Characterization of Skeletonema costatum Intracellular Organic Matter and Study of Nanomechanical Properties under Different Solution Conditions

Handle URI:
http://hdl.handle.net/10754/614815
Title:
Characterization of Skeletonema costatum Intracellular Organic Matter and Study of Nanomechanical Properties under Different Solution Conditions
Authors:
Gutierrez, Leonardo ( 0000-0001-7573-6635 ) ; Aubry, Cyril; Dramas, Laure ( 0000-0002-8883-2084 ) ; Aimar, Pierre; Croue, Jean-Philippe
Abstract:
In the current investigation, a rigorous characterization of the high molecular weight (HMW) compounds of Skeletonema costatum (SKC) intracellular organic matter (IOM), including nanomechanical properties, was conducted. HMW SKC-IOM was characterized as a mixture of polysaccharides, proteins, and lipids. Atomic force microscopy (AFM) provided crucial information of this isolate at a nanoscale resolution. HMW SKC-IOM showed highly responsive to solution chemistry: fully extended chains at low ionic strength, and compressing structures with increasing electrolyte concentration in solution. Interestingly, two regions of different nanomechanical properties were observed: (a) Region #1: located farther from the substrate and showing extended polymeric chains, and (b) Region #2: located <10 nm above the substrate and presenting compressed structures. The polymer length, polymer grafting density, and compressibility of these two regions were highly influenced by solution conditions. Results suggest that steric interactions originating from HMW SKC-IOM polymeric structure would be a dominant interacting mechanism with surfaces. The current investigation has successfully applied models of polymer physics to describe the complex HMW SKC-IOM structural conformation at different solution conditions. The detailed methodology presented provides a tool to characterize and understand biopolymers interactions with surfaces, including filtration membranes, and can be extended to other environmentally relevant organic compounds.
KAUST Department:
Water Desalination & Reuse Research Cntr
Citation:
Characterization of Skeletonema costatum Intracellular Organic Matter and Study of Nanomechanical Properties under Different Solution Conditions 2016 Colloids and Surfaces A: Physicochemical and Engineering Aspects
Publisher:
Elsevier BV
Journal:
Colloids and Surfaces A: Physicochemical and Engineering Aspects
Issue Date:
17-Jun-2016
DOI:
10.1016/j.colsurfa.2016.06.025
Type:
Article
ISSN:
09277757
Sponsors:
The authors are grateful to the funding from King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0927775716304678
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorGutierrez, Leonardoen
dc.contributor.authorAubry, Cyrilen
dc.contributor.authorDramas, Laureen
dc.contributor.authorAimar, Pierreen
dc.contributor.authorCroue, Jean-Philippeen
dc.date.accessioned2016-06-27T10:21:11Z-
dc.date.available2016-06-27T10:21:11Z-
dc.date.issued2016-06-17-
dc.identifier.citationCharacterization of Skeletonema costatum Intracellular Organic Matter and Study of Nanomechanical Properties under Different Solution Conditions 2016 Colloids and Surfaces A: Physicochemical and Engineering Aspectsen
dc.identifier.issn09277757-
dc.identifier.doi10.1016/j.colsurfa.2016.06.025-
dc.identifier.urihttp://hdl.handle.net/10754/614815-
dc.description.abstractIn the current investigation, a rigorous characterization of the high molecular weight (HMW) compounds of Skeletonema costatum (SKC) intracellular organic matter (IOM), including nanomechanical properties, was conducted. HMW SKC-IOM was characterized as a mixture of polysaccharides, proteins, and lipids. Atomic force microscopy (AFM) provided crucial information of this isolate at a nanoscale resolution. HMW SKC-IOM showed highly responsive to solution chemistry: fully extended chains at low ionic strength, and compressing structures with increasing electrolyte concentration in solution. Interestingly, two regions of different nanomechanical properties were observed: (a) Region #1: located farther from the substrate and showing extended polymeric chains, and (b) Region #2: located <10 nm above the substrate and presenting compressed structures. The polymer length, polymer grafting density, and compressibility of these two regions were highly influenced by solution conditions. Results suggest that steric interactions originating from HMW SKC-IOM polymeric structure would be a dominant interacting mechanism with surfaces. The current investigation has successfully applied models of polymer physics to describe the complex HMW SKC-IOM structural conformation at different solution conditions. The detailed methodology presented provides a tool to characterize and understand biopolymers interactions with surfaces, including filtration membranes, and can be extended to other environmentally relevant organic compounds.en
dc.description.sponsorshipThe authors are grateful to the funding from King Abdullah University of Science and Technology (KAUST).en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0927775716304678en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Colloids and Surfaces A: Physicochemical and Engineering Aspects. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces A: Physicochemical and Engineering Aspects, 17 June 2016. DOI: 10.1016/j.colsurfa.2016.06.025en
dc.subjectAtomic force microscopyen
dc.subjectOrganic matteren
dc.subjectnanomechanical propertiesen
dc.subjectSkeletonema Costatumen
dc.subjectInterfacial interactionsen
dc.titleCharacterization of Skeletonema costatum Intracellular Organic Matter and Study of Nanomechanical Properties under Different Solution Conditionsen
dc.typeArticleen
dc.contributor.departmentWater Desalination & Reuse Research Cntren
dc.identifier.journalColloids and Surfaces A: Physicochemical and Engineering Aspectsen
dc.eprint.versionPost-printen
dc.contributor.institutionCurtin Water Quality Research Centre, Department of Chemistry, Curtin University, Australiaen
dc.contributor.institutionFacultad del Mar y Medio Ambiente, Universidad del Pacifico, Guayaquil, Ecuadoren
dc.contributor.institutionMasdar Institute of Science and Technology, Abu Dhabi, United Arab Emiratesen
dc.contributor.institutionUniversity of Toulouse, Toulouse, Franceen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDramas, Laureen
kaust.authorCroue, Jean-Philippeen
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