Investigation and Isolation of Cellulase-Producing microorganisms in the Red Sea

Handle URI:
http://hdl.handle.net/10754/609149
Title:
Investigation and Isolation of Cellulase-Producing microorganisms in the Red Sea
Authors:
Fatani, Siham ( 0000-0002-4992-9502 )
Abstract:
Cellulolytic microorganisms are considered to be key players in biorefinery, especially for the utilization of plant biomass. These organisms have been isolated from various environments. The Red Sea is one of the seas with high biodiversity and a unique environment, characterized by high water temperature and high salinity . However, there is little information regarding cellulases in Red Sea environments. The aim of the present study is to evaluate the Red Sea as a gene resource for microbial cellulase. I first surveyed microbial cellulases in the Red Sea using a method called metagenomes, and then investigated their abundance and diversity. My survey revealed that the Red Sea biome has a substantial abundance and a wide range of cellulase enzymes with substantial abundance, when compared with those in other environments. Next, I tried to isolate cellulase-active microorganisms from the Red Sea and I successfully obtained seven strains of four different taxonomic groups. These strains showed a similarity of 99% identity to Aspergillus ustus, 99% to Staphylococcus pasteuri, 99% to Bacillus aerius and 99% to Bacillus subtilis. The enzyme assay I conducted, revealed that these strains actually secreted active cellulases. These results suggest that the Red Sea environment can be, indeed, an excellent gene resource of microbial cellulases.
Advisors:
Gojobori, Takashi ( 0000-0001-7850-1743 )
Committee Member:
Arold, Stefan T. ( 0000-0001-5278-0668 ) ; Mineta, Katsuhiko ( 0000-0002-4727-045X )
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience
Program:
Bioscience
Issue Date:
May-2016
Type:
Thesis
Appears in Collections:
Theses; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.advisorGojobori, Takashien
dc.contributor.authorFatani, Sihamen
dc.date.accessioned2016-05-12T07:04:24Zen
dc.date.available2016-05-12T07:04:24Zen
dc.date.issued2016-05en
dc.identifier.urihttp://hdl.handle.net/10754/609149en
dc.description.abstractCellulolytic microorganisms are considered to be key players in biorefinery, especially for the utilization of plant biomass. These organisms have been isolated from various environments. The Red Sea is one of the seas with high biodiversity and a unique environment, characterized by high water temperature and high salinity . However, there is little information regarding cellulases in Red Sea environments. The aim of the present study is to evaluate the Red Sea as a gene resource for microbial cellulase. I first surveyed microbial cellulases in the Red Sea using a method called metagenomes, and then investigated their abundance and diversity. My survey revealed that the Red Sea biome has a substantial abundance and a wide range of cellulase enzymes with substantial abundance, when compared with those in other environments. Next, I tried to isolate cellulase-active microorganisms from the Red Sea and I successfully obtained seven strains of four different taxonomic groups. These strains showed a similarity of 99% identity to Aspergillus ustus, 99% to Staphylococcus pasteuri, 99% to Bacillus aerius and 99% to Bacillus subtilis. The enzyme assay I conducted, revealed that these strains actually secreted active cellulases. These results suggest that the Red Sea environment can be, indeed, an excellent gene resource of microbial cellulases.en
dc.language.isoenen
dc.subjectRed Seaen
dc.subjectMetagenomeen
dc.subjectCellulasesen
dc.subjectCelluloseen
dc.subjectScreeningen
dc.titleInvestigation and Isolation of Cellulase-Producing microorganisms in the Red Seaen
dc.typeThesisen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscienceen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberArold, Stefan T.en
dc.contributor.committeememberMineta, Katsuhikoen
thesis.degree.disciplineBioscienceen
thesis.degree.nameMaster of Scienceen
dc.person.id132605en
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