Two-Step Evolution of Endosymbiosis between Hydra and Algae

Handle URI:
http://hdl.handle.net/10754/617129
Title:
Two-Step Evolution of Endosymbiosis between Hydra and Algae
Authors:
Ishikawa, Masakazu; Shimizu, Hiroshi; Nozawa, Masafumi; Ikeo, Kazuho; Gojobori, Takashi ( 0000-0001-7850-1743 )
Abstract:
In the Hydra vulgaris group, only 2 of the 25 strains in the collection of the National Institute of Genetics in Japan currently show endosymbiosis with green algae. However, whether the other non-symbiotic strains also have the potential to harbor algae remains unknown. The endosymbiotic potential of non-symbiotic strains that can harbor algae may have been acquired before or during divergence of the strains. With the aim of understanding the evolutionary process of endosymbiosis in the H. vulgaris group, we examined the endosymbiotic potential of non-symbiotic strains of the H. vulgaris group by artificially introducing endosymbiotic algae. We found that 12 of the 23 non-symbiotic strains were able to harbor the algae until reaching the grand-offspring through the asexual reproduction by budding. Moreover, a phylogenetic analysis of mitochondrial genome sequences showed that all the strains with endosymbiotic potential grouped into a single cluster (cluster γ). This cluster contained two strains (J7 and J10) that currently harbor algae; however, these strains were not the closest relatives. These results suggest that evolution of endosymbiosis occurred in two steps; first, endosymbiotic potential was gained once in the ancestor of the cluster γ lineage; second, strains J7 and J10 obtained algae independently after the divergence of the strains. By demonstrating the evolution of the endosymbiotic potential in non-symbiotic H. vulgaris group strains, we have clearly distinguished two evolutionary steps. The step-by-step evolutionary process provides significant insight into the evolution of endosymbiosis in cnidarians.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Citation:
Two-Step Evolution of Endosymbiosis between Hydra and Algae 2016 Molecular Phylogenetics and Evolution
Publisher:
Elsevier BV
Journal:
Molecular Phylogenetics and Evolution
Issue Date:
9-Jul-2016
DOI:
10.1016/j.ympev.2016.07.010
Type:
Article
ISSN:
10557903
Sponsors:
We thank Ikuko Masujima, Takako Komatsu, and Tae Yamada for helping to maintain the Hydra. We are also grateful to Chie Iwamoto for helping to run the Next Generation Sequencer and other experimental equipment.
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S1055790316301579
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorIshikawa, Masakazuen
dc.contributor.authorShimizu, Hiroshien
dc.contributor.authorNozawa, Masafumien
dc.contributor.authorIkeo, Kazuhoen
dc.contributor.authorGojobori, Takashien
dc.date.accessioned2016-07-18T09:23:23Z-
dc.date.available2016-07-18T09:23:23Z-
dc.date.issued2016-07-09-
dc.identifier.citationTwo-Step Evolution of Endosymbiosis between Hydra and Algae 2016 Molecular Phylogenetics and Evolutionen
dc.identifier.issn10557903-
dc.identifier.doi10.1016/j.ympev.2016.07.010-
dc.identifier.urihttp://hdl.handle.net/10754/617129-
dc.description.abstractIn the Hydra vulgaris group, only 2 of the 25 strains in the collection of the National Institute of Genetics in Japan currently show endosymbiosis with green algae. However, whether the other non-symbiotic strains also have the potential to harbor algae remains unknown. The endosymbiotic potential of non-symbiotic strains that can harbor algae may have been acquired before or during divergence of the strains. With the aim of understanding the evolutionary process of endosymbiosis in the H. vulgaris group, we examined the endosymbiotic potential of non-symbiotic strains of the H. vulgaris group by artificially introducing endosymbiotic algae. We found that 12 of the 23 non-symbiotic strains were able to harbor the algae until reaching the grand-offspring through the asexual reproduction by budding. Moreover, a phylogenetic analysis of mitochondrial genome sequences showed that all the strains with endosymbiotic potential grouped into a single cluster (cluster γ). This cluster contained two strains (J7 and J10) that currently harbor algae; however, these strains were not the closest relatives. These results suggest that evolution of endosymbiosis occurred in two steps; first, endosymbiotic potential was gained once in the ancestor of the cluster γ lineage; second, strains J7 and J10 obtained algae independently after the divergence of the strains. By demonstrating the evolution of the endosymbiotic potential in non-symbiotic H. vulgaris group strains, we have clearly distinguished two evolutionary steps. The step-by-step evolutionary process provides significant insight into the evolution of endosymbiosis in cnidarians.en
dc.description.sponsorshipWe thank Ikuko Masujima, Takako Komatsu, and Tae Yamada for helping to maintain the Hydra. We are also grateful to Chie Iwamoto for helping to run the Next Generation Sequencer and other experimental equipment.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S1055790316301579en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Molecular Phylogenetics and Evolution. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Molecular Phylogenetics and Evolution, 9 July 2016. DOI: 10.1016/j.ympev.2016.07.010en
dc.subjectHydraen
dc.subjectmitochondrial genomeen
dc.subjectendosymbiotic potentialen
dc.subjectChlorococcumen
dc.titleTwo-Step Evolution of Endosymbiosis between Hydra and Algaeen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalMolecular Phylogenetics and Evolutionen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Genetics, SOKENDAI, Yata, Mishima, Shizuoka 411-8540, Japanen
dc.contributor.institutionCenter for Information Biology, National Institute of Genetics, Yata, Mishima, Shizuoka 411-8540, Japanen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorShimizu, Hiroshien
kaust.authorGojobori, Takashien
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