LHP1 Regulates H3K27me3 Spreading and Shapes the Three-Dimensional Conformation of the Arabidopsis Genome

Handle URI:
http://hdl.handle.net/10754/617786
Title:
LHP1 Regulates H3K27me3 Spreading and Shapes the Three-Dimensional Conformation of the Arabidopsis Genome
Authors:
Veluchamy, Alaguraj; Jégu, Teddy; Ariel, Federico; Latrasse, David; Mariappan, Kiruthiga Gayathri; Kim, SoonKap; Crespi, Martin; Hirt, Heribert ( 0000-0003-3119-9633 ) ; Bergounioux, Catherine; Raynaud, Cécile; Benhamed, Moussa
Abstract:
Precise expression patterns of genes in time and space are essential for proper development of multicellular organisms. Dynamic chromatin conformation and spatial organization of the genome constitute a major step in this regulation to modulate developmental outputs. Polycomb repressive complexes (PRCs) mediate stable or flexible gene repression in response to internal and environmental cues. In Arabidopsis thaliana, LHP1 co-localizes with H3K27me3 epigenetic marks throughout the genome and interacts with PRC1 and PRC2 members as well as with a long noncoding RNA. Here, we show that LHP1 is responsible for the spreading of H3K27me3 towards the 3’ end of the gene body. We also identified a subset of LHP1-activated genes and demonstrated that LHP1 shapes local chromatin topology in order to control transcriptional co-regulation. Our work reveals a general role of LHP1 from local to higher conformation levels of chromatin configuration to determine its accessibility to define gene expression patterns.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
LHP1 Regulates H3K27me3 Spreading and Shapes the Three-Dimensional Conformation of the Arabidopsis Genome 2016, 11 (7):e0158936 PLOS ONE
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
Issue Date:
13-Jul-2016
DOI:
10.1371/journal.pone.0158936
Type:
Article
ISSN:
1932-6203
Sponsors:
TJ was Paris-Sud University PhD fellow. The IPS2 benefits from the support of the LabExSaclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS).
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0158936
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorVeluchamy, Alagurajen
dc.contributor.authorJégu, Teddyen
dc.contributor.authorAriel, Federicoen
dc.contributor.authorLatrasse, Daviden
dc.contributor.authorMariappan, Kiruthiga Gayathrien
dc.contributor.authorKim, SoonKapen
dc.contributor.authorCrespi, Martinen
dc.contributor.authorHirt, Heriberten
dc.contributor.authorBergounioux, Catherineen
dc.contributor.authorRaynaud, Cécileen
dc.contributor.authorBenhamed, Moussaen
dc.date.accessioned2016-08-01T10:30:55Z-
dc.date.available2016-08-01T10:30:55Z-
dc.date.issued2016-07-13-
dc.identifier.citationLHP1 Regulates H3K27me3 Spreading and Shapes the Three-Dimensional Conformation of the Arabidopsis Genome 2016, 11 (7):e0158936 PLOS ONEen
dc.identifier.issn1932-6203-
dc.identifier.doi10.1371/journal.pone.0158936-
dc.identifier.urihttp://hdl.handle.net/10754/617786-
dc.description.abstractPrecise expression patterns of genes in time and space are essential for proper development of multicellular organisms. Dynamic chromatin conformation and spatial organization of the genome constitute a major step in this regulation to modulate developmental outputs. Polycomb repressive complexes (PRCs) mediate stable or flexible gene repression in response to internal and environmental cues. In Arabidopsis thaliana, LHP1 co-localizes with H3K27me3 epigenetic marks throughout the genome and interacts with PRC1 and PRC2 members as well as with a long noncoding RNA. Here, we show that LHP1 is responsible for the spreading of H3K27me3 towards the 3’ end of the gene body. We also identified a subset of LHP1-activated genes and demonstrated that LHP1 shapes local chromatin topology in order to control transcriptional co-regulation. Our work reveals a general role of LHP1 from local to higher conformation levels of chromatin configuration to determine its accessibility to define gene expression patterns.en
dc.description.sponsorshipTJ was Paris-Sud University PhD fellow. The IPS2 benefits from the support of the LabExSaclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS).en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0158936en
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/en
dc.titleLHP1 Regulates H3K27me3 Spreading and Shapes the Three-Dimensional Conformation of the Arabidopsis Genomeen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPLoS ONEen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionInstitute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, University Paris-Sud, University of Evry, University Paris-Diderot, Sorbonne Paris-Cite, University of Paris-Saclay, Batiment 630, 91405, Orsay, Franceen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorVeluchamy, Alagurajen
kaust.authorMariappan, Kiruthiga Gayathrien
kaust.authorKim, SoonKapen
kaust.authorHirt, Heriberten
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.