science And Technologyen
dc.description.countryWorld Intellectual Property Organization (WIPO)en
dc.identifier.applicationnumberWO 2015189693 A1en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.