Different Endosymbiotic Interactions in Two Hydra Species Reflect the Evolutionary History of Endosymbiosis

Handle URI:
http://hdl.handle.net/10754/619779
Title:
Different Endosymbiotic Interactions in Two Hydra Species Reflect the Evolutionary History of Endosymbiosis
Authors:
Ishikawa, Masakazu; Yuyama, Ikuko; Shimizu, Hiroshi; Nozawa, Masafumi; Ikeo, Kazuho; Gojobori, Takashi ( 0000-0001-7850-1743 )
Abstract:
Endosymbiosis is an important evolutionary event for organisms, and there is widespread interest in understanding the evolution of endosymbiosis establishment. Hydra is one of the most suitable organisms for studying the evolution of endosymbiosis. Within the genus Hydra, H. viridissima and H. vulgaris show endosymbiosis with green algae. Previous studies suggested that the endosymbiosis in H. vulgaris took place much more recently than that in H. viridissima, noting that the establishment of the interaction between H. vulgaris and its algae is not as stable as in H. viridissima. To investigate the on-going process of endosymbiosis, we first compared growth and tolerance to starvation in symbiotic and aposymbiotic polyps of both species. The results revealed that symbiotic H. viridissima had a higher growth rate and greater tolerance to starvation than aposymbiotic polyps. By contrast, growth of symbiotic H. vulgaris was identical to that of aposymbiotic polyps, and symbiotic H. vulgaris was less tolerant to starvation. Moreover, our gene expression analysis showed a pattern of differential gene expression in H. viridissima similar to that in other endosymbiotically established organisms, and contrary to that observed in H. vulgaris. We also showed that H. viridissima could cope with oxidative stress that caused damage, such as cell death, in H. vulgaris. These observations support the idea that oxidative stress related genes play an important role in the on-going process of endosymbiosis evolution. The different evolutionary stages of endosymbiosis studied here provide a deeper insight into the evolutionary processes occurring toward a stable endosymbiosis.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Citation:
Different Endosymbiotic Interactions in Two Hydra Species Reflect the Evolutionary History of Endosymbiosis 2016, 8 (7):2155 Genome Biology and Evolution
Journal:
Genome Biology and Evolution
Issue Date:
19-Jun-2016
DOI:
10.1093/gbe/evw142
Type:
Article
ISSN:
1759-6653
Sponsors:
The authors thank Ikuko Masujima, Takako Komatsu, and Tae Yamada for helping with Hydra spp. maintenance, and the collaborators of the Beijing Institute for Genomics (BGI) and the Bioscience Core Lab in King Abdullah University of Science and Technology (KAUST) for carrying out sequencing. The authors would also like to thank the ROIS National Institute of Genetics for the use of their NIG supercomputer. This work was supported by the Graduate University for Advanced Studies (SOKENDAI) to M.I. and by KAUST baseline fund to T.G.
Additional Links:
http://gbe.oxfordjournals.org/lookup/doi/10.1093/gbe/evw142
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DC FieldValue Language
dc.contributor.authorIshikawa, Masakazuen
dc.contributor.authorYuyama, Ikukoen
dc.contributor.authorShimizu, Hiroshien
dc.contributor.authorNozawa, Masafumien
dc.contributor.authorIkeo, Kazuhoen
dc.contributor.authorGojobori, Takashien
dc.date.accessioned2016-09-04T11:20:05Z-
dc.date.available2016-09-04T11:20:05Z-
dc.date.issued2016-06-19-
dc.identifier.citationDifferent Endosymbiotic Interactions in Two Hydra Species Reflect the Evolutionary History of Endosymbiosis 2016, 8 (7):2155 Genome Biology and Evolutionen
dc.identifier.issn1759-6653-
dc.identifier.doi10.1093/gbe/evw142-
dc.identifier.urihttp://hdl.handle.net/10754/619779-
dc.description.abstractEndosymbiosis is an important evolutionary event for organisms, and there is widespread interest in understanding the evolution of endosymbiosis establishment. Hydra is one of the most suitable organisms for studying the evolution of endosymbiosis. Within the genus Hydra, H. viridissima and H. vulgaris show endosymbiosis with green algae. Previous studies suggested that the endosymbiosis in H. vulgaris took place much more recently than that in H. viridissima, noting that the establishment of the interaction between H. vulgaris and its algae is not as stable as in H. viridissima. To investigate the on-going process of endosymbiosis, we first compared growth and tolerance to starvation in symbiotic and aposymbiotic polyps of both species. The results revealed that symbiotic H. viridissima had a higher growth rate and greater tolerance to starvation than aposymbiotic polyps. By contrast, growth of symbiotic H. vulgaris was identical to that of aposymbiotic polyps, and symbiotic H. vulgaris was less tolerant to starvation. Moreover, our gene expression analysis showed a pattern of differential gene expression in H. viridissima similar to that in other endosymbiotically established organisms, and contrary to that observed in H. vulgaris. We also showed that H. viridissima could cope with oxidative stress that caused damage, such as cell death, in H. vulgaris. These observations support the idea that oxidative stress related genes play an important role in the on-going process of endosymbiosis evolution. The different evolutionary stages of endosymbiosis studied here provide a deeper insight into the evolutionary processes occurring toward a stable endosymbiosis.en
dc.description.sponsorshipThe authors thank Ikuko Masujima, Takako Komatsu, and Tae Yamada for helping with Hydra spp. maintenance, and the collaborators of the Beijing Institute for Genomics (BGI) and the Bioscience Core Lab in King Abdullah University of Science and Technology (KAUST) for carrying out sequencing. The authors would also like to thank the ROIS National Institute of Genetics for the use of their NIG supercomputer. This work was supported by the Graduate University for Advanced Studies (SOKENDAI) to M.I. and by KAUST baseline fund to T.G.en
dc.language.isoenen
dc.relation.urlhttp://gbe.oxfordjournals.org/lookup/doi/10.1093/gbe/evw142en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen
dc.subjectendosymbiosisen
dc.subjecthydraen
dc.subjectRNA-seqen
dc.subjectdifferential gene expressionen
dc.subjecttolerance to starvationen
dc.subjectoxidative stressen
dc.titleDifferent Endosymbiotic Interactions in Two Hydra Species Reflect the Evolutionary History of Endosymbiosisen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalGenome Biology and Evolutionen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Genetics, SOKENDAI, Yata, Mishima, Shizuoka, Japanen
dc.contributor.institutionCenter for Information Biology, National Institute of Genetics, Yata, Mishima, Shizuoka, Japanen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorShimizu, Hiroshien
kaust.authorGojobori, Takashien
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