A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss)

Handle URI:
http://hdl.handle.net/10754/615930
Title:
A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss)
Authors:
Khan, Uniza Wahid; Øverli, Øyvind; Hinkle, Patricia M.; Pasha, Farhan Ahmad; Johansen, Ida Beitnes; Berget, Ingunn; Silva, Patricia I. M.; Kittilsen, Silje; Höglund, Erik; Omholt, Stig W.; Våge, Dag Inge
Abstract:
In many vertebrate species visible melanin-based pigmentation patterns correlate with high stress- and disease-resistance, but proximate mechanisms for this trait association remain enigmatic. Here we show that a missense mutation in a classical pigmentation gene, melanocyte stimulating hormone receptor (MC1R), is strongly associated with distinct differences in steroidogenic melanocortin 2 receptor (MC2R) mRNA expression between high- (HR) and low-responsive (LR) rainbow trout (Oncorhynchus mykiss). We also show experimentally that cortisol implants increase the expression of agouti signaling protein (ASIP) mRNA in skin, likely explaining the association between HR-traits and reduced skin melanin patterning. Molecular dynamics simulations predict that melanocortin 2 receptor accessory protein (MRAP), needed for MC2R function, binds differently to the two MC1R variants. Considering that mRNA for MC2R and the MC1R variants are present in head kidney cells, we hypothesized that MC2R activity is modulated in part by different binding affinities of the MC1R variants for MRAP. Experiments in mammalian cells confirmed that trout MRAP interacts with the two trout MC1R variants and MC2R, but failed to detect regulation of MC2R signaling, possibly due to high constitutive MC1R activity.
KAUST Department:
KAUST Catalysis Center (KCC)
Citation:
A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) 2016, 6:28969 Scientific Reports
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
4-Jul-2016
DOI:
10.1038/srep28969
Type:
Article
ISSN:
2045-2322
Sponsors:
We are deeply thankful to Tom Pottinger for making the HR-LR rainbow trout strains available for this study. We thank Jon K. Lærdahl for making a structural model of MC1R, Christina Sørensen for helping with the cortisol analysis and Ida G. Lunde for help with the figures. Primer and competitor design for the real competitive PCR analysis was kindly performed by Sequenom Custom Service, Hamburg, Germany. The project was financially supported by the Norwegian University of Life Sciences, by Research Council of Norway projects no. 172609, 199728, 218534 and by National Institutes of Health Grant DK19974 (P.M.H.). Atlantic Salmon genomic sequences used for primer design was kindly provided by the International Cooperation to Sequence the Atlantic Salmon Genome (ICSASG).
Additional Links:
http://www.nature.com/articles/srep28969
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorKhan, Uniza Wahiden
dc.contributor.authorØverli, Øyvinden
dc.contributor.authorHinkle, Patricia M.en
dc.contributor.authorPasha, Farhan Ahmaden
dc.contributor.authorJohansen, Ida Beitnesen
dc.contributor.authorBerget, Ingunnen
dc.contributor.authorSilva, Patricia I. M.en
dc.contributor.authorKittilsen, Siljeen
dc.contributor.authorHöglund, Eriken
dc.contributor.authorOmholt, Stig W.en
dc.contributor.authorVåge, Dag Ingeen
dc.date.accessioned2016-07-11T09:56:19Z-
dc.date.available2016-07-11T09:56:19Z-
dc.date.issued2016-07-04-
dc.identifier.citationA novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) 2016, 6:28969 Scientific Reportsen
dc.identifier.issn2045-2322-
dc.identifier.doi10.1038/srep28969-
dc.identifier.urihttp://hdl.handle.net/10754/615930-
dc.description.abstractIn many vertebrate species visible melanin-based pigmentation patterns correlate with high stress- and disease-resistance, but proximate mechanisms for this trait association remain enigmatic. Here we show that a missense mutation in a classical pigmentation gene, melanocyte stimulating hormone receptor (MC1R), is strongly associated with distinct differences in steroidogenic melanocortin 2 receptor (MC2R) mRNA expression between high- (HR) and low-responsive (LR) rainbow trout (Oncorhynchus mykiss). We also show experimentally that cortisol implants increase the expression of agouti signaling protein (ASIP) mRNA in skin, likely explaining the association between HR-traits and reduced skin melanin patterning. Molecular dynamics simulations predict that melanocortin 2 receptor accessory protein (MRAP), needed for MC2R function, binds differently to the two MC1R variants. Considering that mRNA for MC2R and the MC1R variants are present in head kidney cells, we hypothesized that MC2R activity is modulated in part by different binding affinities of the MC1R variants for MRAP. Experiments in mammalian cells confirmed that trout MRAP interacts with the two trout MC1R variants and MC2R, but failed to detect regulation of MC2R signaling, possibly due to high constitutive MC1R activity.en
dc.description.sponsorshipWe are deeply thankful to Tom Pottinger for making the HR-LR rainbow trout strains available for this study. We thank Jon K. Lærdahl for making a structural model of MC1R, Christina Sørensen for helping with the cortisol analysis and Ida G. Lunde for help with the figures. Primer and competitor design for the real competitive PCR analysis was kindly performed by Sequenom Custom Service, Hamburg, Germany. The project was financially supported by the Norwegian University of Life Sciences, by Research Council of Norway projects no. 172609, 199728, 218534 and by National Institutes of Health Grant DK19974 (P.M.H.). Atlantic Salmon genomic sequences used for primer design was kindly provided by the International Cooperation to Sequence the Atlantic Salmon Genome (ICSASG).en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.nature.com/articles/srep28969en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleA novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss)en
dc.typeArticleen
dc.contributor.departmentKAUST Catalysis Center (KCC)en
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionCentre for Integrative Genetics, Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, NO-1430 Ås, Norwayen
dc.contributor.institutionUniversity of Rochester Medical Center, School of Medicine and Dentistry, Department of Pharmacology and Physiology, Rochester, NY 14642, USAen
dc.contributor.institutionDepartment of Molecular Biosciences, University of Oslo, NO-0316 Oslo, Norwayen
dc.contributor.institutionNorwegian School of Veterinary Medicine, Institute of Basal Sciences and Aquatic Medicine, NO- 0033 Oslo, Norwayen
dc.contributor.institutionTechnical University of Denmark, National Institute of Aquatic Resources, DK-9850 Hirtshals, Denmarken
dc.contributor.institutionNorwegian Institute for Water Research (NIVA), NO-0349 Oslo, Norwayen
dc.contributor.institutionNTNU - Norwegian University of Science and Technology, NO-7491 Trondheim, Norwayen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorPasha, Farhan Ahmaden
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