Removal and Degradation Pathways of Sulfamethoxazole Present in Synthetic Municipal Wastewater via an Anaerobic Membrane Bioreactor

Handle URI:
http://hdl.handle.net/10754/609148
Title:
Removal and Degradation Pathways of Sulfamethoxazole Present in Synthetic Municipal Wastewater via an Anaerobic Membrane Bioreactor
Authors:
Sanchez Huerta, Claudia ( 0000-0002-8963-6034 )
Abstract:
The current global water crisis in addition to continues contamination of natural water bodies with harmful organic micropollutants (OMPs) have driven the development of new water treatment technologies that allow the efficient removal of such compounds. Among a long list of OMPs, antibiotics are considered as top priority pollutants to be treated due to their great resistance to biological treatments and their potential to develop bacterial resistance. Different approaches, such as membrane-based and advance oxidation processes have been proposed to alleviate or minimize antibiotics discharge into aquatic environments. However most of these processes are costly and generate either matrices with high concentration of OMPs or intermediate products with potentially greater toxicity or persistence. Therefore, this thesis proposes the study of an anaerobic membrane bioreactor (AnMBR) for the treatment of synthetic municipal wastewater containing sulfamethoxazole (SMX), a world widely used antibiotic. Besides the general evaluation of AnMBR performance in the COD removal and biogas production, this research mainly focuses on the SMX removal and its degradation pathway. Thus 5 SMX quantification was performed through solid phase extraction-liquid chromatography/mass spectrometry and the identification of its transformation products (TPs) was assessed by gas chromatography/mass spectrometry technique. The results achieved showed that, working under optimal conditions (35°C, pH 7 and ORP around -380 to -420 mV) and after a biomass adaptation period (maintaining 0.85 VSS/TSS ratio), the AnMBR process provided over 95% COD removal and 95-98% SMX removal, while allowing stable biogas composition and methane production (≈130 mL CH4/g CODremoved). Kinetic analysis through a batch test showed that after 24 h of biological reaction, AnMBR process achieved around 94% SMX removal, indicating a first order kinetic reaction with K= 0.119, which highlights the high degradation capacity of the anaerobic bacteria. Along the AnMBR process, 7 TPs were identified and possible degradation pathways were proposed. At low influent SMX concentrations (<10ppb), the only TPs detected was (1) Benzene sulfonamide N-Butyl. However, as the influent SMX concentration increased, it was possible to identify (2) Sulfanilamide, (3) Sulfisomidine and (4) 4-Aminothiophenol. Further degradation of compounds 2, 3 and 4 were detected after 9 hours of biological reaction in a batch test, producing three new intermediate products: (5) Aniline, (6) 4-Pyrimidinamine, 2,6-dimethyl and (7) Acetamide, N-(4-mercaptophenyl). Most of the detected TPs present a less complex structure than SMX, which can be associates with a lower toxicity.
Advisors:
Leiknes, TorOve ( 0000-0003-4046-5622 )
Committee Member:
Hong, Pei-Ying ( 0000-0002-4474-6600 ) ; Saikaly, Pascal ( 0000-0001-7678-3986 ) ; Wei, Chunhai
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Environmental Science and Engineering Program
Program:
Environmental Science and Engineering
Issue Date:
May-2016
Type:
Thesis
Appears in Collections:
Environmental Science and Engineering Program; Theses; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.advisorLeiknes, TorOveen
dc.contributor.authorSanchez Huerta, Claudiaen
dc.date.accessioned2016-05-12T06:42:58Zen
dc.date.available2016-05-12T06:42:58Zen
dc.date.issued2016-05en
dc.identifier.urihttp://hdl.handle.net/10754/609148en
dc.description.abstractThe current global water crisis in addition to continues contamination of natural water bodies with harmful organic micropollutants (OMPs) have driven the development of new water treatment technologies that allow the efficient removal of such compounds. Among a long list of OMPs, antibiotics are considered as top priority pollutants to be treated due to their great resistance to biological treatments and their potential to develop bacterial resistance. Different approaches, such as membrane-based and advance oxidation processes have been proposed to alleviate or minimize antibiotics discharge into aquatic environments. However most of these processes are costly and generate either matrices with high concentration of OMPs or intermediate products with potentially greater toxicity or persistence. Therefore, this thesis proposes the study of an anaerobic membrane bioreactor (AnMBR) for the treatment of synthetic municipal wastewater containing sulfamethoxazole (SMX), a world widely used antibiotic. Besides the general evaluation of AnMBR performance in the COD removal and biogas production, this research mainly focuses on the SMX removal and its degradation pathway. Thus 5 SMX quantification was performed through solid phase extraction-liquid chromatography/mass spectrometry and the identification of its transformation products (TPs) was assessed by gas chromatography/mass spectrometry technique. The results achieved showed that, working under optimal conditions (35°C, pH 7 and ORP around -380 to -420 mV) and after a biomass adaptation period (maintaining 0.85 VSS/TSS ratio), the AnMBR process provided over 95% COD removal and 95-98% SMX removal, while allowing stable biogas composition and methane production (≈130 mL CH4/g CODremoved). Kinetic analysis through a batch test showed that after 24 h of biological reaction, AnMBR process achieved around 94% SMX removal, indicating a first order kinetic reaction with K= 0.119, which highlights the high degradation capacity of the anaerobic bacteria. Along the AnMBR process, 7 TPs were identified and possible degradation pathways were proposed. At low influent SMX concentrations (<10ppb), the only TPs detected was (1) Benzene sulfonamide N-Butyl. However, as the influent SMX concentration increased, it was possible to identify (2) Sulfanilamide, (3) Sulfisomidine and (4) 4-Aminothiophenol. Further degradation of compounds 2, 3 and 4 were detected after 9 hours of biological reaction in a batch test, producing three new intermediate products: (5) Aniline, (6) 4-Pyrimidinamine, 2,6-dimethyl and (7) Acetamide, N-(4-mercaptophenyl). Most of the detected TPs present a less complex structure than SMX, which can be associates with a lower toxicity.en
dc.language.isoenen
dc.subjectAnaerobic MBRen
dc.subjectsulfamethoxazoleen
dc.subjectDegradation Pathwaysen
dc.subjectWastewater Treatmenten
dc.subjectOrganic Micropollutantsen
dc.subjectWaste wateren
dc.titleRemoval and Degradation Pathways of Sulfamethoxazole Present in Synthetic Municipal Wastewater via an Anaerobic Membrane Bioreactoren
dc.typeThesisen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentEnvironmental Science and Engineering Programen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberHong, Pei-Yingen
dc.contributor.committeememberSaikaly, Pascalen
dc.contributor.committeememberWei, Chunhaien
thesis.degree.disciplineEnvironmental Science and Engineeringen
thesis.degree.nameMaster of Scienceen
dc.person.id133286en
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