Magnetotactic Bacterial Cages as Safe and Smart Gene Delivery Vehicles

Handle URI:
http://hdl.handle.net/10754/617788
Title:
Magnetotactic Bacterial Cages as Safe and Smart Gene Delivery Vehicles
Authors:
Alsaiari, Shahad K.; Ezzedine, Alaa H.; Abdallah, Abdallah; Sougrat, Rachid; Khashab, Niveen M. ( 0000-0003-2728-0666 )
Abstract:
In spite of the huge advances in the area of synthetic carriers, their efficiency still poorly compares to natural vectors. Herein, we report the use of unmodified magnetotactic bacteria as a guidable delivery vehicle for DNA functionalized gold nanoparticles (AuNPs). High cargo loading is established under anaerobic conditions (bacteria is alive) through endocytosis where AuNPs are employed as transmembrane proteins mimics (facilitate endocytosis) as well as imaging agents to verify and quantify loading and release. The naturally bio-mineralized magnetosomes, within the bacteria, induce heat generation inside bacteria through magnetic hyperthermia. Most importantly after exposing the system to air (bacteria is dead) the cell wall stays intact providing an efficient bacterial vessel. Upon incubation with THP-1 cells, the magnetotactic bacterial cages (MBCs) adhere to the cell wall and are directly engulfed through the phagocytic activity of these cells. Applying magnetic hyperthermia leads to the dissociation of the bacterial microcarrier and eventual release of cargo.
KAUST Department:
Smart Hybrid Materials (SHMs) lab; Advanced Membranes and Porous Materials Center (AMPMC); Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division; KAUST Advanced Nanofabrication Imaging and Characterization Core Laboratory
Citation:
Magnetotactic Bacterial Cages as Safe and Smart Gene Delivery Vehicles 2016 OpenNano
Publisher:
Elsevier BV
Journal:
OpenNano
Issue Date:
27-Jul-2016
DOI:
10.1016/j.onano.2016.07.001
Type:
Article
ISSN:
23529520
Sponsors:
The authors gratefully acknowledge King Abdullah University of Science and Technology (KAUST) for the support of this work. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S2352952016300093
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorAlsaiari, Shahad K.en
dc.contributor.authorEzzedine, Alaa H.en
dc.contributor.authorAbdallah, Abdallahen
dc.contributor.authorSougrat, Rachiden
dc.contributor.authorKhashab, Niveen M.en
dc.date.accessioned2016-08-01T10:47:06Z-
dc.date.available2016-08-01T10:47:06Z-
dc.date.issued2016-07-27-
dc.identifier.citationMagnetotactic Bacterial Cages as Safe and Smart Gene Delivery Vehicles 2016 OpenNanoen
dc.identifier.issn23529520-
dc.identifier.doi10.1016/j.onano.2016.07.001-
dc.identifier.urihttp://hdl.handle.net/10754/617788-
dc.description.abstractIn spite of the huge advances in the area of synthetic carriers, their efficiency still poorly compares to natural vectors. Herein, we report the use of unmodified magnetotactic bacteria as a guidable delivery vehicle for DNA functionalized gold nanoparticles (AuNPs). High cargo loading is established under anaerobic conditions (bacteria is alive) through endocytosis where AuNPs are employed as transmembrane proteins mimics (facilitate endocytosis) as well as imaging agents to verify and quantify loading and release. The naturally bio-mineralized magnetosomes, within the bacteria, induce heat generation inside bacteria through magnetic hyperthermia. Most importantly after exposing the system to air (bacteria is dead) the cell wall stays intact providing an efficient bacterial vessel. Upon incubation with THP-1 cells, the magnetotactic bacterial cages (MBCs) adhere to the cell wall and are directly engulfed through the phagocytic activity of these cells. Applying magnetic hyperthermia leads to the dissociation of the bacterial microcarrier and eventual release of cargo.en
dc.description.sponsorshipThe authors gratefully acknowledge King Abdullah University of Science and Technology (KAUST) for the support of this work. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S2352952016300093en
dc.rightsUnder a Creative Commons license, http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectmagnetotactic bacteriaen
dc.subjectmagnetosomesen
dc.subjectmicrobotsen
dc.subjecthyperthermiaen
dc.titleMagnetotactic Bacterial Cages as Safe and Smart Gene Delivery Vehiclesen
dc.typeArticleen
dc.contributor.departmentSmart Hybrid Materials (SHMs) laben
dc.contributor.departmentAdvanced Membranes and Porous Materials Center (AMPMC)en
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentKAUST Advanced Nanofabrication Imaging and Characterization Core Laboratoryen
dc.identifier.journalOpenNanoen
dc.eprint.versionPost-printen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAlsaiari, Shahad K.en
kaust.authorEzzedine, Alaa H.en
kaust.authorAbdallah, Abdallahen
kaust.authorSougrat, Rachiden
kaust.authorKhashab, Niveen M.en
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