An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity

Handle URI:
http://hdl.handle.net/10754/609439
Title:
An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity
Authors:
Lin, Xiao-Li; Niu, De; Hu, Zi-Liang; Kim, Dae Heon; Jin, Yin Hua; Cai, Bin; Liu, Peng; Miura, Kenji; Yun, Dae-Jin; Kim, Woe-Yeon; Lin, Rongcheng; Jin, Jing Bo ( 0000-0001-5847-5138 )
Abstract:
COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1), a ubiquitin E3 ligase, is a central negative regulator of photomorphogenesis. However, how COP1 activity is regulated by post-translational modifications remains largely unknown. Here we show that SUMO (small ubiquitin-like modifier) modification enhances COP1 activity. Loss-of-function siz1 mutant seedlings exhibit a weak constitutive photomorphogenic phenotype. SIZ1 physically interacts with COP1 and mediates the sumoylation of COP1. A K193R substitution in COP1 blocks its SUMO modification and reduces COP1 activity in vitro and in planta. Consistently, COP1 activity is reduced in siz1 and the level of HY5, a COP1 target protein, is increased in siz1. Sumoylated COP1 may exhibits higher transubiquitination activity than does non-sumoylated COP1, but SIZ1-mediated SUMO modification does not affect COP1 dimerization, COP1-HY5 interaction, and nuclear accumulation of COP1. Interestingly, prolonged light exposure reduces the sumoylation level of COP1, and COP1 mediates the ubiquitination and degradation of SIZ1. These regulatory mechanisms may maintain the homeostasis of COP1 activity, ensuing proper photomorphogenic development in changing light environment. Our genetic and biochemical studies identify a function for SIZ1 in photomorphogenesis and reveal a novel SUMO-regulated ubiquitin ligase, COP1, in plants.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity 2016, 12 (4):e1006016 PLOS Genetics
Publisher:
Public Library of Science (PLoS)
Journal:
PLOS Genetics
Issue Date:
29-Apr-2016
DOI:
10.1371/journal.pgen.1006016
Type:
Article
ISSN:
1553-7404
Sponsors:
This work was supported by grants 31170170 and 31471363, from the National Natural Science Foundation of China (http://www.nsfc.gov.cn/), to JBJ. This work was also supported by grant 2012CB114302, from the Ministry of Science and Technology of the People’s Republic of China (http://www.most.gov.cn/eng/), and by grant XDA08010105, from the Chinese Academy of Sciences (http://www.cas.cn/), to JBJ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Additional Links:
http://dx.plos.org/10.1371/journal.pgen.1006016
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLin, Xiao-Lien
dc.contributor.authorNiu, Deen
dc.contributor.authorHu, Zi-Liangen
dc.contributor.authorKim, Dae Heonen
dc.contributor.authorJin, Yin Huaen
dc.contributor.authorCai, Binen
dc.contributor.authorLiu, Pengen
dc.contributor.authorMiura, Kenjien
dc.contributor.authorYun, Dae-Jinen
dc.contributor.authorKim, Woe-Yeonen
dc.contributor.authorLin, Rongchengen
dc.contributor.authorJin, Jing Boen
dc.date.accessioned2016-05-15T13:32:03Zen
dc.date.available2016-05-15T13:32:03Zen
dc.date.issued2016-04-29en
dc.identifier.citationAn Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity 2016, 12 (4):e1006016 PLOS Geneticsen
dc.identifier.issn1553-7404en
dc.identifier.doi10.1371/journal.pgen.1006016en
dc.identifier.urihttp://hdl.handle.net/10754/609439en
dc.description.abstractCOP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1), a ubiquitin E3 ligase, is a central negative regulator of photomorphogenesis. However, how COP1 activity is regulated by post-translational modifications remains largely unknown. Here we show that SUMO (small ubiquitin-like modifier) modification enhances COP1 activity. Loss-of-function siz1 mutant seedlings exhibit a weak constitutive photomorphogenic phenotype. SIZ1 physically interacts with COP1 and mediates the sumoylation of COP1. A K193R substitution in COP1 blocks its SUMO modification and reduces COP1 activity in vitro and in planta. Consistently, COP1 activity is reduced in siz1 and the level of HY5, a COP1 target protein, is increased in siz1. Sumoylated COP1 may exhibits higher transubiquitination activity than does non-sumoylated COP1, but SIZ1-mediated SUMO modification does not affect COP1 dimerization, COP1-HY5 interaction, and nuclear accumulation of COP1. Interestingly, prolonged light exposure reduces the sumoylation level of COP1, and COP1 mediates the ubiquitination and degradation of SIZ1. These regulatory mechanisms may maintain the homeostasis of COP1 activity, ensuing proper photomorphogenic development in changing light environment. Our genetic and biochemical studies identify a function for SIZ1 in photomorphogenesis and reveal a novel SUMO-regulated ubiquitin ligase, COP1, in plants.en
dc.description.sponsorshipThis work was supported by grants 31170170 and 31471363, from the National Natural Science Foundation of China (http://www.nsfc.gov.cn/), to JBJ. This work was also supported by grant 2012CB114302, from the Ministry of Science and Technology of the People’s Republic of China (http://www.most.gov.cn/eng/), and by grant XDA08010105, from the Chinese Academy of Sciences (http://www.cas.cn/), to JBJ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pgen.1006016en
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/en
dc.titleAn Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activityen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPLOS Geneticsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, Chinaen
dc.contributor.institutionDepartment of Biology, Sunchon National University, Sunchon, Republic of Koreaen
dc.contributor.institutionFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japanen
dc.contributor.institutionDivision of Applied Life Science (BK21Plus), PMBBRC & IALS, Gyeongsang National University, Jinju, Republic of Koreaen
dc.contributor.institutionKey Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, Chinaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
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