Isolation and characterization of NDM-positive Escherichia coli from municipal wastewater in Jeddah, Saudi Arabia

Handle URI:
http://hdl.handle.net/10754/619771
Title:
Isolation and characterization of NDM-positive Escherichia coli from municipal wastewater in Jeddah, Saudi Arabia
Authors:
Mantilla-Calderon, David; Jumat, Raihan; Wang, Tiannyu; Ganesan, Pugalenthi; Aljassim, Nada I. ( 0000-0002-7667-2685 ) ; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
The emergence of resistance to last resort antibiotics is a public health concern of global scale. Besides direct person-to-person propagation, environmental pathways might contribute to the dissemination of antibiotic-resistant bacteria and antibiotic resistance genes (ARGs). Here, we describe the incidence of blaNDM-1, a gene conferring resistance to carbapenems, in the wastewater of Jeddah city over a one-year period. blaNDM-1 was detected at concentrations ranging from 104 to 105 copies per m3 of untreated wastewater during the entire monitoring period. These results indicate the ubiquity and high incidence of blaNDM-1 in the local wastewater. To track the bacteria carrying blaNDM-1, we isolated Escherichia coli PI7, a strain of the sequence type ST101, from wastewater around the Hajj event in October 2013. Genome sequencing of this strain revealed an extensive repertoire of ARGs as well as virulence and invasive traits. These traits were further confirmed by antibiotic resistance profiling and in-vitro cell internalization in HeLa cell cultures. Given that this strain remains viable even after a certain duration in the sewerage, and that Jeddah lacks a robust sanitary infrastructure to fully capture all generated sewage, the presence of this bacterium in the untreated wastewater would represent a potential hazard to the local public health. To the best of our knowledge, this is the first report of a blaNDM-1-positive E. coli isolated from a non-nosocomial environment in Saudi Arabia, and may set a preceding concern for the need to establish an improved surveillance for carbapenem-resistant E. coli in the country and nearby regions.
KAUST Department:
Water Desalination & Reuse Research Cntr; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Core Laboratory
Citation:
Isolation and characterization of NDM-positive Escherichia coli from municipal wastewater in Jeddah, Saudi Arabia 2016:AAC.00236-16 Antimicrobial Agents and Chemotherapy
Journal:
Antimicrobial Agents and Chemotherapy
Issue Date:
20-Jun-2016
DOI:
10.1128/AAC.00236-16
Type:
Article
ISSN:
0066-4804; 1098-6596
Sponsors:
The research reported in this publication was supported by funding BAS/1/1033-01-01 from King Abdullah University of Science and Technology (KAUST) awarded to P.-Y Hong.
Additional Links:
http://aac.asm.org/lookup/doi/10.1128/AAC.00236-16
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorMantilla-Calderon, Daviden
dc.contributor.authorJumat, Raihanen
dc.contributor.authorWang, Tiannyuen
dc.contributor.authorGanesan, Pugalenthien
dc.contributor.authorAljassim, Nada I.en
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2016-09-04T08:26:00Z-
dc.date.available2016-09-04T08:26:00Z-
dc.date.issued2016-06-20-
dc.identifier.citationIsolation and characterization of NDM-positive Escherichia coli from municipal wastewater in Jeddah, Saudi Arabia 2016:AAC.00236-16 Antimicrobial Agents and Chemotherapyen
dc.identifier.issn0066-4804-
dc.identifier.issn1098-6596-
dc.identifier.doi10.1128/AAC.00236-16-
dc.identifier.urihttp://hdl.handle.net/10754/619771-
dc.description.abstractThe emergence of resistance to last resort antibiotics is a public health concern of global scale. Besides direct person-to-person propagation, environmental pathways might contribute to the dissemination of antibiotic-resistant bacteria and antibiotic resistance genes (ARGs). Here, we describe the incidence of blaNDM-1, a gene conferring resistance to carbapenems, in the wastewater of Jeddah city over a one-year period. blaNDM-1 was detected at concentrations ranging from 104 to 105 copies per m3 of untreated wastewater during the entire monitoring period. These results indicate the ubiquity and high incidence of blaNDM-1 in the local wastewater. To track the bacteria carrying blaNDM-1, we isolated Escherichia coli PI7, a strain of the sequence type ST101, from wastewater around the Hajj event in October 2013. Genome sequencing of this strain revealed an extensive repertoire of ARGs as well as virulence and invasive traits. These traits were further confirmed by antibiotic resistance profiling and in-vitro cell internalization in HeLa cell cultures. Given that this strain remains viable even after a certain duration in the sewerage, and that Jeddah lacks a robust sanitary infrastructure to fully capture all generated sewage, the presence of this bacterium in the untreated wastewater would represent a potential hazard to the local public health. To the best of our knowledge, this is the first report of a blaNDM-1-positive E. coli isolated from a non-nosocomial environment in Saudi Arabia, and may set a preceding concern for the need to establish an improved surveillance for carbapenem-resistant E. coli in the country and nearby regions.en
dc.description.sponsorshipThe research reported in this publication was supported by funding BAS/1/1033-01-01 from King Abdullah University of Science and Technology (KAUST) awarded to P.-Y Hong.en
dc.language.isoenen
dc.relation.urlhttp://aac.asm.org/lookup/doi/10.1128/AAC.00236-16en
dc.rightsArchived with thanks to Antimicrobial Agents and Chemotherapy. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. http://creativecommons.org/licenses/by/4.0/en
dc.titleIsolation and characterization of NDM-positive Escherichia coli from municipal wastewater in Jeddah, Saudi Arabiaen
dc.typeArticleen
dc.contributor.departmentWater Desalination & Reuse Research Cntren
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Core Laboratoryen
dc.identifier.journalAntimicrobial Agents and Chemotherapyen
dc.eprint.versionPost-printen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorMantilla-Calderon, Daviden
kaust.authorJumat, Raihanen
kaust.authorWang, Tiannyuen
kaust.authorGanesan, Pugalenthien
kaust.authorAljassim, Nada I.en
kaust.authorHong, Pei-Yingen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.