Metatranscriptomics reveals the molecular mechanism of large granule formation in granular anammox reactor

Handle URI:
http://hdl.handle.net/10754/614405
Title:
Metatranscriptomics reveals the molecular mechanism of large granule formation in granular anammox reactor
Authors:
Bagchi, Samik; Lamendella, Regina; Strutt, Steven; Van Loosdrecht, Mark C. M.; Saikaly, Pascal ( 0000-0001-7678-3986 )
Abstract:
Granules enriched with anammox bacteria are essential in enhancing the treatment of ammonia-rich wastewater, but little is known about how anammox bacteria grow and multiply inside granules. Here, we combined metatranscriptomics, quantitative PCR and 16S rRNA gene sequencing to study the changes in community composition, metabolic gene content and gene expression in a granular anammox reactor with the objective of understanding the molecular mechanism of anammox growth and multiplication that led to formation of large granules. Size distribution analysis revealed the spatial distribution of granules in which large granules having higher abundance of anammox bacteria (genus Brocadia) dominated the bottom biomass. Metatranscriptomics analysis detected all the essential transcripts for anammox metabolism. During the later stage of reactor operation, higher expression of ammonia and nitrite transport proteins and key metabolic enzymes mainly in the bottom large granules facilitated anammox bacteria activity. The high activity resulted in higher growth and multiplication of anammox bacteria and expanded the size of the granules. This conceptual model for large granule formation proposed here may assist in the future design of anammox processes for mainstream wastewater treatment.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination & Reuse Research Cntr
Citation:
Metatranscriptomics reveals the molecular mechanism of large granule formation in granular anammox reactor 2016, 6:28327 Scientific Reports
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
20-Jun-2016
DOI:
10.1038/srep28327
Type:
Article
ISSN:
2045-2322
Sponsors:
This research was funded by Competitive Research Grant (CRG_R2_13_SAIK_KAUST_1) from King Abdullah University of Science and Technology (KAUST). Special thanks are extended to Shan Sun for assisting with the qPCR analysis and Academic Writing Service Team at KAUST for making illustration figure and animation. We also thank Shahjahan Ali and colleagues in the Bioscience Core Laboratory at KAUST for their support in metatranscriptome sequencing on the Illumina HiSeq and in 16S rRNA gene sequencing on the Ion Torrent PGM. A portion of the bioinformatics analyses was supported by a grant to Juniata College from the Howard Hughes Medical Institute (http://www.hhmi.org) through the Precollege and Undergraduate Science Education Program.
Additional Links:
http://www.nature.com/articles/srep28327
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorBagchi, Samiken
dc.contributor.authorLamendella, Reginaen
dc.contributor.authorStrutt, Stevenen
dc.contributor.authorVan Loosdrecht, Mark C. M.en
dc.contributor.authorSaikaly, Pascalen
dc.date.accessioned2016-06-23T10:40:40Z-
dc.date.available2016-06-23T10:40:40Z-
dc.date.issued2016-06-20-
dc.identifier.citationMetatranscriptomics reveals the molecular mechanism of large granule formation in granular anammox reactor 2016, 6:28327 Scientific Reportsen
dc.identifier.issn2045-2322-
dc.identifier.doi10.1038/srep28327-
dc.identifier.urihttp://hdl.handle.net/10754/614405-
dc.description.abstractGranules enriched with anammox bacteria are essential in enhancing the treatment of ammonia-rich wastewater, but little is known about how anammox bacteria grow and multiply inside granules. Here, we combined metatranscriptomics, quantitative PCR and 16S rRNA gene sequencing to study the changes in community composition, metabolic gene content and gene expression in a granular anammox reactor with the objective of understanding the molecular mechanism of anammox growth and multiplication that led to formation of large granules. Size distribution analysis revealed the spatial distribution of granules in which large granules having higher abundance of anammox bacteria (genus Brocadia) dominated the bottom biomass. Metatranscriptomics analysis detected all the essential transcripts for anammox metabolism. During the later stage of reactor operation, higher expression of ammonia and nitrite transport proteins and key metabolic enzymes mainly in the bottom large granules facilitated anammox bacteria activity. The high activity resulted in higher growth and multiplication of anammox bacteria and expanded the size of the granules. This conceptual model for large granule formation proposed here may assist in the future design of anammox processes for mainstream wastewater treatment.en
dc.description.sponsorshipThis research was funded by Competitive Research Grant (CRG_R2_13_SAIK_KAUST_1) from King Abdullah University of Science and Technology (KAUST). Special thanks are extended to Shan Sun for assisting with the qPCR analysis and Academic Writing Service Team at KAUST for making illustration figure and animation. We also thank Shahjahan Ali and colleagues in the Bioscience Core Laboratory at KAUST for their support in metatranscriptome sequencing on the Illumina HiSeq and in 16S rRNA gene sequencing on the Ion Torrent PGM. A portion of the bioinformatics analyses was supported by a grant to Juniata College from the Howard Hughes Medical Institute (http://www.hhmi.org) through the Precollege and Undergraduate Science Education Program.en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.nature.com/articles/srep28327en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleMetatranscriptomics reveals the molecular mechanism of large granule formation in granular anammox reactoren
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination & Reuse Research Cntren
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionJuniata College, Biology Department, Huntingdon, PA 16652, USAen
dc.contributor.institutionDelft University of Technology, Environmental Biotechnology, Department of Biotechnology, Van der Maasweg 9, 2629 HZ Delft, The Netherlandsen
dc.contributor.institutionUniversity of Kansas, Department of Civil, Environmental, and Architectural Engineering, Lawrence, KS, USA 66045.en
dc.contributor.institutionUniversity of California, Berkeley, Department of Molecular and Cell Biology, Berkeley, CA 94720, USA.en
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorBagchi, Samiken
kaust.authorSaikaly, Pascalen
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