DNA Diagnostics: Optical or by Electronics?

Handle URI:
http://hdl.handle.net/10754/619778
Title:
DNA Diagnostics: Optical or by Electronics?
Authors:
Khan, Hadayat Ullah; Knoll, Wolfgang
Abstract:
In this paper, we very briefly review DNA biosensors based on optical and electrical detection principles, referring mainly to our past work applying both techniques but here using nearly identical sensor chip surface architectures, i.e., capture probe layers that were prepared based on a pulsed plasma deposition protocol for maleic anhydride and subsequent wet-chemical attachment of the amine-functionalized peptide nucleic acid (PNA) probe oligonucleotides. 15 mer DNA target strands, labeled with Cy5-chromophores that were attached at the 5’ end were used for surface plasmon optical detection and the same target DNA but without label was used in OTFT sensor-based detection where the mere charge density of the bound (hybridized) DNA molecules modulate the source-drain current. The sensing mechanisms and the detection limits of the devices are described in some detail. Both techniques allow for the monitoring of surface hybridization reactions, and offer the capacity to quantitatively discriminate between targets with different degrees of mismatched sequences.
KAUST Department:
Materials Science and Engineering (MSE); Physical Sciences and Engineering (PSE) Division
Citation:
DNA Diagnostics: Optical or by Electronics? 2016, 01 (s1) International Journal of Sensor Networks and Data Communications
Journal:
International Journal of Sensor Networks and Data Communications
Issue Date:
15-Jan-2016
DOI:
10.4172/2090-4886.S1-001
Type:
Article
ISSN:
20904886
Sponsors:
It is our great pleasure to acknowledge the collaboration and contribution of many colleagues to this work. In particular, we are grateful to Drs. Z. Bao, R. Corradini, R. Förch, D. Kambhampati, J.J. Kim, T. Liebermann, P.E. Nielsen, H. Park, M. Roberts, D. Yao, and F. Yu.
Additional Links:
http://www.omicsonline.com/open-access/dna-diagnostics-optical-or-by-electronics-2090-4886-S1-001.php?aid=67506
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorKhan, Hadayat Ullahen
dc.contributor.authorKnoll, Wolfgangen
dc.date.accessioned2016-09-04T11:18:02Z-
dc.date.available2016-09-04T11:18:02Z-
dc.date.issued2016-01-15-
dc.identifier.citationDNA Diagnostics: Optical or by Electronics? 2016, 01 (s1) International Journal of Sensor Networks and Data Communicationsen
dc.identifier.issn20904886-
dc.identifier.doi10.4172/2090-4886.S1-001-
dc.identifier.urihttp://hdl.handle.net/10754/619778-
dc.description.abstractIn this paper, we very briefly review DNA biosensors based on optical and electrical detection principles, referring mainly to our past work applying both techniques but here using nearly identical sensor chip surface architectures, i.e., capture probe layers that were prepared based on a pulsed plasma deposition protocol for maleic anhydride and subsequent wet-chemical attachment of the amine-functionalized peptide nucleic acid (PNA) probe oligonucleotides. 15 mer DNA target strands, labeled with Cy5-chromophores that were attached at the 5’ end were used for surface plasmon optical detection and the same target DNA but without label was used in OTFT sensor-based detection where the mere charge density of the bound (hybridized) DNA molecules modulate the source-drain current. The sensing mechanisms and the detection limits of the devices are described in some detail. Both techniques allow for the monitoring of surface hybridization reactions, and offer the capacity to quantitatively discriminate between targets with different degrees of mismatched sequences.en
dc.description.sponsorshipIt is our great pleasure to acknowledge the collaboration and contribution of many colleagues to this work. In particular, we are grateful to Drs. Z. Bao, R. Corradini, R. Förch, D. Kambhampati, J.J. Kim, T. Liebermann, P.E. Nielsen, H. Park, M. Roberts, D. Yao, and F. Yu.en
dc.language.isoenen
dc.relation.urlhttp://www.omicsonline.com/open-access/dna-diagnostics-optical-or-by-electronics-2090-4886-S1-001.php?aid=67506en
dc.rightsArchived with thanks to International Journal of Sensor Networks and Data Communications. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.titleDNA Diagnostics: Optical or by Electronics?en
dc.typeArticleen
dc.contributor.departmentMaterials Science and Engineering (MSE)en
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalInternational Journal of Sensor Networks and Data Communicationsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionThin Film Electronics AB, Westmansgatan 27, 58216, Linköping, Swedenen
dc.contributor.institutionAIT, Austrian Institute of Technology, Donau-City-Str. 1, 1220 Vienna, Austriaen
dc.contributor.institutionCenter for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University, Singaporeen
dc.contributor.institutionCenter for Electrochemical Surface Technology, Viktor-Kaplan-Straße 22700 Wiener Neustadt, Austriaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKhan, Hadayat Ullahen
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