Integration of Fractal Biosensor in a Digital Microfluidic Platform

Handle URI:
http://hdl.handle.net/10754/613009
Title:
Integration of Fractal Biosensor in a Digital Microfluidic Platform
Authors:
Mashraei, Yousof; Sivashankar, Shilpa; Buttner, Ulrich; Salama, Khaled N. ( 0000-0001-7742-1282 )
Abstract:
The digital microfluidic (DMF) platform introduces many applications in biomedical assays. If it is to be commercially available to the public, it needs to have the essential features of smart sensing and a compact size. In this work, we report on a fractal electrode biosensor that is used for both droplet actuation and sensing C-reactive protein (CRP) concentration levels to assess cardiac disease risk. Our proposed electrode is the first two-terminal electrode design to be integrated into DMF platforms. A simulation of the electrical field distribution shows reduced peak intensities and uniform distribution of the field. When compared to a V-notch square electrode, the fractal electrode shows a superior performance in both aspects, i.e. field uniformity and intensity. These improvements are translated into a successful and responsive actuation of a water droplet with 100V. Likewise, the effective dielectric strength is improved by a 33% increase in the fractal electrode breakdown voltage. Additionally, the capability of the fractal electrode to work as a capacitive biosensor is evaluated with CRP quantification test. Selected fractal electrodes undergo a surface treatment to immobilize anti-CRP antibodies on their surface. The measurement shows a response to the added CRP in capacitance within three minutes. When the untreated electrodes were used for quantification, there was no significant change in capacitance, and this suggested that immobilization was necessary. The electrodes configuration in the fabricated DMF platform allows the fractal electrodes to be selectively used as biosensors, which means the device could be integrated into point-of-care applications.
KAUST Department:
Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division
Citation:
Integration of Fractal Biosensor in a Digital Microfluidic Platform 2016:1 IEEE Sensors Journal
Publisher:
Institute of Electrical and Electronics Engineers (IEEE)
Journal:
IEEE Sensors Journal
Issue Date:
8-Jun-2016
DOI:
10.1109/JSEN.2016.2578440
Type:
Article
ISSN:
1530-437X; 1558-1748; 2379-9153
Additional Links:
http://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=7487036
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorMashraei, Yousofen
dc.contributor.authorSivashankar, Shilpaen
dc.contributor.authorButtner, Ulrichen
dc.contributor.authorSalama, Khaled N.en
dc.date.accessioned2016-06-14T09:06:17Z-
dc.date.available2016-06-14T09:06:17Z-
dc.date.issued2016-06-08-
dc.identifier.citationIntegration of Fractal Biosensor in a Digital Microfluidic Platform 2016:1 IEEE Sensors Journalen
dc.identifier.issn1530-437X-
dc.identifier.issn1558-1748-
dc.identifier.issn2379-9153-
dc.identifier.doi10.1109/JSEN.2016.2578440-
dc.identifier.urihttp://hdl.handle.net/10754/613009-
dc.description.abstractThe digital microfluidic (DMF) platform introduces many applications in biomedical assays. If it is to be commercially available to the public, it needs to have the essential features of smart sensing and a compact size. In this work, we report on a fractal electrode biosensor that is used for both droplet actuation and sensing C-reactive protein (CRP) concentration levels to assess cardiac disease risk. Our proposed electrode is the first two-terminal electrode design to be integrated into DMF platforms. A simulation of the electrical field distribution shows reduced peak intensities and uniform distribution of the field. When compared to a V-notch square electrode, the fractal electrode shows a superior performance in both aspects, i.e. field uniformity and intensity. These improvements are translated into a successful and responsive actuation of a water droplet with 100V. Likewise, the effective dielectric strength is improved by a 33% increase in the fractal electrode breakdown voltage. Additionally, the capability of the fractal electrode to work as a capacitive biosensor is evaluated with CRP quantification test. Selected fractal electrodes undergo a surface treatment to immobilize anti-CRP antibodies on their surface. The measurement shows a response to the added CRP in capacitance within three minutes. When the untreated electrodes were used for quantification, there was no significant change in capacitance, and this suggested that immobilization was necessary. The electrodes configuration in the fabricated DMF platform allows the fractal electrodes to be selectively used as biosensors, which means the device could be integrated into point-of-care applications.en
dc.language.isoenen
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en
dc.relation.urlhttp://ieeexplore.ieee.org/lpdocs/epic03/wrapper.htm?arnumber=7487036en
dc.rights(c) 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works.en
dc.subjectC-reactive proteinen
dc.subjectdigital microfluidicsen
dc.subjectelectrowetting on dielectricen
dc.subjectfractal capacitoren
dc.titleIntegration of Fractal Biosensor in a Digital Microfluidic Platformen
dc.typeArticleen
dc.contributor.departmentComputer, Electrical and Mathematical Science and Engineering (CEMSE) Divisionen
dc.identifier.journalIEEE Sensors Journalen
dc.eprint.versionPost-printen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorMashraei, Yousofen
kaust.authorSivashankar, Shilpaen
kaust.authorButtner, Ulrichen
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