SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl − accumulation and salt tolerance in Arabidopsis thaliana

Handle URI:
http://hdl.handle.net/10754/619766
Title:
SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl − accumulation and salt tolerance in Arabidopsis thaliana
Authors:
Qiu, Jiaen ( 0000-0001-9220-4219 ) ; Henderson, Sam W ( 0000-0003-3019-1891 ) ; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Roy, Stuart J; Gilliham, Mathew ( 0000-0003-0666-3078 )
Abstract:
Salinity tolerance is correlated with shoot chloride (Cl–) exclusion in multiple crops, but the molecular mechanisms of long-distance Cl– transport are poorly defined. Here, we characterize the in planta role of AtSLAH1 (a homologue of the slow type anion channel-associated 1 (SLAC1)). This protein, localized to the plasma membrane of root stelar cells, has its expression reduced by salt or ABA, which are key predictions for a protein involved with loading Cl– into the root xylem. Artificial microRNA knockdown mutants of AtSLAH1 had significantly reduced shoot Cl− accumulation when grown under low Cl–, whereas shoot Cl– increased and the shoot nitrate/chloride ratio decreased following AtSLAH1 constitutive or stelar-specific overexpression when grown in high Cl–. In both sets of overexpression lines a significant reduction in shoot biomass over the null segregants was observed under high Cl– supply, but not low Cl– supply. Further in planta data showed AtSLAH3 overexpression increased the shoot nitrate/chloride ratio, consistent with AtSLAH3 favouring nitrate transport. Heterologous expression of AtSLAH1 in Xenopus laevis oocytes led to no detectible transport, suggesting the need for post-translational modifications for AtSLAH1 to be active. Our in planta data are consistent with AtSLAH1 having a role in controlling root-to-shoot Cl– transport.
KAUST Department:
Center for Desert Agriculture
Citation:
SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl − accumulation and salt tolerance in Arabidopsis thaliana 2016, 67 (15):4495 Journal of Experimental Botany
Journal:
Journal of Experimental Botany
Issue Date:
23-Jun-2016
DOI:
10.1093/jxb/erw237
Type:
Article
ISSN:
0022-0957; 1460-2431
Sponsors:
The authors thank Dr Darren Plett for donating the destination vector (pTOOL5) in this study; Yuan Li and Hui Zhou for performing the qRT-PCRs (Australian Centre for Plant Function Genomics, Adelaide, Australia). The work was supported by: the Grains Research and Development Corporation (UA000145 to S.J.R., M.G.); the Australian Research Council (ARC) through Centre of Excellence (CE14010008) and Future Fellowship (FT130100709) funding to M.G.; and, the University of Adelaide Graduate Research Scholarship and the Australian Centre for Plant Functional Genomics student scholarship to J.Q.
Additional Links:
http://jxb.oxfordjournals.org/lookup/doi/10.1093/jxb/erw237
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Full metadata record

DC FieldValue Language
dc.contributor.authorQiu, Jiaenen
dc.contributor.authorHenderson, Sam Wen
dc.contributor.authorTester, Mark A.en
dc.contributor.authorRoy, Stuart Jen
dc.contributor.authorGilliham, Mathewen
dc.date.accessioned2016-09-04T08:06:13Z-
dc.date.available2016-09-04T08:06:13Z-
dc.date.issued2016-06-23-
dc.identifier.citationSLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl − accumulation and salt tolerance in Arabidopsis thaliana 2016, 67 (15):4495 Journal of Experimental Botanyen
dc.identifier.issn0022-0957-
dc.identifier.issn1460-2431-
dc.identifier.doi10.1093/jxb/erw237-
dc.identifier.urihttp://hdl.handle.net/10754/619766-
dc.description.abstractSalinity tolerance is correlated with shoot chloride (Cl–) exclusion in multiple crops, but the molecular mechanisms of long-distance Cl– transport are poorly defined. Here, we characterize the in planta role of AtSLAH1 (a homologue of the slow type anion channel-associated 1 (SLAC1)). This protein, localized to the plasma membrane of root stelar cells, has its expression reduced by salt or ABA, which are key predictions for a protein involved with loading Cl– into the root xylem. Artificial microRNA knockdown mutants of AtSLAH1 had significantly reduced shoot Cl− accumulation when grown under low Cl–, whereas shoot Cl– increased and the shoot nitrate/chloride ratio decreased following AtSLAH1 constitutive or stelar-specific overexpression when grown in high Cl–. In both sets of overexpression lines a significant reduction in shoot biomass over the null segregants was observed under high Cl– supply, but not low Cl– supply. Further in planta data showed AtSLAH3 overexpression increased the shoot nitrate/chloride ratio, consistent with AtSLAH3 favouring nitrate transport. Heterologous expression of AtSLAH1 in Xenopus laevis oocytes led to no detectible transport, suggesting the need for post-translational modifications for AtSLAH1 to be active. Our in planta data are consistent with AtSLAH1 having a role in controlling root-to-shoot Cl– transport.en
dc.description.sponsorshipThe authors thank Dr Darren Plett for donating the destination vector (pTOOL5) in this study; Yuan Li and Hui Zhou for performing the qRT-PCRs (Australian Centre for Plant Function Genomics, Adelaide, Australia). The work was supported by: the Grains Research and Development Corporation (UA000145 to S.J.R., M.G.); the Australian Research Council (ARC) through Centre of Excellence (CE14010008) and Future Fellowship (FT130100709) funding to M.G.; and, the University of Adelaide Graduate Research Scholarship and the Australian Centre for Plant Functional Genomics student scholarship to J.Q.en
dc.language.isoenen
dc.relation.urlhttp://jxb.oxfordjournals.org/lookup/doi/10.1093/jxb/erw237en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectABAen
dc.subjectArabidopsisen
dc.subjectAtSLAH1en
dc.subjectAtSLAH3en
dc.subjectchlorideen
dc.subjectCl− xylem loadingen
dc.subjectlong-distance transporten
dc.subjectnutritionen
dc.subjectsalinityen
dc.subjectslow-type anion channel-associated homologue 1en
dc.subjectslow-type anion channel-associated homologue 3en
dc.titleSLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl − accumulation and salt tolerance in Arabidopsis thalianaen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalJournal of Experimental Botanyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionSchool of Agriculture, Food, and Wine, University of Adelaide, PMB1, Glen Osmond, SA 5064, Australiaen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics, PMB1, Glen Osmond, SA 5064, Australiaen
dc.contributor.institutionARC Centre of Excellence in Plant Energy Biology, PMB1, Glen Osmond, SA 5064, Australiaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorTester, Mark A.en
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