High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii

Handle URI:
http://hdl.handle.net/10754/611779
Title:
High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii
Authors:
Michoud, Gregoire ( 0000-0003-1071-9900 ) ; Jebbar, Mohamed
Abstract:
Pyrococcus yayanosii CH1, as the first and only obligate piezophilic hyperthermophilic microorganism discovered to date, extends the physical and chemical limits of life on Earth. It was isolated from the Ashadze hydrothermal vent at 4,100 m depth. Multi-omics analyses were performed to study the mechanisms used by the cell to cope with high hydrostatic pressure variations. In silico analyses showed that the P. yayanosii genome is highly adapted to its harsh environment, with a loss of aromatic amino acid biosynthesis pathways and the high constitutive expression of the energy metabolism compared with other non-obligate piezophilic Pyrococcus species. Differential proteomics and transcriptomics analyses identified key hydrostatic pressure-responsive genes involved in translation, chemotaxis, energy metabolism (hydrogenases and formate metabolism) and Clustered Regularly Interspaced Short Palindromic Repeats sequences associated with Cellular apoptosis susceptibility proteins.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii 2016, 6:27289 Scientific Reports
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
2-Jun-2016
DOI:
10.1038/srep27289
Type:
Article
ISSN:
2045-2322
Sponsors:
The authors would like to thank Didier Chevret for performing the proteomic analysis and Mickael Beauverger for help with protein extraction and gel migration. We are indebted to Helen McCombie for her helpful language improvement. This work was supported by the Agence Nationale de la Recherche (ANR-10-BLAN-1725 01-Living deep) and G.M. was supported by a PhD fellowship from the Conseil Régional de Bretagne.
Additional Links:
http://www.nature.com/articles/srep27289
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Articles

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DC FieldValue Language
dc.contributor.authorMichoud, Gregoireen
dc.contributor.authorJebbar, Mohameden
dc.date.accessioned2016-06-06T07:30:13Z-
dc.date.available2016-06-06T07:30:13Z-
dc.date.issued2016-06-02-
dc.identifier.citationHigh hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii 2016, 6:27289 Scientific Reportsen
dc.identifier.issn2045-2322-
dc.identifier.doi10.1038/srep27289-
dc.identifier.urihttp://hdl.handle.net/10754/611779-
dc.description.abstractPyrococcus yayanosii CH1, as the first and only obligate piezophilic hyperthermophilic microorganism discovered to date, extends the physical and chemical limits of life on Earth. It was isolated from the Ashadze hydrothermal vent at 4,100 m depth. Multi-omics analyses were performed to study the mechanisms used by the cell to cope with high hydrostatic pressure variations. In silico analyses showed that the P. yayanosii genome is highly adapted to its harsh environment, with a loss of aromatic amino acid biosynthesis pathways and the high constitutive expression of the energy metabolism compared with other non-obligate piezophilic Pyrococcus species. Differential proteomics and transcriptomics analyses identified key hydrostatic pressure-responsive genes involved in translation, chemotaxis, energy metabolism (hydrogenases and formate metabolism) and Clustered Regularly Interspaced Short Palindromic Repeats sequences associated with Cellular apoptosis susceptibility proteins.en
dc.description.sponsorshipThe authors would like to thank Didier Chevret for performing the proteomic analysis and Mickael Beauverger for help with protein extraction and gel migration. We are indebted to Helen McCombie for her helpful language improvement. This work was supported by the Agence Nationale de la Recherche (ANR-10-BLAN-1725 01-Living deep) and G.M. was supported by a PhD fellowship from the Conseil Régional de Bretagne.en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.nature.com/articles/srep27289en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleHigh hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosiien
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionUniv Brest, CNRS, Ifremer, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d’Urville, 29 280 Plouzané, Franceen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorMichoud, Gregoireen
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