Synthetic strategies for plant signalling studies: molecular toolbox and orthogonal platforms

Handle URI:
http://hdl.handle.net/10754/611237
Title:
Synthetic strategies for plant signalling studies: molecular toolbox and orthogonal platforms
Authors:
Braguy, Justine; Zurbriggen, Matias D.
Abstract:
Plants deploy a wide array of signalling networks integrating environmental cues with growth, defence and developmental responses. The high level of complexity, redundancy and connection between several pathways hampers a comprehensive understanding of involved functional and regulatory mechanisms. The implementation of synthetic biology approaches is revolutionizing experimental biology in prokaryotes, yeasts and animal systems and can likewise contribute to a new era in plant biology. This review gives an overview on synthetic biology approaches for the development and implementation of synthetic molecular tools and techniques to interrogate, understand and control signalling events in plants, ranging from strategies for the targeted manipulation of plant genomes up to the spatiotemporally resolved control of gene expression using optogenetic approaches. We also describe strategies based on the partial reconstruction of signalling pathways in orthogonal platforms, like yeast, animal and in vitro systems. This allows a targeted analysis of individual signalling hubs devoid of inter-connectivity with endogenous interacting components. Implementation of the interdisciplinary synthetic biology tools and strategies is not exempt of challenges and hardships but simultaneously most rewarding in terms of the advances in basic and applied research. As witnessed in other areas, these original theoretical-experimental avenues will lead to a breakthrough in the ability to study and comprehend plant signalling networks. This article is protected by copyright. All rights reserved.
KAUST Department:
King Abdullah University of Science and Technology (KAUST)
Citation:
Synthetic strategies for plant signalling studies: molecular toolbox and orthogonal platforms 2016 The Plant Journal
Publisher:
Wiley-Blackwell
Journal:
The Plant Journal
Issue Date:
26-May-2016
DOI:
10.1111/tpj.13218
Type:
Article
ISSN:
09607412
Sponsors:
We are indebted to Prof. Nestor Carrillo, Sophia L. Samodelov, Rocio Ochoa-Fernandez and Romina Sellaro for fruitful discussions and comments on the manuscript. We apologize to our colleagues whose work could not be cited due to space constraints. This work was supported by the Deutsche Forschungsgemeinschaft (DFG, EXC 1028) and the Heinrich-Heine-Universität Düsseldorf, Germany.
Additional Links:
http://doi.wiley.com/10.1111/tpj.13218
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorBraguy, Justineen
dc.contributor.authorZurbriggen, Matias D.en
dc.date.accessioned2016-05-31T12:11:53Z-
dc.date.available2016-05-31T12:11:53Z-
dc.date.issued2016-05-26-
dc.identifier.citationSynthetic strategies for plant signalling studies: molecular toolbox and orthogonal platforms 2016 The Plant Journalen
dc.identifier.issn09607412-
dc.identifier.doi10.1111/tpj.13218-
dc.identifier.urihttp://hdl.handle.net/10754/611237-
dc.description.abstractPlants deploy a wide array of signalling networks integrating environmental cues with growth, defence and developmental responses. The high level of complexity, redundancy and connection between several pathways hampers a comprehensive understanding of involved functional and regulatory mechanisms. The implementation of synthetic biology approaches is revolutionizing experimental biology in prokaryotes, yeasts and animal systems and can likewise contribute to a new era in plant biology. This review gives an overview on synthetic biology approaches for the development and implementation of synthetic molecular tools and techniques to interrogate, understand and control signalling events in plants, ranging from strategies for the targeted manipulation of plant genomes up to the spatiotemporally resolved control of gene expression using optogenetic approaches. We also describe strategies based on the partial reconstruction of signalling pathways in orthogonal platforms, like yeast, animal and in vitro systems. This allows a targeted analysis of individual signalling hubs devoid of inter-connectivity with endogenous interacting components. Implementation of the interdisciplinary synthetic biology tools and strategies is not exempt of challenges and hardships but simultaneously most rewarding in terms of the advances in basic and applied research. As witnessed in other areas, these original theoretical-experimental avenues will lead to a breakthrough in the ability to study and comprehend plant signalling networks. This article is protected by copyright. All rights reserved.en
dc.description.sponsorshipWe are indebted to Prof. Nestor Carrillo, Sophia L. Samodelov, Rocio Ochoa-Fernandez and Romina Sellaro for fruitful discussions and comments on the manuscript. We apologize to our colleagues whose work could not be cited due to space constraints. This work was supported by the Deutsche Forschungsgemeinschaft (DFG, EXC 1028) and the Heinrich-Heine-Universität Düsseldorf, Germany.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://doi.wiley.com/10.1111/tpj.13218en
dc.rightsThis is the peer reviewed version of the following article: Braguy, J. and Zurbriggen, M. D. (2016), Synthetic strategies for plant signalling studies: molecular toolbox and orthogonal platforms. Plant J. Accepted Author Manuscript., which has been published in final form at http://doi.wiley.com/10.1111/tpj.13218. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.titleSynthetic strategies for plant signalling studies: molecular toolbox and orthogonal platformsen
dc.typeArticleen
dc.contributor.departmentKing Abdullah University of Science and Technology (KAUST)en
dc.identifier.journalThe Plant Journalen
dc.eprint.versionPost-printen
dc.contributor.institutionInstitute of Synthetic Biology and CEPLAS; University of Düsseldorf; Universitätstrasse 1, Building 26.12.U1.25 40225 Düsseldorf Germanyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorBraguy, Justineen
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