Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans

Handle URI:
http://hdl.handle.net/10754/617521
Title:
Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans
Authors:
Gillis, Richard B.; Adams, Gary G.; Besong, Tabot M.D. ( 0000-0002-3572-1428 ) ; Machová, Eva; Ebringerová, Anna; Rowe, Arthur J.; Harding, Stephen E.; Patel, Trushar R.
Abstract:
Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently developed data analysis approaches were applied for traditional sedimentation equilibrium and velocity methods in order to investigate the molar mass distribution(s) of a subtype of polysaccharide, namely, mannans from four Candida spp. The molecular weight distributions of these mannans were studied using two recently developed equilibrium approaches: SEDFIT-MSTAR and MULTISIG, resulting in corroboratory distribution profiles. Additionally, sedimentation velocity data for all four mannans, analyzed using ls-g*(s) and Extended Fujita approaches, suggest that two of the fungal mannans (FM-1 and FM-3) have a unimodal distribution of molecular species whereas two others (FM-2 and FM-4) displayed bi-modal and broad distributions, respectively: this demonstrates considerable molecular heterogeneity in these polysaccharides, consistent with previous observations of mannans and polysaccharides in general. These methods not only have applications for the characterization of mannans but for other biopolymers such as polysaccharides, DNA, and proteins (including intrinsically disordered proteins).
KAUST Department:
Functional Nanomaterials Lab (FuNL)
Citation:
Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans 2016 European Biophysics Journal
Publisher:
Springer Nature
Journal:
European Biophysics Journal
Issue Date:
21-Jul-2016
DOI:
10.1007/s00249-016-1159-5
Type:
Article
ISSN:
0175-7571; 1432-1017
Sponsors:
TRP was in receipt of Developing Solutions PhD scholarship.
Additional Links:
http://link.springer.com/10.1007/s00249-016-1159-5
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorGillis, Richard B.en
dc.contributor.authorAdams, Gary G.en
dc.contributor.authorBesong, Tabot M.D.en
dc.contributor.authorMachová, Evaen
dc.contributor.authorEbringerová, Annaen
dc.contributor.authorRowe, Arthur J.en
dc.contributor.authorHarding, Stephen E.en
dc.contributor.authorPatel, Trushar R.en
dc.date.accessioned2016-07-26T09:18:46Z-
dc.date.available2016-07-26T09:18:46Z-
dc.date.issued2016-07-21-
dc.identifier.citationApplication of novel analytical ultracentrifuge analysis to solutions of fungal mannans 2016 European Biophysics Journalen
dc.identifier.issn0175-7571-
dc.identifier.issn1432-1017-
dc.identifier.doi10.1007/s00249-016-1159-5-
dc.identifier.urihttp://hdl.handle.net/10754/617521-
dc.description.abstractPolysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently developed data analysis approaches were applied for traditional sedimentation equilibrium and velocity methods in order to investigate the molar mass distribution(s) of a subtype of polysaccharide, namely, mannans from four Candida spp. The molecular weight distributions of these mannans were studied using two recently developed equilibrium approaches: SEDFIT-MSTAR and MULTISIG, resulting in corroboratory distribution profiles. Additionally, sedimentation velocity data for all four mannans, analyzed using ls-g*(s) and Extended Fujita approaches, suggest that two of the fungal mannans (FM-1 and FM-3) have a unimodal distribution of molecular species whereas two others (FM-2 and FM-4) displayed bi-modal and broad distributions, respectively: this demonstrates considerable molecular heterogeneity in these polysaccharides, consistent with previous observations of mannans and polysaccharides in general. These methods not only have applications for the characterization of mannans but for other biopolymers such as polysaccharides, DNA, and proteins (including intrinsically disordered proteins).en
dc.description.sponsorshipTRP was in receipt of Developing Solutions PhD scholarship.en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.urlhttp://link.springer.com/10.1007/s00249-016-1159-5en
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons. org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en
dc.subjectAUCen
dc.subjectExtended Fujita approachen
dc.subjectMULTISIGen
dc.subjectSEDFIT-MSTARen
dc.subjectSedimentationen
dc.titleApplication of novel analytical ultracentrifuge analysis to solutions of fungal mannansen
dc.typeArticleen
dc.contributor.departmentFunctional Nanomaterials Lab (FuNL)en
dc.identifier.journalEuropean Biophysics Journalen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionFaculty of Medicine and Health Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UKen
dc.contributor.institutionNational Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, Sutton Bonington LE12 5RD, UKen
dc.contributor.institutionCentre of Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Bratislava 84548, Slovakiaen
dc.contributor.institutionDepartment of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 4401 University Drive, Lethbridge, AB T1K 3M4, Canadaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorBesong, David T. M.en
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