Organic micropollutants in aerobic and anaerobic membrane bioreactors: Changes in microbial communities and gene expression

Handle URI:
http://hdl.handle.net/10754/617317
Title:
Organic micropollutants in aerobic and anaerobic membrane bioreactors: Changes in microbial communities and gene expression
Authors:
Harb, Moustapha ( 0000-0002-1566-987X ) ; Wei, Chunhai; Wang, Nan; Amy, Gary L.; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
Organic micro-pollutants (OMPs) are contaminants of emerging concern in wastewater treatment due to the risk of their proliferation into the environment, but their impact on the biological treatment process is not well understood. The purpose of this study is to examine the effects of the presence of OMPs on the core microbial populations of wastewater treatment. Two nanofiltration-coupled membrane bioreactors (aerobic and anaerobic) were subjected to the same operating conditions while treating synthetic municipal wastewater spiked with OMPs. Microbial community dynamics, gene expression levels, and antibiotic resistance genes were analyzed using molecular-based approaches. Results showed that presence of OMPs in the wastewater feed had a clear effect on keystone bacterial populations in both the aerobic and anaerobic sludge while also significantly impacting biodegradation-associated gene expression levels. Finally, multiple antibiotic-type OMPs were found to have higher removal rates in the anaerobic MBR, while associated antibiotic resistance genes were lower.
KAUST Department:
Water Desalination & Reuse Research Cntr; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Organic micropollutants in aerobic and anaerobic membrane bioreactors: Changes in microbial communities and gene expression 2016 Bioresource Technology
Publisher:
Elsevier BV
Journal:
Bioresource Technology
Issue Date:
9-Jul-2016
DOI:
10.1016/j.biortech.2016.07.036
Type:
Article
ISSN:
09608524
Sponsors:
The authors thank Y. Mao for providing the Perl scripts used for OTU sequence analysis and gene database information extraction. This study was partially funded by the KAUST Academic Partnership Program grant (PID 7000000105) and by the KAUST Center Competitive Funding Program grant (FCC/1/1971-06-01) awarded to P.-Y. Hong.
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0960852416310008
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Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorHarb, Moustaphaen
dc.contributor.authorWei, Chunhaien
dc.contributor.authorWang, Nanen
dc.contributor.authorAmy, Gary L.en
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2016-07-21T10:59:15Z-
dc.date.available2016-07-21T10:59:15Z-
dc.date.issued2016-07-09-
dc.identifier.citationOrganic micropollutants in aerobic and anaerobic membrane bioreactors: Changes in microbial communities and gene expression 2016 Bioresource Technologyen
dc.identifier.issn09608524-
dc.identifier.doi10.1016/j.biortech.2016.07.036-
dc.identifier.urihttp://hdl.handle.net/10754/617317-
dc.description.abstractOrganic micro-pollutants (OMPs) are contaminants of emerging concern in wastewater treatment due to the risk of their proliferation into the environment, but their impact on the biological treatment process is not well understood. The purpose of this study is to examine the effects of the presence of OMPs on the core microbial populations of wastewater treatment. Two nanofiltration-coupled membrane bioreactors (aerobic and anaerobic) were subjected to the same operating conditions while treating synthetic municipal wastewater spiked with OMPs. Microbial community dynamics, gene expression levels, and antibiotic resistance genes were analyzed using molecular-based approaches. Results showed that presence of OMPs in the wastewater feed had a clear effect on keystone bacterial populations in both the aerobic and anaerobic sludge while also significantly impacting biodegradation-associated gene expression levels. Finally, multiple antibiotic-type OMPs were found to have higher removal rates in the anaerobic MBR, while associated antibiotic resistance genes were lower.en
dc.description.sponsorshipThe authors thank Y. Mao for providing the Perl scripts used for OTU sequence analysis and gene database information extraction. This study was partially funded by the KAUST Academic Partnership Program grant (PID 7000000105) and by the KAUST Center Competitive Funding Program grant (FCC/1/1971-06-01) awarded to P.-Y. Hong.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0960852416310008en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Bioresource Technology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Bioresource Technology, 9 July 2016. DOI: 10.1016/j.biortech.2016.07.036en
dc.subjectTrace organic compoundsen
dc.subjectBioreactoren
dc.subjectTranscriptomicsen
dc.subjectAntibiotic resistance genesen
dc.subjectMBRen
dc.titleOrganic micropollutants in aerobic and anaerobic membrane bioreactors: Changes in microbial communities and gene expressionen
dc.typeArticleen
dc.contributor.departmentWater Desalination & Reuse Research Cntren
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalBioresource Technologyen
dc.eprint.versionPost-printen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorHarb, Moustaphaen
kaust.authorWei, Chunhaien
kaust.authorWang, Nanen
kaust.authorAmy, Gary L.en
kaust.authorHong, Pei-Yingen
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